Cited by 240RNA Research and SplicingReproductive Biology and FertilityRNA and protein synthesis mechanisms

Regulated polyadenylation controls mRNA translation during meiotic maturation of mouse oocytes.

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Vassalli, J D, Huarte, J, Belin, D, Gubler, P, Vassalli, A, O'Connell, M L, Parton, L A, Rickles, R J +1 more·1989·Genes & Development

Abstract

The translational activation of dormant tissue-type plasminogen activator mRNA during meiotic maturation of mouse oocytes is accompanied by elongation of its 3'-poly(A) tract. Injected RNA fragments that correspond to part of the 3'-untranslated region (3'UTR) of this mRNA are also subject to regulated polyadenylation. Chimeric mRNAs containing part of this 3'UTR are polyadenylated and translated following resumption of meiosis. Polyadenylation and translation of chimeric mRNAs require both specific sequences in the 3'UTR and the canonical 3'-processing signal AAUAAA. Injection of 3'-blocked mRNAs and in vitro polyadenylated mRNAs shows that the presence of a long poly(A) tract is necessary and sufficient for translation. These results establish a role for regulated polyadenylation in the post-transcriptional control of gene expression.

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  • In early embryos, longer poly(A)-tail lengths cause more efficient translation.

    “We favor this last possibility because it agrees with the known importance of cytoplasmic polyadenylation for activating genes in maturing oocytes8,21,22 and early embryos23,24 of Xenopus and certain other vertebrate contexts25–30.”

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Authors
Vassalli, J. D., Huarte, J., Belin, D., Gubler, P., Vassalli, A., O'Connell, M. L., Parton, L. A., Rickles, R. J., & Strickland, S.
Affiliations
University of Geneva (CH)
Venue
Genes & Development
Published
1989-12-01
Volume
3
Issue
12b
Pages
2163-2171
ISSN
0890-9369 (ISSN-L)
OpenAlex
W2030629284
Cited by
240
References
48
Citations by year
2025: 1 · 2024: 3 · 2023: 3 · 2022: 3 · 2021: 2 · 2020: 3 · 2019: 2 · 2018: 3 · 2017: 4 · 2016: 1 · 2015: 5 · 2014: 5 · 2013: 3 · 2012: 5
Type
posted content
Language
en
Open access
Locations
  • Genes & Development (publishedVersion)
  • PubMed (publishedVersion)
  • Archive ouverte UNIGE (University of Geneva) (submittedVersion)
  • Archive ouverte UNIGE (University of Geneva) (submittedVersion, cc-by)
Funders
  • American Cancer Society
  • Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung (25922)
  • National Institutes of Health
  • Eunice Kennedy Shriver National Institute of Child Health and Human Development (HD-17875, HD-25922)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Research and Splicing
  • Health Sciences › Medicine › Public Health, Environmental and Occupational Health › Reproductive Biology and Fertility
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA and protein synthesis mechanisms
Keywords
mRNA translation, meiotic maturation, mouse oocytes, tissue plasminogen activator, 3' untranslated region, post-transcriptional regulation, chimeric mRNA
MeSH terms
Animals, Base Sequence, Chimera, Meiosis, Molecular Sequence Data, Oocytes, Tissue Plasminogen Activator, Poly A, RNA, RNA, Messenger, Protein Biosynthesis, Blotting, Northern, Mice

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