The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.
In situ detection of sequence-specific DNA binding activity specified by a recombinant bacteriophage.
Open accessAbstract
We have used a recombinant bacteriophage that expresses the DNA-binding domain of C/EBP to optimize conditions for a screening technique that may facilitate the cloning of genes that encode sequence-specific DNA-binding proteins. The method relies on the expression of cDNA inserts in bacteriophage lambda gt11. Fusion protein adsorbed onto nitrocellulose filters is probed with radioactive, double-stranded DNA as a ligand. Two procedures greatly increase the level of binding between ligand and recombinant fusion protein. First, nitrocellulose filters are processed through a denaturation/renaturation regimen using 6 M guanidine hydrochloride. Second, synthetic DNA corresponding to the specific binding site is catenated extensively using DNA ligase. The combination of these procedures leads to remarkably strong detection signals. Specific DNA-binding signals can be detected on duplicate filters, and filters can be washed and reused by repeating the cycle of denaturation/renaturation.
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Cites
Johnson, P. F., Landschulz, W. H., Graves, B. J., & McKnight, S. L. (1987) · Genes & Development · doi:10.1101/gad.1.2.133 · Cited by 595
Self-citationLin, S.-Y., & Riggs, A. D. (1975) · Cell · doi:10.1016/0092-8674(75)90116-6 · Cited by 338
Cited by
Kamei, Y., Xu, L., Heinzel, T., Torchia, J., Kurokawa, R., Gloss, B., Lin, S.-C., Heyman, R. A., Rose, D. W., Glass, C. K., et al. (1996) · Cell · doi:10.1016/s0092-8674(00)81118-6 · Cited by 2,150
Lewis, J. D., Meehan, R. R., Henzel, W. J., Maurer-Fogy, I., Jeppesen, P., Klein, F., & Bird, A. (1992) · Cell · doi:10.1016/0092-8674(92)90610-o · Cited by 1,371
Homology of the eyeless Gene of Drosophila to the Small eye Gene in Mice and Aniridia in Humans
Quiring, R., Walldorf, U., Kloter, U., & Gehring, W. J. (1994) · Science · doi:10.1126/science.7914031 · Cited by 1,100
Xiong, Y., Zhang, H., & Beach, D. (1992) · Cell · doi:10.1016/0092-8674(92)90518-h · Cited by 1,021
Schoenherr, C. J., & Anderson, D. J. (1995) · Science · doi:10.1126/science.7871435 · Cited by 976
Metadata
- Authors
- Vinson, C. R., LaMarco, K. L., Johnson, P. F., Landschulz, W. H., & McKnight, S. L.
- Venue
- Genes & Development
- Publisher
- Cold Spring Harbor Laboratory
- Published
- 1988-07
- Volume
- 2
- Issue
- 7
- Pages
- 801-806
- ISSN
- 0890-9369 (ISSN-L)
- 1549-5477
- OpenAlex
- W2072276788
- Cited by
- 506
- References
- 2
- Type
- posted content
- Language
- en
- Open access
- diamond
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- Vinson, C. R., LaMarco, K. L., Johnson, P. F., Landschulz, W. H., & McKnight, S. L. (1988). In situ detection of sequence-specific DNA binding activity specified by a recombinant bacteriophage. Genes & Development. https://doi.org/10.1101/gad.2.7.801
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