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Specific down-modulation of Notch1 signaling in cervical cancer cells is required for sustained HPV-E6/E7 expression and late steps of malignant transformation

Talora, Claudio, Sgroi, Dennis C., Crum, Christopher P., Dotto, G. Paolo·2002·Genes & Development
DOI 10.1101/gad.988902
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Abstract

The Notch family of cell surface receptors plays a key role in cell-fate determination and differentiation, functioning in a cell- and context-specific manner. In mammalian cells, Notch activation is generally thought to maintain stem cell potential and inhibit differentiation, thereby promoting carcinogenesis. However, in other contexts such as primary epithelial cells (keratinocytes), increased Notch activity causes exit from the cell cycle and/or commitment to differentiation. We now report that expression of the endogenous Notch1 gene is markedly reduced in a panel of cervical carcinoma cells whereas expression of Notch2 remains elevated, and Notch1 expression is similarly reduced or absent in invasive cervical cancers. Conversely, expression of activated Notch1 causes strong growth inhibition of HPV-positive, but not HPV-negative, cervical carcinoma cells, but exerts no such effects on other epithelial tumor cells. Increased Notch1 signaling, but not Notch2, causes a dramatic down-modulation of HPV-driven transcription of the E6/E7 viral genes, through suppression of AP-1 activity by up-regulation of the Fra-1 family member and decreased c-Fos expression. Thus, Notch1 exerts specific protective effects against HPV-induced transformation through suppression of E6/E7 expression, and down-modulation of Notch1 expression is likely to play an important role in late stages of HPV-induced carcinogenesis.

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Metadata

Authors
Talora, C., Sgroi, D. C., Crum, C. P., & Dotto, G. P.
Venue
Genes & Development
Publisher
Cold Spring Harbor Laboratory
Published
2002-09-01
Volume
16
Issue
17
Pages
2252-2263
ISSN
  • 0890-9369
  • 1549-5477
DOI
10.1101/gad.988902
Cited by
206
References
1
Language
en

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