A stem/loop in U6 RNA defines a conformational switch required for pre-mRNA splicing.
Open accessAbstract
U6 small nuclear RNA (snRNA) is an essential component of the spliceosome, the ribonucleoprotein complex that carries out the splicing of pre-mRNAs. The precise function of U6 RNA is unknown, but it has been proposed to participate directly in catalysis of the splicing reaction. We present biochemical and genetic evidence for an intramolecular stem/loop structure in the 3' half of U6 RNA of the yeast Saccharomyces cerevisiae that is mutually exclusive with the intermolecular base-pairing between U6 RNA and U4 snRNA. Strains with mutations that stabilize the U6 RNA 3'-intramolecular stem exhibit cold-sensitive growth and accumulate free U4 RNA, indicative of a block in U4/U6 snRNP assembly. The cold sensitivity can be partially suppressed by overexpression of U4 RNA. Mutations that disrupt base-pairing in the intramolecular 3' stem and mutations elsewhere in U6 RNA also suppress the growth defect. We conclude that a large conformational switch, involving melting of the U6 RNA 3' stem, is required for U4/U6 snRNP assembly. We hypothesize that formation of the U6 RNA intramolecular 3' stem after U4 RNA leaves the assembled spliceosome serves to activate U6 RNA for splicing by juxtaposing regions in U6 RNA that interact with U2 small nuclear RNA.
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
rs2807267 lies within an enhancer peak specific for T cells and is 948bps downstream of the RNA U6 small nuclear 320 pseudogene (RNU6-320P).
“This variant lies within an enhancer peak specific for T cells and is 948bps downstream of the RNA U6 small nuclear 320 pseudogene (RNU6-320P), a component of the U6 small nuclear ribonucleoprotein (snRNP) that is part of the spliceosome and is responsible for the splicing of introns from pre-mRNA (14) (Fig. 2B).”
From Multiple sclerosis genomic map implicates peripheral immune cells and microglia in susceptibility (2019) · cites this paper as [14]
The Abstract (page not provided) says U6 small nuclear RNA is an essential component of the spliceosome, which splices pre-mRNA. It does not identify RNU6-320P or establish that this pseudogene is a functional spliceosomal component. The variant’s enhancer and distance annotations are also not addressed in this paper. (LLM, conf=0.88)
Experimental — accuracy not yet measured
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Cites
Sikorski, R. S., & Hieter, P. (1989) · Genetics · doi:10.1093/genetics/122.1.19 · Cited by 8,685 · see citing works
Transformation of intact yeast cells treated with alkali cations
Ito, H., Fukuda, Y., Murata, K., & Kimura, A. (1983) · Journal of Bacteriology · doi:10.1128/jb.153.1.163-168.1983 · Cited by 7,518 · see citing works
[19] Rapid and efficient site-specific mutagenesis without phenotypic selection
Kunkel, T. A., Roberts, J. D., & Zakour, R. A. (1987) · Methods in enzymology on CD-ROM/Methods in enzymology · doi:10.1016/0076-6879(87)54085-x · Cited by 5,341 · see citing works
[1] Production of single-stranded plasmid DNA
Vieira, J., & Messing, J. W. (1987) · Methods in enzymology on CD-ROM/Methods in enzymology · doi:10.1016/0076-6879(87)53044-0 · Cited by 2,558 · see citing works
Hoffman, C. S., & Winston, F. (1987) · Gene · doi:10.1016/0378-1119(87)90131-4 · Cited by 2,492 · see citing works
Multifunctional yeast high-copy-number shuttle vectors
Christianson, T., Sikorski, R. S., Dante, M., Shero, J. H., & Hieter, P. (1992) · Gene · doi:10.1016/0378-1119(92)90454-w · Cited by 1,660 · see citing works
Improved free-energy parameters for predictions of RNA duplex stability.
Freier, S. M., Kierzek, R., Jaeger, J. A., Sugimoto, N., Caruthers, M. H., Neilson, T., & Turner, D. H. (1986) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.83.24.9373 · Cited by 1,470 · see citing works
Biochemical Mechanisms of Constitutive and Regulated Pre-mRNA Splicing
Green, M. R. (1991) · Annual Review of Cell Biology · doi:10.1146/annurev.cb.07.110191.003015 · Cited by 711 · see citing works
[20] In vitro mutagenesis and plasmid shuffling: From cloned gene to mutant yeast
Sikorski, R. S., & Boeke, J. D. (1991) · Methods in enzymology on CD-ROM/Methods in enzymology · doi:10.1016/0076-6879(91)94023-6 · Cited by 583 · see citing works
Moazed, D., Stern, S., & Noller, H. F. (1986) · Journal of Molecular Biology · doi:10.1016/0022-2836(86)90441-9 · Cited by 544 · see citing works
Interactions between small nuclear ribonucleoprotein particles in formation of spliceosomes
Konarska, M. M., & Sharp, P. A. (1987) · Cell · doi:10.1016/0092-8674(87)90614-3 · Cited by 516 · see citing works
Parker, R., Siliciano, P. G., & Guthrie, C. (1987) · Cell · doi:10.1016/0092-8674(87)90564-2 · Cited by 513 · see citing works
Guthrie, C., & Patterson, B. (1988) · Annual Review of Genetics · doi:10.1146/annurev.ge.22.120188.002131 · Cited by 476 · see citing works
A self-splicing RNA excises an intron lariat
Peebles, C. L., Perlman, P. S., Mecklenburg, K. L., Petrillo, M. L., Tabor, J., Jarrell, K. A., & Cheng, H.-L. (1986) · Cell · doi:10.1016/0092-8674(86)90755-5 · Cited by 447 · see citing works
Madhani, H. D., & Guthrie, C. (1992) · Cell · doi:10.1016/0092-8674(92)90556-r · Cited by 438 · see citing works
Spliceosomal RNA U6 is remarkably conserved from yeast to mammals
Brow, D. A., & Guthrie, C. (1988) · Nature · doi:10.1038/334213a0 · Cited by 436 · see citing works
Self-citationMessenger RNA Splicing in Yeast: Clues to Why the Spliceosome Is a Ribonucleoprotein
Guthrie, C. (1991) · Science · doi:10.1126/science.1853200 · Cited by 430 · see citing works
Interactions of Small Nuclear RNA's with Precursor Messenger RNA During in Vitro Splicing
Wassarman, D. A., & STEITZ, J. A. (1992) · Science · doi:10.1126/science.1411506 · Cited by 372 · see citing works
Spliceosome assembly in yeast.Preprint
Cheng, S.-C., & Abelson, J. N. (1987) · Genes & Development · doi:10.1101/gad.1.9.1014 · Cited by 366 · see citing works
van der Veen, R., Arnberg, A. C., van der Horst, G. T. J., Bonen, L., Tabak, H. F., & Grivell, L. A. (1986) · Cell · doi:10.1016/0092-8674(86)90756-7 · Cited by 343 · see citing works
Inoue, T., & Cech, T. R. (1985) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.82.3.648 · Cited by 321 · see citing works
Structure of an adenine˙cytosine base pair in DNA and its implications for mismatch repair
Hunter, W. N., Brown, T., Anand, N. N., & Kennard, O. (1986) · Nature · doi:10.1038/320552a0 · Cited by 281 · see citing works
Pikielny, C. W., Rymond, B. C., & Rosbash, M. (1986) · Nature · doi:10.1038/324341a0 · Cited by 280 · see citing works
RNA recognition: A family matter?
Mattaj, I. W. (1993) · Cell · doi:10.1016/0092-8674(93)90265-r · Cited by 252 · see citing works
Elledge, S. J., & Davis, R. W. (1988) · Gene · doi:10.1016/0378-1119(88)90202-8 · Cited by 222 · see citing works
SELECTION OF lys2 MUTANTS OF THE YEAST SACCHAROMYCES CEREVISIAE BY THE UTILIZATION OF α-AMINOADIPATE
Chattoo, B. B., Sherman, F., Azubalis, D. A., Fjellstedt, T. A., Mehnert, D., & Ogur, M. (1979) · Genetics · doi:10.1093/genetics/93.1.51 · Cited by 222 · see citing works
U4 and U6 RNAs coexist in a single small nuclear ribonucleoprotein particle
Hashimoto, C., & STEITZ, J. A. (1984) · Nucleic Acids Research · doi:10.1093/nar/12.7.3283 · Cited by 219 · see citing works
Bringmann, P., Appel, B., Rinke, J., Reuter, R., Theissen, H., & Lührmann, R. (1984) · The EMBO Journal · doi:10.1002/j.1460-2075.1984.tb01977.x · Cited by 200 · see citing works
Zillmann, M., Zapp, M. L., & Berget, S. M. (1988) · Molecular and Cellular Biology · doi:10.1128/mcb.8.2.814 · Cited by 191 · see citing works
Multiple roles for U6 snRNA in the splicing pathway.Preprint
Madhani, H. D., Bordonné, R., & Guthrie, C. (1990) · Genes & Development · doi:10.1101/gad.4.12b.2264 · Cited by 179 · see citing works
Spliceosome assembly involves the binding and release of U4 small nuclear ribonucleoprotein.
Lamond, A. I., Konarska, M. M., Grabowski, P. J., & Sharp, P. A. (1988) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.85.2.411 · Cited by 178 · see citing works
Base pairing between U2 and U6 snRNAs is necessary for splicing of a mammalian pre-mRNA
Wu, J., & Manley, J. L. (1991) · Nature · doi:10.1038/352818a0 · Cited by 175 · see citing works
Fabrizio, P., & Abelson, J. N. (1990) · Science · doi:10.1126/science.2145630 · Cited by 170 · see citing works
Sawa, H., & Abelson, J. N. (1992) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.89.23.11269 · Cited by 168 · see citing works
Genetic evidence for base pairing between U2 and U6 snRNA in mammalian mRNA splicing
Datta, B., & Weiner, A. M. (1991) · Nature · doi:10.1038/352821a0 · Cited by 165 · see citing works
Evidence for base-pairing between mammalian U2 and U6 small nuclear ribonucleoprotein particles.Preprint
Hausner, T. P., Giglio, L. M., & Weiner, A. M. (1990) · Genes & Development · doi:10.1101/gad.4.12a.2146 · Cited by 155 · see citing works
Brow, D. A., & Guthrie, C. (1990) · Genes & Development · doi:10.1101/gad.4.8.1345 · Cited by 152 · see citing works
Self-citationAres, M., & Igel, A. H. (1990) · Genes & Development · doi:10.1101/gad.4.12a.2132 · Cited by 149 · see citing works
The nucleotide sequence of nuclear U6 (4.7 S) RNA.
Epstein, P. N., Reddy, R., Henning, D., & Busch, H. (1980) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)43587-9 · Cited by 145 · see citing works
Suppressors of a U4 snRNA mutation define a novel U6 snRNP protein with RNA-binding motifs.Preprint
Shannon, K. W., & Guthrie, C. (1991) · Genes & Development · doi:10.1101/gad.5.5.773 · Cited by 145 · see citing works
Association of U6 snRNA with the 5'-splice site region of pre-mRNA in the spliceosome.Preprint
Sawa, H., & Shimura, Y. (1992) · Genes & Development · doi:10.1101/gad.6.2.244 · Cited by 142 · see citing works
Rinke, J., Appel, B., Digweed, M., & Lührmann, R. (1985) · Journal of Molecular Biology · doi:10.1016/0022-2836(85)90057-9 · Cited by 131 · see citing works
Vankan, P., McGuigan, C., & Mattaj, I. W. (1990) · The EMBO Journal · doi:10.1002/j.1460-2075.1990.tb07541.x · Cited by 129 · see citing works
Yeast contains small nuclear RNAs encoded by single copy genes
Wise, J. A., Tollervey, D. W., Maloney, D. H., Swerdlow, H., Dunn, E. J., & Guthrie, C. (1983) · Cell · doi:10.1016/0092-8674(83)90107-1 · Cited by 125 · see citing works
Mottram, J. C., Perry, K. L., Lizardi, P. M., Lührmann, R., Agabian, N. M., & Nelson, R. G. (1989) · Molecular and Cellular Biology · doi:10.1128/mcb.9.3.1212 · Cited by 117 · see citing works
Solution conformation of an RNA hairpin loop
Puglisi, J. D., Wyatt, J. R., & Tinoco, I. (1990) · Biochemistry · doi:10.1021/bi00469a026 · Cited by 110 · see citing works
Compilation of small RNA sequences
Gu, J. H., & Reddy, R. (1994) · Nucleic Acids Research · doi:10.1093/nar/22.17.3481 · Cited by 107 · see citing works
A cold-sensitive mutation in 16S rRNA provides evidence for helical switching in ribosome assembly.Preprint
Dammel, C., & Noller, H. F. (1993) · Genes & Development · doi:10.1101/gad.7.4.660 · Cited by 102 · see citing works
Siliciano, P. G., Brow, D. A., Roiha, H., & Guthrie, C. (1987) · Cell · doi:10.1016/0092-8674(87)90031-6 · Cited by 102 · see citing works
Self-citationYean, S.-L., & Lin, R.-J. (1991) · Molecular and Cellular Biology · doi:10.1128/mcb.11.11.5571 · Cited by 98 · see citing works
Architecture of a Yeast U6 RNA Gene Promoter
Eschenlauer, J. B., Kaiser, M. W., Gerlach, V. L., & Brow, D. A. (1993) · Molecular and Cellular Biology · doi:10.1128/mcb.13.5.3015 · Cited by 94 · see citing works
Self-citationBrow, D. A., & Guthrie, C. (1989) · Nature · doi:10.1038/337014a0 · Cited by 90 · see citing works
Self-citationThe nucleotide sequence of nuclear 4.8S RNA of mouse cells
Harada, F., Kato, N., & Nishimura, S. (1980) · Biochemical and Biophysical Research Communications · doi:10.1016/0006-291x(80)91620-4 · Cited by 88 · see citing works
Wang, C., Gao, H., Gaffney, B. L., & Jones, R. A. (1991) · Journal of the American Chemical Society · doi:10.1021/ja00014a068 · Cited by 72 · see citing works
Efficient association of U2 snRNPs with pre-mRNA requires an essential U2 RNA structural element.Preprint
Zavanelli, M. I., & Ares, M. (1991) · Genes & Development · doi:10.1101/gad.5.12b.2521 · Cited by 66 · see citing works
Wolff, T., & Bindereif, A. (1993) · Genes & Development · doi:10.1101/gad.7.7b.1377 · Cited by 58 · see citing works
Zavanelli, M. I., Britton, J. S., Igel, A. H., & Ares, M. (1994) · Molecular and Cellular Biology · doi:10.1128/mcb.14.3.1689 · Cited by 47 · see citing works
A rapid assay for quantitative detection of specific RNAs
Li, Z., & Brow, D. A. (1993) · Nucleic Acids Research · doi:10.1093/nar/21.19.4645 · Cited by 46 · see citing works
Self-citationSmall nuclear rnas from budding yeasts: phylogenetic comparisons reveal extensive size variation
Roiha, H., Shuster, E. O., Brow, D. A., & Guthrie, C. (1989) · Gene · doi:10.1016/0378-1119(89)90038-3 · Cited by 31 · see citing works
Self-citationThe U6 small nuclear RNA fromTrypanosoma brucei
Tschudi, C., Krainer, A. R., & Ullu, E. (1988) · Nucleic Acids Research · doi:10.1093/nar/16.23.11375 · Cited by 29 · see citing works
Miura, K., Tsuda, S., Harada, F., & Ueda, T. (1983) · Nucleic Acids Research · doi:10.1093/nar/11.17.5893 · Cited by 8 · see citing works
Compilation of small RNA sequences
Shumyatsky, G. P., & Reddy, R. (1993) · Nucleic Acids Research · doi:10.1093/nar/21.13.3017 · Cited by 4 · see citing works
Flagged first, then by citations
Research works printed but not linked: 3
- Rymond, B.C. and M. Rosbash. 1992. Yeast pre-mRNA splicing. The molecular and cellular biology of the yeast Saccharo- myces: Gene expression, (eds. E.W. Jones, J.R. Pringle, and J.R. Broach) pp. 143-192. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York.
- Treco, D.A. 1989. Saccharomyces cerevisiae, Current protocols in molecular biology (eds F.M. Ausubel, R. Brent, R.E. Kings- ton, D.D. Moore, J.G. Seidman, J.A. Smith, and K. Struhl), pp. 13.12.1-13.12.3. Greene Publishing Associates and Wiley-Interscience, New York.
- Zavanelli, M.I., J.S. Britton, A.H. Igel, and M. Ares, Jr. 1994. Mutations in an essential U2 snRNA structure cause cold sensitive U2 snRNP function by favoring competing alter- native U2 RNA structures. Mol. Cell. Biol, (in press).
Cited by
Sorted by citations
Multiple sclerosis genomic map implicates peripheral immune cells and microglia in susceptibility
International Multiple Sclerosis Genetics Consortium, Patsopoulos, N. A., Baranzini, S. E., Santaniello, A., Shoostari, P., Cotsapas, C., Wong, G., Beecham, A. H., James, T., Replogle, J., et al. (2019) · Science · doi:10.1126/science.aav7188 · Cited by 1,471 · see citing works
Splicing double: insights from the second spliceosome
Patel, A. A., & STEITZ, J. A. (2003) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm1259 · Cited by 479 · see citing works
Allosteric Cascade of Spliceosome Activation
Brow, D. A. (2002) · Annual Review of Genetics · doi:10.1146/annurev.genet.36.043002.091635 · Cited by 372 · see citing works
Self-citationHighly Diverged U4 and U6 Small Nuclear RNAs Required for Splicing Rare AT-AC Introns
Tarn, W., & STEITZ, J. A. (1996) · Science · doi:10.1126/science.273.5283.1824 · Cited by 227 · see citing works
Pre-mRNA splicing: the discovery of a new spliceosome doubles the challenge
Tarn, W., & STEITZ, J. A. (1997) · Trends in Biochemical Sciences · doi:10.1016/s0968-0004(97)01018-9 · Cited by 221 · see citing works
Ribozyme-catalyzed tRNA aminoacylation
Lee (2000) · Nature Structural Biology · doi:10.1038/71225 · Cited by 191 · see citing works
A novel U2-U6 snRNA structure is necessary for mammalian mRNA splicing.Preprint
Sun, J., & Manley, J. L. (1995) · Genes & Development · doi:10.1101/gad.9.7.843 · Cited by 193 · see citing works
Genetics of Multiple Sclerosis: An Overview and New DirectionsPreprint
Patsopoulos, N. A. (2018) · Cold Spring Harbor Perspectives in Medicine · doi:10.1101/cshperspect.a028951 · Cited by 177 · see citing works
Metal binding and base ionization in the U6 RNA intramolecular stem-loop structure
Huppler, A. R., Nikstad, L. J., Allmann, A. M., Brow, D. A., & Butcher, S. E. (2002) · Nature Structural Biology · doi:10.1038/nsb800 · Cited by 152 · see citing works
Self-citationU2–U6 RNA folding reveals a group II intron-like domain and a four-helix junction
Sashital, D. G., Cornilescu, G., & Butcher, S. E. (2004) · Nature Structural & Molecular Biology · doi:10.1038/nsmb863 · Cited by 139 · see citing works
The life of U6 small nuclear RNA, from cradle to grave
Didychuk, A. L., Butcher, S. E., & Brow, D. A. (2018) · RNA · doi:10.1261/rna.065136.117 · Cited by 123 · see citing works
Self-citationU2 toggles iteratively between the stem IIa and stem IIc conformations to promote pre-mRNA splicingPreprint
Hilliker, A. K., Mefford, M. A., & Staley, J. P. (2007) · Genes & Development · doi:10.1101/gad.1536107 · Cited by 109 · see citing works
Posttranscriptional Modifications in the U Small Nuclear RNAs
Massenet, S., Mougin, A., & Branlant, C. (2014) · ASM Press eBooks · doi:10.1128/9781555818296.ch11 · Cited by 99 · see citing works
Structure of the yeast U2/U6 snRNA complex
Burke, J. E., Sashital, D. G., Zuo, X., Wang, Y., & Butcher, S. E. (2012) · RNA · doi:10.1261/rna.031138.111 · Cited by 90 · see citing works
Invariant U2 snRNA Nucleotides Form a Stem Loop to Recognize the Intron Early in Splicing
Perriman, R., & Ares, M. (2010) · Molecular Cell · doi:10.1016/j.molcel.2010.02.036 · Cited by 86 · see citing works
Distinct domains of splicing factor Prp8 mediate different aspects of spliceosome activation
Kuhn, A. N., Reichl, E. M., & Brow, D. A. (2002) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.102304299 · Cited by 82 · see citing works
Self-citationA conserved Lsm-interaction motif in Prp24 required for efficient U4/U6 di-snRNP formation
Rader, S. D., & Guthrie, C. (2002) · RNA · doi:10.1017/s1355838202020010 · Cited by 82 · see citing works
Rearrangement of snRNA Structure during Assembly and Function of the Spliceosome
Ares, M., & Weiser, B. (1995) · Progress in nucleic acid research and molecular biology · doi:10.1016/s0079-6603(08)60813-2 · Cited by 76 · see citing works
Core structure of the U6 small nuclear ribonucleoprotein at 1.7-Å resolution
Montemayor, E., Curran, E. C., Liao, H. H., Andrews, K. L., Treba, C. N., Butcher, S. E., & Brow, D. A. (2014) · Nature Structural & Molecular Biology · doi:10.1038/nsmb.2832 · Cited by 72 · see citing works
Self-citationRasche, N., Dybkov, O., Schmitzová, J., Akyildiz, B., Fabrizio, P., & Lührmann, R. (2012) · The EMBO Journal · doi:10.1038/emboj.2011.502 · Cited by 71 · see citing works
Dynamics in the U6 RNA Intramolecular Stem−Loop: A Base Flipping Conformational Change,
Reiter, N. J., Blad, H., Abildgaard, F., & Butcher, S. E. (2004) · Biochemistry · doi:10.1021/bi048815y · Cited by 70 · see citing works
Alternative yeasts for winemaking:Saccharomycesnon-cerevisiaeand its hybrids
Pérez‐Torrado, R., Barrio, E., & Querol, A. (2017) · Critical Reviews in Food Science and Nutrition · doi:10.1080/10408398.2017.1285751 · Cited by 69 · see citing works
Gerlach, V. L., Whitehall, S. K., Geiduschek, E. P., & Brow, D. A. (1995) · Molecular and Cellular Biology · doi:10.1128/mcb.15.3.1455 · Cited by 68 · see citing works
Self-citationDong, Y., & Ares, M. (1996) · Molecular and Cellular Biology · doi:10.1128/mcb.16.3.818 · Cited by 66 · see citing works
Probing protein surface accessibility with solvent and paramagnetic molecules
Bernini, A., Venditti, V., Spiga, O., & Niccolai, N. (2008) · Progress in Nuclear Magnetic Resonance Spectroscopy · doi:10.1016/j.pnmrs.2008.10.003 · Cited by 64 · see citing works
Jandrositz, A., & Guthrie, C. (1995) · The EMBO Journal · doi:10.1002/j.1460-2075.1995.tb07060.x · Cited by 63 · see citing works
Anokhina, M. M., Bessonov, S., Miao, Z., Westhof, É., Hartmuth, K., & Lührmann, R. (2013) · The EMBO Journal · doi:10.1038/emboj.2013.198 · Cited by 59 · see citing works
Marsolier‐Kergoat, M., Tanaka, S., Livingstone-Zatchej, M., Grunstein, M. R., Thoma, F., & Sentenac, A. (1995) · Genes & Development · doi:10.1101/gad.9.4.410 · Cited by 59 · see citing works
RNA Structure and RNA–Protein Interactions in Purified Yeast U6 snRNPs
Karaduman, R., Fabrizio, P., Hartmuth, K., Urlaub, H., & Lührmann, R. (2005) · Journal of Molecular Biology · doi:10.1016/j.jmb.2005.12.013 · Cited by 58 · see citing works
Weidenhammer, E. M., Ruiz-Noriega, M., & Woolford, J. L. (1997) · Molecular and Cellular Biology · doi:10.1128/mcb.17.7.3580 · Cited by 58 · see citing works
Structural basis for heterogeneous kinetics: Reengineering the hairpin ribozyme
Esteban, J. A., Walter, N. G., Kotzorek, G., Heckman, J. E., & Burke, J. M. (1998) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.95.11.6091 · Cited by 55 · see citing works
Biochemical characterization of a U6 small nuclear RNA‐specific terminal uridylyltransferase
Trippe, R., Richly, H., & Benecke, B. (2003) · European Journal of Biochemistry · doi:10.1046/j.1432-1033.2003.03466.x · Cited by 52 · see citing works
Zavanelli, M. I., Britton, J. S., Igel, A. H., & Ares, M. (1994) · Molecular and Cellular Biology · doi:10.1128/mcb.14.3.1689 · Cited by 47 · see citing works
A novel occluded RNA recognition motif in Prp24 unwinds the U6 RNA internal stem loop
Martin-Tumasz, S., Richie, A. C., Clos, L. J., Brow, D. A., & Butcher, S. E. (2011) · Nucleic Acids Research · doi:10.1093/nar/gkr455 · Cited by 44 · see citing works
Self-citationStructure and Interactions of the First Three RNA Recognition Motifs of Splicing Factor Prp24
Bae, E., Reiter, N. J., Bingman, C. A., Kwan, S. S., Lee, D., Phillips, G. N., Butcher, S. E., & Brow, D. A. (2007) · Journal of Molecular Biology · doi:10.1016/j.jmb.2007.01.078 · Cited by 44 · see citing works
Self-citationMougin, A., GOTTSCHALK, A. R., Fabrizio, P., Lührmann, R., & Branlant, C. (2002) · Journal of Molecular Biology · doi:10.1006/jmbi.2002.5451 · Cited by 44 · see citing works
Free Energy Landscapes of RNA/RNA Complexes: With Applications to snRNA Complexes in Spliceosomes
Cao, S., & Chen, S. (2005) · Journal of Molecular Biology · doi:10.1016/j.jmb.2005.12.014 · Cited by 43 · see citing works
Tronchoni, J., Medina, V., Guillamón, J. M., Querol, A., & Pérez‐Torrado, R. (2014) · BMC Genomics · doi:10.1186/1471-2164-15-432 · Cited by 41 · see citing works
Valadkhan, S., & Manley, J. L. (2000) · RNA · doi:10.1017/s1355838200992197 · Cited by 41 · see citing works
Functionally redundant interactions between U2 and U6 spliceosomal snRNAs.Preprint
Field, D. J., & Friesen, J. D. (1996) · Genes & Development · doi:10.1101/gad.10.4.489 · Cited by 41 · see citing works
Vidaver, R. M., Fortner, D. M., Loos-Austin, L. S., & Brow, D. A. (1999) · Genetics · doi:10.1093/genetics/153.3.1205 · Cited by 39 · see citing works
Self-citationStammler, S. N., Cao, S., Chen, S., & Giedroc, D. P. (2011) · RNA · doi:10.1261/rna.2816711 · Cited by 38 · see citing works
Shukla, G. C., & Padgett, R. A. (2001) · RNA · doi:10.1017/s1355838201000218 · Cited by 37 · see citing works
Liu, S., Mozaffari‐Jovin, S., Wollenhaupt, J., Santos, K. F., Theuser, M., Dunin-Horkawicz, S., Fabrizio, P., Bujnicki, J. M., Lührmann, R., & Wahl, M. C. (2015) · eLife · doi:10.7554/elife.07320 · Cited by 36 · see citing works
Venditti, V., Clos, L. J., Niccolai, N., & Butcher, S. E. (2009) · Journal of Molecular Biology · doi:10.1016/j.jmb.2009.07.003 · Cited by 36 · see citing works
Ryan, D. E., Stevens, S. W., & Abelson, J. N. (2002) · RNA · doi:10.1017/s1355838202026092 · Cited by 35 · see citing works
Gururajan, R., & Weeks, D. L. (1997) · Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression · doi:10.1016/s0167-4781(96)00155-8 · Cited by 35 · see citing works
Structure of the U6 RNA intramolecular stem–loop harboring an SP-phosphorothioate modification
Reiter, N. J., Nikstad, L. J., Allmann, A. M., Johnson, R. J., & Butcher, S. E. (2003) · RNA · doi:10.1261/rna.2199103 · Cited by 34 · see citing works
Cao, Y., & Woodson, S. A. (1998) · RNA · doi:10.1017/s1355838298980621 · Cited by 33 · see citing works
Johnson, T., & Abelson, J. N. (2001) · Genes & Development · doi:10.1101/gad.895601 · Cited by 32 · see citing works
A dynamic bulge in the U6 RNA internal stem–loop functions in spliceosome assembly and activation
McManus, C. J., Schwartz, M. L., Butcher, S. E., & Brow, D. A. (2007) · RNA · doi:10.1261/rna.699907 · Cited by 31 · see citing works
Self-citationSmall nuclear RNAs and pre-mRNA splicing
Newman, A. J. (1994) · Current Opinion in Cell Biology · doi:10.1016/0955-0674(94)90027-2 · Cited by 31 · see citing works
Karaduman, R., Dube, P., Stark, H., Fabrizio, P., Kastner, B., & Lührmann, R. (2008) · RNA · doi:10.1261/rna.1369808 · Cited by 30 · see citing works
Structural Basis for a Lethal Mutation in U6 RNA,
Sashital, D. G., Allmann, A. M., Doren, S. R. V., & Butcher, S. E. (2003) · Biochemistry · doi:10.1021/bi027137h · Cited by 30 · see citing works
Surprises in the 3′‐end: ‘U’ can decide too!
Viegas, S. C., Silva, I. J., Apura, P., Matos, R. G., & Arraiano, C. M. (2015) · FEBS Journal · doi:10.1111/febs.13377 · Cited by 29 · see citing works
Martin-Tumasz, S., Reiter, N. J., Brow, D. A., & Butcher, S. E. (2010) · RNA · doi:10.1261/rna.1913310 · Cited by 29 · see citing works
Self-citationCrystal structure of Cwc2 reveals a novel architecture of a multipartite RNA‐binding protein
Schmitzová, J., Rasche, N., Dybkov, O., Kramer, K., Fabrizio, P., Urlaub, H., Lührmann, R., & Peña, V. (2012) · The EMBO Journal · doi:10.1038/emboj.2012.58 · Cited by 29 · see citing works
García‐Ríos, E., Querol, A., & Guillamón, J. M. (2016) · Journal of Proteomics · doi:10.1016/j.jprot.2016.06.023 · Cited by 29 · see citing works
Mechanisms of Yeast Adaptation to Wine Fermentations
García‐Ríos, E., & Guillamón, J. M. (2019) · Progress in molecular and subcellular biology · doi:10.1007/978-3-030-13035-0_2 · Cited by 28 · see citing works
Substrate-assisted mechanism of RNP disruption by the spliceosomal Brr2 RNA helicase
Theuser, M., Höbartner, C., Wahl, M. C., & Santos, K. F. (2016) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1524616113 · Cited by 28 · see citing works
Measuring the dynamic surface accessibility of RNA with the small paramagnetic molecule TEMPOL
Venditti, V., Niccolai, N., & Butcher, S. E. (2007) · Nucleic Acids Research · doi:10.1093/nar/gkm1062 · Cited by 28 · see citing works
Kwan, S. S., & Brow, D. A. (2005) · RNA · doi:10.1261/rna.2010905 · Cited by 28 · see citing works
Self-citationXu, G., Wieland, B., & Bindereif, A. (1994) · Molecular and Cellular Biology · doi:10.1128/mcb.14.7.4565 · Cited by 28 · see citing works
The conserved central domain of yeast U6 snRNA: Importance of U2-U6 helix Ia in spliceosome assembly
Ryan, D. E., & Abelson, J. N. (2002) · RNA · doi:10.1017/s1355838202025013 · Cited by 26 · see citing works
Mutation in the U2 snRNA influences exon interactions of U5 snRNA loop 1 during pre‐mRNA splicing
McGrail, J. C., Tatum, E. M., & O’Keefe, R. T. (2006) · The EMBO Journal · doi:10.1038/sj.emboj.7601258 · Cited by 25 · see citing works
Intrinsic metal binding by a spliceosomal RNA
Valadkhan, S., & Manley, J. L. (2002) · Nature Structural Biology · doi:10.1038/nsb0702-498 · Cited by 25 · see citing works
Walker, S. C., & Avis, J. M. (2004) · Journal of Molecular Biology · doi:10.1016/j.jmb.2004.05.076 · Cited by 25 · see citing works
Structural requirements for protein-catalyzed annealing of U4 and U6 RNAs during di-snRNP assembly
Didychuk, A. L., Montemayor, E., Brow, D. A., & Butcher, S. E. (2015) · Nucleic Acids Research · doi:10.1093/nar/gkv1374 · Cited by 24 · see citing works
Self-citationChen, X. S., White, W. T. J., Collins, L. J., & Penny, D. (2008) · PLoS ONE · doi:10.1371/journal.pone.0003106 · Cited by 23 · see citing works
Jakab, G., Mougin, A., Kis, M., Pollák, T., Antal, M., Branlant, C., & Solymosy, F. (1997) · Biochimie · doi:10.1016/s0300-9084(97)86148-2 · Cited by 23 · see citing works
Kaiser, M. W., & Brow, D. A. (1995) · Journal of Biological Chemistry · doi:10.1074/jbc.270.19.11398 · Cited by 23 · see citing works
Self-citationIdentification and analysis of the u6 small nuclear RNA gene from Entamoeba histolytica
Miranda-CasoLuengo, R., Salgado, L. M., Sánchez‐López, R., Alagon, A. C., & Lizardi, P. M. (1996) · Gene · doi:10.1016/s0378-1119(96)00397-6 · Cited by 20 · see citing works
Widespread temperature sensitivity and tRNA decay due to mutations in a yeast tRNA
Payea, M. J., Sloma, M. F., Kon, Y., Young, D. L., Guy, M. P., Zhang, X., Zoysa, T. D., Fields, S., Mathews, D. H., & Phizicky, E. M. (2017) · RNA · doi:10.1261/rna.064642.117 · Cited by 19 · see citing works
Directing transcription of an RNA polymerase III gene via GAL4 sites.
Marsolier‐Kergoat, M., Chaussivert, N., Lefebvre, O., Conesa, C., Werner, M., & Sentenac, A. (1994) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.91.25.11938 · Cited by 19 · see citing works
Salvadó, Z., Ramos-Alonso, L., Tronchoni, J., Penacho, V., García‐Ríos, E., Morales, P., Gonzalez, R., & Guillamón, J. M. (2016) · International Journal of Food Microbiology · doi:10.1016/j.ijfoodmicro.2016.07.010 · Cited by 18 · see citing works
Secondary structure of U6 small nuclear RNA: implications for spliceosome assembly
Dunn, E. A., & Rader, S. D. (2010) · Biochemical Society Transactions · doi:10.1042/bst0381099 · Cited by 18 · see citing works
A Drosophila model of oral peptide therapeutics for adult intestinal stem cell tumors
Bajpai, A., Ahmad, Q. T., Tang, H.-W., Manzar, N., Singh, V. P., Thakur, A. K., Ateeq, B., Perrimon, N., & Sinha, P. (2020) · Disease Models & Mechanisms · doi:10.1242/dmm.044420 · Cited by 17 · see citing works
Eysmont, K., Matylla-Kulińska, K., Jaskulska, A., Magnus, M., & Konarska, M. M. (2019) · Molecular Cell · doi:10.1016/j.molcel.2019.05.018 · Cited by 16 · see citing works
Kwan, S. S., Gerlach, V. L., & Brow, D. A. (2000) · RNA · doi:10.1017/s1355838200991325 · Cited by 15 · see citing works
Self-citationWolff, T., & Bindereif, A. (1995) · Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression · doi:10.1016/0167-4781(95)00085-u · Cited by 12 · see citing works
Spliceosome assembly in the absence of stable U4/U6 RNA pairing
Burke, J. E., Butcher, S. E., & Brow, D. A. (2015) · RNA · doi:10.1261/rna.048421.114 · Cited by 10 · see citing works
Self-citationKitagawa, J. (2003) · Nucleic Acids Research · doi:10.1093/nar/gkg288 · Cited by 10 · see citing works
Thermodynamic examination of pH and magnesium effect on U6 RNA internal loop
O'Connell, A. A., Hanson, J. A., McCaskill, D. C., Moore, E. T., Lewis, D. C., & Grover, N. (2019) · RNA · doi:10.1261/rna.070466.119 · Cited by 8 · see citing works
Kaiser, M. W. (2004) · Nucleic Acids Research · doi:10.1093/nar/gkh769 · Cited by 8 · see citing works
Xu, G., Wieland, B., & Bindereif, A. (1994) · Molecular and Cellular Biology · doi:10.1128/mcb.14.7.4565-4570.1994 · Cited by 8 · see citing works
Site‐specific deoxynucleotide substitutions in yeast U6 snRNA block splicing of pre‐mRNA in vitro
Kim, C. H., Ryan, D. E., Marciniec, T., & Abelson, J. (1997) · The EMBO Journal · doi:10.1093/emboj/16.8.2119 · Cited by 8 · see citing works
RetractedA UV-crosslinkable interaction in human U6 snRNA
SUN, J.-S., Valadkhan, S., & Manley, J. L. (1998) · RNA · doi:10.1017/s1355838298980141 · Cited by 7 · see citing works
Sahasrabudhe, P. V., Sun, J., & Gmeiner, W. H. (1997) · Journal of Biomolecular Structure and Dynamics · doi:10.1080/07391102.1997.10508157 · Cited by 5 · see citing works
Pre-mRNA Splicing and the Spliceosome: Assembly, Catalysis, and Fidelity
Dunn, E. A., & Rader, S. D. (2014) · Springer eBooks · doi:10.1007/978-3-319-05687-6_2 · Cited by 4 · see citing works
Vaughn, J. C., Ghosh, S., & Chen, J. (2013) · Open Journal of Animal Sciences · doi:10.4236/ojas.2013.34a2005 · Cited by 2 · see citing works
Wang, X., Guo, J., Li, J., & Krebber, H. (2025) · Nucleic Acids Research · doi:10.1093/nar/gkaf1500 · Cited by 1 · see citing works
VGLL1 drives therapy resistance in estrogen receptor positive breast cancerPreprint
Gemma, C., Lai, C., Singh, A. K., Belfiore, A., Portman, N., Milioli, H. H., Periyasamy, M., Abuelmaaty, S., Nicholls, A. J., Davies, C. M., et al. (2020) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2020.11.29.402842 · Cited by 1 · see citing works
Dunn, W. S. C. (2011) · doi:10.24124/2011/bpgub780 · Cited by 1 · see citing works
U6 snRNA secondary structure in free U6 snRNPs.
Dunn, E. A. (2010) · doi:10.24124/2010/bpgub646 · Cited by 1 · see citing works
Gessler, L., Siudzińska, A., Prószyński, T. J., Sandri, M., von Eyß, B., & Hashemolhosseini, S. (2024) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2024.06.11.598443 · Cited by 1 · see citing works
Vaidya, V. C., & Vijayraghavan, U. (1998) · Journal of Genetics · doi:10.1007/bf02966594
Spliceosome assembly and rearrangements : understanding how snRNPs are built and helicases function
Lardelli, R. M. (2010) · Texas ScholarWorks (Texas Digital Library)
Dual nuclear roles of an essential scavenger decapping enzyme
Shen, V. (2010) · Rutgers University Community Repository (Rutgers University) · doi:10.7282/t3df6qzt
- Authors
- Fortner, D. M., Troy, R. G., & Brow, D. A.
- Affiliations
- University of Wisconsin System (US)
- ORCID
- Brow, D A
- Venue
- Genes & Development
- Publisher
- Cold Spring Harbor Laboratory
- Published
- 1994-01
- Volume
- 8
- Issue
- 2
- Pages
- 221-233
- ISSN
- 0890-9369 (ISSN-L)
- 1549-5477
- OpenAlex
- W2104728352
- Cited by
- 110
- References
- 62
- Citations by year
- 2026: 1 · 2025: 3 · 2024: 1 · 2020: 2 · 2019: 4 · 2018: 2 · 2017: 3 · 2016: 3 · 2015: 6 · 2014: 4 · 2013: 2 · 2012: 4
- Type
- posted content
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Genes & Development (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Chicago Community Trust
- Searle Scholars Program
- National Institutes of Health
- National Institute of General Medical Sciences (GM 07215, T32GM007215)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Research and Splicing
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA and protein synthesis mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA regulation and disease
- Keywords
- U6 small nuclear RNA, conformational switching, pre-mRNA splicing, spliceosome, U4 snRNA, U2 snRNA, Saccharomyces cerevisiae, RNA base pairing
- MeSH terms
- Base Sequence, Molecular Sequence Data, Mutation, Nucleic Acid Conformation, Phenotype, RNA Splicing, RNA, Fungal, RNA, Messenger, RNA, Small Nuclear, Saccharomyces cerevisiae, Suppression, Genetic
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review