Regulated polyadenylation controls mRNA translation during meiotic maturation of mouse oocytes.
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The translational activation of dormant tissue-type plasminogen activator mRNA during meiotic maturation of mouse oocytes is accompanied by elongation of its 3'-poly(A) tract. Injected RNA fragments that correspond to part of the 3'-untranslated region (3'UTR) of this mRNA are also subject to regulated polyadenylation. Chimeric mRNAs containing part of this 3'UTR are polyadenylated and translated following resumption of meiosis. Polyadenylation and translation of chimeric mRNAs require both specific sequences in the 3'UTR and the canonical 3'-processing signal AAUAAA. Injection of 3'-blocked mRNAs and in vitro polyadenylated mRNAs shows that the presence of a long poly(A) tract is necessary and sufficient for translation. These results establish a role for regulated polyadenylation in the post-transcriptional control of gene expression.
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In early embryos, longer poly(A)-tail lengths cause more efficient translation.
“We favor this last possibility because it agrees with the known importance of cytoplasmic polyadenylation for activating genes in maturing oocytes8,21,22 and early embryos23,24 of Xenopus and certain other vertebrate contexts25–30.”
From Poly(A)-tail profiling reveals an embryonic switch in translational control (2014) · cites this paper as [25]
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Poly(A)-tail profiling reveals an embryonic switch in translational control
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- Authors
- Vassalli, J. D., Huarte, J., Belin, D., Gubler, P., Vassalli, A., O'Connell, M. L., Parton, L. A., Rickles, R. J., & Strickland, S.
- Affiliations
- University of Geneva (CH)
- Venue
- Genes & Development
- Publisher
- Cold Spring Harbor Laboratory
- Published
- 1989-12-01
- Volume
- 3
- Issue
- 12b
- Pages
- 2163-2171
- ISSN
- 0890-9369 (ISSN-L)
- OpenAlex
- W2030629284
- Cited by
- 240
- References
- 48
- Citations by year
- 2025: 1 · 2024: 3 · 2023: 3 · 2022: 3 · 2021: 2 · 2020: 3 · 2019: 2 · 2018: 3 · 2017: 4 · 2016: 1 · 2015: 5 · 2014: 5 · 2013: 3 · 2012: 5
- Type
- posted content
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Genes & Development (publishedVersion)
- PubMed (publishedVersion)
- Archive ouverte UNIGE (University of Geneva) (submittedVersion)
- Archive ouverte UNIGE (University of Geneva) (submittedVersion, cc-by)
- Funders
- American Cancer Society
- Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung (25922)
- National Institutes of Health
- Eunice Kennedy Shriver National Institute of Child Health and Human Development (HD-17875, HD-25922)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Research and Splicing
- Health Sciences › Medicine › Public Health, Environmental and Occupational Health › Reproductive Biology and Fertility
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA and protein synthesis mechanisms
- Keywords
- mRNA translation, meiotic maturation, mouse oocytes, tissue plasminogen activator, 3' untranslated region, post-transcriptional regulation, chimeric mRNA
- MeSH terms
- Animals, Base Sequence, Chimera, Meiosis, Molecular Sequence Data, Oocytes, Tissue Plasminogen Activator, Poly A, RNA, RNA, Messenger, Protein Biosynthesis, Blotting, Northern, Mice
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