The DOI matches its Crossref record; 11 of the 11 references it cites were found in a registry or an index.
SMC1 is a downstream effector in the ATM/NBS1 branch of the human S-phase checkpoint
Open accessAbstract
Structural maintenance of chromosomes (SMC) proteins (SMC1, SMC3) are evolutionarily conserved chromosomal proteins that are components of the cohesin complex, necessary for sister chromatid cohesion. These proteins may also function in DNA repair. Here we report that SMC1 is a component of the DNA damage response network that functions as an effector in the ATM/NBS1-dependent S-phase checkpoint pathway. SMC1 associates with BRCA1 and is phosphorylated in response to IR in an ATM- and NBS1-dependent manner. Using mass spectrometry, we established that ATM phosphorylates S957 and S966 of SMC1 in vivo. Phosphorylation of S957 and/or S966 of SMC1 is required for activation of the S-phase checkpoint in response to IR. We also discovered that the phosphorylation of NBS1 by ATM is required for the phosphorylation of SMC1, establishing the role of NBS1 as an adaptor in the ATM/NBS1/SMC1 pathway. The ATM/CHK2/CDC25A pathway is also involved in the S-phase checkpoint activation, but this pathway is intact in NBS cells. Our results indicate that the ATM/NBS1/SMC1 pathway is a separate branch of the S-phase checkpoint pathway, distinct from the ATM/CHK2/CDC25A branch. Therefore, this work establishes the ATM/NBS1/SMC1 branch, and provides a molecular basis for the S-phase checkpoint defect in NBS cells.
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Cites
The DNA damage response: putting checkpoints in perspective
Zhou, B.-B. S., & Elledge, S. J. (2000) · Nature · doi:10.1038/35044005 · Cited by 3,213
Wang, Y., Cortez, D., Yazdi, P., Neff, N., Elledge, S. J., & Qin, J. (2000) · Genes & Development · doi:10.1101/gad.14.8.927 · Cited by 1,221 · cited 6×
“(Wang et al. 2000).”
MethodInfluentialSelf-citationThe ATM-Chk2-Cdc25A checkpoint pathway guards against radioresistant DNA synthesis
Falck, J., Mailand, N., Syljuåsen, R. G., Bartek, J., & Lukas, J. (2001) · Nature · doi:10.1038/35071124 · Cited by 1,085
Substrate Specificities and Identification of Putative Substrates of ATM Kinase Family Members
Kim, S.-T., Lim, D.-S., Canman, C. E., & Kastan, M. B. (1999) · Journal of Biological Chemistry · doi:10.1074/jbc.274.53.37538 · Cited by 810
Hashizume, R., Fukuda, M., Maeda, I., Nishikawa, H., Oyake, D., Yabuki, Y., Ogata, H., & Ohta, T. (2001) · Journal of Biological Chemistry · doi:10.1074/jbc.c000881200 · Cited by 634
In situ visualization of DNA double-strand break repair in human fibroblasts
Nelms, B. E., Maser, R. S., MacKay, J. F., Lagally, M. G., & Petrini, J. H. J. (1998) · Science · doi:10.1126/science.280.5363.590 · Cited by 500
DNA Damage-Dependent Nuclear Dynamics of the Mre11 Complex
Mirzoeva, O. K., & Petrini, J. H. J. (2001) · Molecular and Cellular Biology · doi:10.1128/mcb.21.1.281-288.2001 · Cited by 387
The yeast Xrs2 complex functions in S phase checkpoint regulation
D'Amours, D., & Jackson, S. P. (2001) · Genes & Development · doi:10.1101/gad.208701 · Cited by 228
Chk2 Activation Dependence on Nbs1 after DNA Damage
Buscemi, G., Savio, C., Zannini, L., Miccichè, F., Masnada, D., Nakanishi, M., Tauchi, H., Komatsu, K., Mizutani, S., Khanna, K., et al. (2001) · Molecular and Cellular Biology · doi:10.1128/mcb.21.15.5214-5222.2001 · Cited by 201
Utilization of Oriented Peptide Libraries to Identify Substrate Motifs Selected by ATM
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Cited by
DNA damage activates ATM through intermolecular autophosphorylation and dimer dissociation
Bakkenist, C. J., & Kastan, M. B. (2003) · Nature · doi:10.1038/nature01368 · Cited by 3,466
Molecular Mechanisms of Mammalian DNA Repair and the DNA Damage Checkpoints
Sancar, A., Lindsey-Boltz, L. A., Ünsal-Kaçmaz, K., & Linn, S. (2004) · Annual Review of Biochemistry · doi:10.1146/annurev.biochem.73.011303.073723 · Cited by 3,317
ATM and ATR Substrate Analysis Reveals Extensive Protein Networks Responsive to DNA Damage
Matsuoka, S., Ballif, B. A., Smogorzewska, A., McDonald, E. R., Hurov, K. E., Luo, J., Bakalarski, C. E., Zhao, Z., Solimini, N., Lerenthal, Y., et al. (2007) · Science · doi:10.1126/science.1140321 · Cited by 3,075
Cell-cycle checkpoints and cancer
Kastan, M. B., & Bartek, J. (2004) · Nature · doi:10.1038/nature03097 · Cited by 2,836
ATM and related protein kinases: safeguarding genome integrity
Shiloh, Y. (2003) · Nature Reviews Cancer · doi:10.1038/nrc1011 · Cited by 2,577
Metadata
- Authors
- Yazdi, P. T., Wang, Y., Zhao, S., Patel, N., Lee, E. Y.-H. P., & Qin, J.
- Venue
- Genes & Development
- Publisher
- Cold Spring Harbor Laboratory
- Published
- 2002-03-01
- Volume
- 16
- Issue
- 5
- Pages
- 571-582
- ISSN
- 0890-9369 (ISSN-L)
- 1549-5477
- OpenAlex
- W2118609150
- Cited by
- 506
- References
- 11
- Type
- posted content
- Language
- en
- Open access
- diamond
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- CoChat. (2026). Citation check: SMC1 is a downstream effector in the ATM/NBS1 branch of the human S-phase checkpoint. https://app.cochat.ai/papers/10.1101/gad.970702
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- Yazdi, P. T., Wang, Y., Zhao, S., Patel, N., Lee, E. Y.-H. P., & Qin, J. (2002). SMC1 is a downstream effector in the ATM/NBS1 branch of the human S-phase checkpoint. Genes & Development. https://doi.org/10.1101/gad.970702
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