The DOI matches its Crossref record; 46 of the 46 references it cites were found in a registry or an index.
Key events of pancreas formation are triggered in gut endoderm by ectopic expression of pancreatic regulatory genes
Open accessAbstract
The mechanisms by which the epithelium of the digestive tract and its associated glands are specified are largely unknown. One clue is that several transcription factors are expressed in specific regions of the endoderm prior to and during organogenesis. Pdx-1, for example, is expressed in the duodenum and pancreas and Pdx-1 inactivation results in an arrest of pancreatic development after buds formation. Similarly, ngn3 is transiently expressed in the developing pancreas and a knockout results in the absence of endocrine cells. This paper focuses on the question of whether these and other transcription factors, known to be necessary for pancreatic development, are also sufficient to drive a program of pancreatic organogenesis. Using in ovo electroporation of chick embryos, we show that ectopic expression of Pdx-1 or ngn3 causes cells to bud out of the epithelium like pancreatic progenitors. The Pdx-1-expressing cells extinguish markers for other nonpancreatic regions of the endoderm and initiate, but do not complete, pancreatic cytodifferentiation. Ectopic expression of ngn3 is sufficient to turn endodermal cells of any region into endocrine cells that form islets expressing glucagon and somatostatin in the mesenchyme. The results suggest that simple gene combinations could be used in stem cells to achieve specific endodermal tissue differentiation.
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Cites
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Cited by
Developmental biology of the pancreas: A comprehensive review
Gittes, G. K. (2009) · Developmental Biology · doi:10.1016/j.ydbio.2008.10.024 · Cited by 451
Hex homeobox gene-dependent tissue positioning is required for organogenesis of the ventral pancreas
Bort, R., Martinez-Barbera, J. P., Beddington, R. S. P., & Zaret, K. S. (2004) · Development · doi:10.1242/dev.00965 · Cited by 267
Molecular regulation of pancreatic β-cell mass development, maintenance, and expansion
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Direct lineage conversions: unnatural but useful?
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Global expression analysis of gene regulatory pathways during endocrine pancreatic development
Gu, G., Wells, J. M., Dombkowski, D., Preffer, F., Aronow, B., & Melton, D. A. (2004) · Development · doi:10.1242/dev.00921 · Cited by 229
Self-citation
Metadata
- Authors
- Grapin-Botton, A., Majithia, A. R., & Melton, D. A.
- Venue
- Genes & Development
- Publisher
- Cold Spring Harbor Laboratory
- Published
- 2001-02-15
- Volume
- 15
- Issue
- 4
- Pages
- 444-454
- ISSN
- 0890-9369 (ISSN-L)
- 1549-5477
- OpenAlex
- W1968558135
- Cited by
- 296
- References
- 46
- Type
- posted content
- Language
- en
- Open access
- diamond
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- Grapin-Botton, A., Majithia, A. R., & Melton, D. A. (2001). Key events of pancreas formation are triggered in gut endoderm by ectopic expression of pancreatic regulatory genes. Genes & Development. https://doi.org/10.1101/gad.846001
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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