The DOI matches its Crossref record; 38 of the 38 references it cites were found in a registry or an index.
A natural antisense transcript regulates Zeb2/Sip1 gene expression during Snail1-induced epithelial–mesenchymal transition
Open accessAbstract
Expression of Snail1 in epithelial cells triggers an epithelial–mesenchymal transition (EMT). Here, we demonstrate that the synthesis of Zeb2, a transcriptional repressor of E-cadherin, is up-regulated after Snail1-induced EMT. Snail1 does not affect the synthesis of Zeb2 mRNA, but prevents the processing of a large intron located in its 5′-untranslated region (UTR). This intron contains an internal ribosome entry site (IRES) necessary for the expression of Zeb2. Maintenance of 5′-UTR Zeb2 intron is dependent on the expression of a natural antisense transcript (NAT) that overlaps the 5′ splice site in the intron. Ectopic overexpression of this NAT in epithelial cells prevents splicing of the Zeb2 5′-UTR, increases the levels of Zeb2 protein, and consequently down-regulates E-cadherin mRNA and protein. The relevance of these results is demonstrated by the strong association between NAT presence and conservation of the 5′-UTR intron in cells that have undergone EMT or in human tumors with low E-cadherin expression. Therefore, the results presented in this article reveal the existence of a NAT capable of activating Zeb2 expression, explain the mechanism involved in this activation, and demonstrate that this NAT regulates E-cadherin expression.
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The Two-Handed E Box Binding Zinc Finger Protein SIP1 Downregulates E-Cadherin and Induces Invasion
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Long non-coding RNAs: insights into functions
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A Long Noncoding RNA Controls Muscle Differentiation by Functioning as a Competing Endogenous RNA
Cesana, M., Cacchiarelli, D., Legnini, I., Santini, T., Sthandier, O., Chinappi, M., Tramontano, A., & Bozzoni, I. (2011) · Cell · doi:10.1016/j.cell.2011.09.028 · Cited by 2,554
Metadata
- Authors
- Beltran, M., Puig, I., Peña, C., García, J. M., Álvarez, A. B., Peña, R., Bonilla, F., & de Herreros, A. G.
- Venue
- Genes & Development
- Publisher
- Cold Spring Harbor Laboratory
- Published
- 2008-03-15
- Volume
- 22
- Issue
- 6
- Pages
- 756-769
- ISSN
- 0890-9369 (ISSN-L)
- 1549-5477
- OpenAlex
- W2121189598
- Cited by
- 677
- References
- 38
- Type
- posted content
- Language
- en
- Open access
- diamond
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- CoChat. (2026). Citation check: A natural antisense transcript regulates Zeb2/Sip1 gene expression during Snail1-induced epithelial–mesenchymal transition. https://app.cochat.ai/papers/10.1101/gad.455708
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- Beltran, M., Puig, I., Peña, C., García, J. M., Álvarez, A. B., Peña, R., Bonilla, F., & de Herreros, A. G. (2008). A natural antisense transcript regulates Zeb2/Sip1 gene expression during Snail1-induced epithelial–mesenchymal transition. Genes & Development. https://doi.org/10.1101/gad.455708
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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