The DOI matches its Crossref record; 4 of the 4 references it cites were found in a registry or an index.
Genome structure of a Saccharomyces cerevisiae strain widely used in bioethanol production
Open accessAbstract
Bioethanol is a biofuel produced mainly from the fermentation of carbohydrates derived from agricultural feedstocks by the yeast Saccharomyces cerevisiae. One of the most widely adopted strains is PE-2, a heterothallic diploid naturally adapted to the sugar cane fermentation process used in Brazil. Here we report the molecular genetic analysis of a PE-2 derived diploid (JAY270), and the complete genome sequence of a haploid derivative (JAY291). The JAY270 genome is highly heterozygous (∼2 SNPs/kb) and has several structural polymorphisms between homologous chromosomes. These chromosomal rearrangements are confined to the peripheral regions of the chromosomes, with breakpoints within repetitive DNA sequences. Despite its complex karyotype, this diploid, when sporulated, had a high frequency of viable spores. Hybrid diploids formed by outcrossing with the laboratory strain S288c also displayed good spore viability. Thus, the rearrangements that exist near the ends of chromosomes do not impair meiosis, as they do not span regions that contain essential genes. This observation is consistent with a model in which the peripheral regions of chromosomes represent plastic domains of the genome that are free to recombine ectopically and experiment with alternative structures. We also explored features of the JAY270 and JAY291 genomes that help explain their high adaptation to industrial environments, exhibiting desirable phenotypes such as high ethanol and cell mass production and high temperature and oxidative stress tolerance. The genomic manipulation of such strains could enable the creation of a new generation of industrial organisms, ideally suited for use as delivery vehicles for future bioenergy technologies.
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Versatile and open software for comparing large genomes
Kurtz, S., Phillippy, A., Delcher, A. L., Smoot, M., Shumway, M., Antonescu, C., & Salzberg, S. L. (2004) · Genome Biology · doi:10.1186/gb-2004-5-2-r12 · Cited by 5,254
Ethanol for a Sustainable Energy Future
Goldemberg, J. (2007) · Science · doi:10.1126/science.1137013 · Cited by 1,423
Formation of new chromosomes as a virulence mechanism in yeast Candida glabrata
Poláková, S., Blume, C., Zárate, J. Á., Mentel, M., Jørck-Ramberg, D., Stenderup, J., & Piškur, J. (2009) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0809793106 · Cited by 206
Tamura, K.-I., Gu, Y., Wang, Q., Yamada, T., Ito, K., & Shimoi, H. (2004) · Journal of Bioscience and Bioengineering · doi:10.1016/s1389-1723(04)00260-9 · Cited by 46
Cited by
Scientific challenges of bioethanol production in Brazil
Amorim, H. V., Lopes, M. L., de Castro Oliveira, J. V., Buckeridge, M. S., & Goldman, G. H. (2011) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-011-3437-6 · Cited by 396
The origin and adaptive evolution of domesticated populations of yeast from Far East Asia
Duan, S.-F., Han, P.-J., Wang, Q.-M., Liu, W.-Q., Shi, J.-Y., Li, K., Zhang, X.-L., & Bai, F.-Y. (2018) · Nature Communications · doi:10.1038/s41467-018-05106-7 · Cited by 244
Oud, B., Maris, A. J. A., Daran, J.-M., & Pronk, J. T. (2012) · FEMS Yeast Research · doi:10.1111/j.1567-1364.2011.00776.x · Cited by 82
“…(classical Sanger sequencing; Roche 454 or new Pacific Biosystems) and mate-paired libraries of several insert sizes (ranging from 400 bp to 10 kbp) (Table 1) to close gaps in the assembled sequence and to obtain reliable assemblies of repetitive sequences (Argueso et al., 2009; Novo et al., 2009).”
Background
Metadata
- Authors
- Argueso, J. L., Carazzolle, M. F., Mieczkowski, P. A., Duarte, F. M., Netto, O. V. C., Missawa, S. K., Galzerani, F., Costa, G. G. L., Vidal, R. O., Noronha, M. F., Dominska, M., Andrietta, M. G. S., Andrietta, S. R., Cunha, A. F., Gomes, L. H., Tavares, F. C. A., Alcarde, A. R., Dietrich, F. S., McCusker, J. H., Petes, T. D., & Pereira, G. A. G.
- Venue
- Genome Research
- Publisher
- Cold Spring Harbor Laboratory
- Published
- 2009-10-07
- Volume
- 19
- Issue
- 12
- Pages
- 2258-2270
- ISSN
- 1088-9051 (ISSN-L)
- OpenAlex
- W4301828141
- Cited by
- 289
- References
- 4
- Type
- posted content
- Language
- en
- Open access
- hybrid
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- Argueso, J. L., Carazzolle, M. F., Mieczkowski, P. A., Duarte, F. M., Netto, O. V. C., Missawa, S. K., Galzerani, F., Costa, G. G. L., Vidal, R. O., Noronha, M. F., Dominska, M., Andrietta, M. G. S., Andrietta, S. R., Cunha, A. F., Gomes, L. H., Tavares, F. C. A., Alcarde, A. R., Dietrich, F. S., McCusker, J. H., . . . Pereira, G. A. G. (2009). Genome structure of a Saccharomyces cerevisiae strain widely used in bioethanol production. Genome Research. https://doi.org/10.1101/gr.091777.109
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
