Cited by 500DNA Repair MechanismsEffects of Radiation ExposurePARP inhibition in cancer therapy

In situ visualization of DNA double-strand break repair in human fibroblasts

Nelms, Benjamin E., Maser, Richard S., MacKay, James F., Lagally, Max G., Petrini, John H. J.·1998·Science

Abstract

A method was developed to examine DNA repair within the intact cell. Ultrasoft x-rays were used to induce DNA double-strand breaks (DSBs) in defined subnuclear volumes of human fibroblasts and DNA repair was visualized at those sites. The DSBs remained in a fixed position during the initial stages of DNA repair, and the DSB repair protein hMre11 migrated to the sites of damage within 30 minutes. In contrast, hRad51, a human RecA homolog, did not localize at sites of DNA damage, a finding consistent with the distinct roles of these proteins in DNA repair.

Integrity signals

Registry

Crossref · 10.1126/science.280.5363.590

Cites

39 of 39 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Cites

Flagged first, then by citations

Cited by

Sorted by citations

Authors
Nelms, B. E., Maser, R. S., MacKay, J. F., Lagally, M. G., & Petrini, J. H. J.
Affiliations
University of Wisconsin System (US)
Corresponding author
  • Nelms, Benjamin E.
  • Maser, Richard S.
  • Petrini, John H. J.
Venue
Science
Published
1998-04-24
Volume
280
Issue
5363
Pages
590-592
ISSN
  • 0036-8075 (ISSN-L)
  • 1095-9203
OpenAlex
W2054889035
Cited by
500
References
38
Citations by year
2025: 1 · 2024: 2 · 2023: 6 · 2022: 3 · 2021: 3 · 2020: 10 · 2019: 3 · 2018: 11 · 2017: 7 · 2016: 13 · 2015: 5 · 2014: 21 · 2013: 16 · 2012: 13
FWCI
12.98
Type
journal article
Language
en
Open access
closed
Locations
  • Science (publishedVersion)
  • PubMed (publishedVersion)
Funders
National Institute of General Medical Sciences (T32GM008349, T32GM007133)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA Repair Mechanisms
  • Health Sciences › Medicine › Radiology, Nuclear Medicine and Imaging › Effects of Radiation Exposure
  • Health Sciences › Medicine › Oncology › PARP inhibition in cancer therapy
Keywords
DNA double-strand break repair, human fibroblasts, in situ visualization, ultrasoft X-rays, RecA homolog, subnuclear localization, DNA damage response, protein localization
MeSH terms
Bromodeoxyuridine, Cell Line, Cell Nucleus, DNA, DNA Damage, DNA Repair, DNA-Binding Proteins, Fibroblasts, Fluorescent Antibody Technique, Humans, Fluorescein-5-isothiocyanate, Microscopy, Confocal, Rad51 Recombinase

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review