The DOI matches its Crossref record; 28 of the 28 references it cites were found in a registry or an index.
Mammalian sperm translate nuclear-encoded proteins by mitochondrial-type ribosomes
Open accessAbstract
It is widely accepted that spermatozoa are translationally silent. The present study demonstrates, for the first time, incorporation of labeled amino acids into polypeptides during sperm capacitation, which was completely inhibited by mitochondrial translation inhibitors but not by the cytoplasmic translation inhibitor. Unlike 80S cytoplasmic ribosomes, 55S mitochondrial ribosomes were present in polysomal fractions, indicating that these ribosomes are actively involved in protein translation in spermatozoa. Inhibition of protein translation significantly reduced sperm motility, capacitation and in vitro fertilization rate. Thus, contrary to the accepted dogma, nuclear genes are expressed as proteins in sperm during their residence in the female reproductive tract until fertilization.
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Citations
Cites
A sperm ion channel required for sperm motility and male fertility
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Spermatozoal RNA profiles of normal fertile men
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Penetration, Adhesion, and Fusion in Mammalian Sperm-Egg Interaction
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Genome‐wide analysis of mRNAs targeted to yeast mitochondria
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Self-citationMammalian oocyte growth and development in vitro
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Cited by
Ramalho-Santos, J., Varum, S., Amaral, S., Mota, P. C., Sousa, A. P., & Amaral, A. (2009) · Human Reproduction Update · doi:10.1093/humupd/dmp016 · Cited by 482
Mitochondrial DNA and the Mammalian Oocyte
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Protein synthesis in sperm: Dialog between mitochondria and cytoplasm
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Self-citationNew insights into the molecular mechanisms of sperm-egg interaction
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Metadata
- Authors
- Gur, Y., & Breitbart, H.
- Venue
- Genes & Development
- Publisher
- Cold Spring Harbor Laboratory
- Published
- 2006-01-31
- Volume
- 20
- Issue
- 4
- Pages
- 411-416
- ISSN
- 0890-9369 (ISSN-L)
- 1549-5477
- OpenAlex
- W2114037340
- Cited by
- 384
- References
- 28
- Type
- posted content
- Language
- en
- Open access
- diamond
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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