Cited by 1,371Epigenetics and DNA MethylationGenomics and Chromatin DynamicsCancer-related gene regulation

Purification, sequence, and cellular localization of a novel chromosomal protein that binds to Methylated DNA

Lewis, Joe D., Meehan, Richard R., Henzel, William J., Maurer-Fogy, Ingrid, Jeppesen, Peter, Klein, Franz, Bird, Adrian·1992·Cell

Abstract

Methylation of mammalian DNA can lead to repression of transcription and alteration of chromatin structure. Recent evidence suggests that both effects are the result of an interaction between the methylated sites and methyl-CpG-binding proteins (MeCPs). MeCP1 has previously been detected in crude nuclear extracts. Here we report the identification, purification, and cDNA cloning of a novel MeCP called MeCP2. Unlike MeCP1, the new protein is able to bind to DNA that contains a single methyl-CpG pair. By staining with an antibody, we show that the distribution of MeCP2 along the chromosomes parallels that of methyl-CpG. In mouse, for example, MeCP2 is concentrated in pericentromeric heterochromatin, which contains a large fraction (about 40%) of all genomic 5-methylcytosine.

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Crossref · 10.1016/0092-8674(92)90610-o

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  • MeCP2 is an abundant nuclear protein that is ubiquitously expressed throughout all human tissues, but is particularly abundant within neurons.

    “MeCP2 is an abundant nuclear protein that was originally identified in a screen for proteins with methyl-DNA-specific binding activity. It is ubiquitously expressed throughout all human tissues, but is particularly abundant within neurons [52,53].”

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Authors
Lewis, J. D., Meehan, R. R., Henzel, W. J., Maurer-Fogy, I., Jeppesen, P., Klein, F., & Bird, A.
Affiliations
  • Research Institute of Molecular Pathology (AT)
  • Genentech (US)
  • Western General Hospital (GB)
  • University of Vienna (AT)
Venue
Cell
Publisher
Elsevier BV
Published
1992-06
Volume
69
Issue
6
Pages
905-914
ISSN
0092-8674 (ISSN-L)
OpenAlex
W1975791071
Cited by
1,371
References
2
Citations by year
2026: 27 · 2025: 31 · 2024: 33 · 2023: 31 · 2022: 39 · 2021: 48 · 2020: 36 · 2019: 45 · 2018: 51 · 2017: 47 · 2016: 56 · 2015: 38 · 2014: 57 · 2013: 74 · 2012: 42
FWCI
10.32
Type
journal article
Language
en
Open access
closed
Locations
  • Cell (publishedVersion)
  • PubMed (publishedVersion)
Funders
Wellcome Trust
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Epigenetics and DNA Methylation
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Cancer-related gene regulation
Keywords
MeCP2, methyl-CpG binding proteins, DNA methylation, chromatin structure, transcriptional repression, pericentromeric heterochromatin, 5-methylcytosine, protein purification, cDNA cloning, protein localization
MeSH terms
Amino Acid Sequence, Animals, Base Sequence, Chromosomal Proteins, Non-Histone, Cloning, Molecular, DNA, DNA-Binding Proteins, DNA, Satellite, Fluorescent Antibody Technique, Methylation, Molecular Sequence Data, Molecular Weight, Rats

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