Cited by 46Fungal and yeast genetics researchYeasts and Rust Fungi StudiesBacterial Genetics and Biotechnology

A hap1 mutation in a laboratory strain of Saccharomyces cerevisiae results in decreased expression of ergosterol-related genes and cellular ergosterol content compared to sake yeast

Tamura, Ken-Ichi, Gu, Yongqing, Wang, Qian, Yamada, Tasuku, Ito, Kiyoshi, Shimoi, Hitoshi·2004·Journal of Bioscience and Bioengineering

Abstract

DNA microarray and Northern blot analysis revealed that a sake yeast strain Kyokai no. 7 (K7) showed higher expression of genes encoding proteins involved in ergosterol biosynthesis than a laboratory yeast strain X2180. We hypothesized that these differences in expression levels were caused by a defect of a transcriptional factor Hap1, because strain X2180 contained a Ty1 insertion mutation in the HAP1 gene. To confirm this, we constructed a strain X2180 derivative (strain HX) that contained the wild-type HAP1 genes originating from strain K7. The expression levels of ergosterol-related genes and cellular ergosterol content in strain HX were higher than those in strain X2180 and were almost comparable to those in strain K7. These results suggest that the differences in the expression levels of ergosterol-related genes and ergosterol content between strains K7 and X2180 were largely caused by the hap1 mutation in strain X2180. Involvement of the mutated Hap1 in the differential gene expression between strain K7 and strain X2180 was further confirmed by a lacZ reporter assay of HMG1, one of the Hap1-regulated genes. We also revealed that the HMG1 promoter region between -500 and -376 was important in the transcriptional activation by Hap1.

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Crossref · 10.1016/s1389-1723(04)00260-9

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  • Yeast strains with a HAP1-inserted transposon have less cellular ergosterol content.

    “Consistent with these observations, yeast strains with a HAP1-inserted transposon have less cellular ergosterol content [80].”
  • The heme-dependent transcription factor Hap1 is also necessary for the expression of genes involved in ergosterol biosynthesis.

    “The heme-dependent transcription factor Hap1 is also necessary for the expression of genes involved in ergosterol biosynthesis (Figure 3A) [75,79,80,81,82].”

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Authors
Tamura, K.-I., Gu, Y., Wang, Q., Yamada, T., Ito, K., & Shimoi, H.
Affiliations
  • Takara Shuzo Co., Ltd. (Japan) (JP)
  • Shihezi University (CN)
  • Hiroshima University (JP)
  • KIKU-Masamune SAKE Brewing Co., Ltd. (Japan) (JP)
Corresponding author
Shimoi, Hitoshi
Venue
Journal of Bioscience and Bioengineering
Publisher
Elsevier BV
Published
2004-01
Volume
98
Issue
3
Pages
159-166
ISSN
  • 1347-4421 (ISSN-L)
  • 1389-1723
OpenAlex
W1982988838
Cited by
46
References
30
Citations by year
2026: 1 · 2024: 3 · 2023: 2 · 2022: 2 · 2021: 2 · 2020: 2 · 2019: 2 · 2018: 1 · 2017: 1 · 2014: 3 · 2013: 1 · 2012: 3
FWCI
1.18
Type
journal article
Language
en
Open access
closed
Locations
  • Journal of Bioscience and Bioengineering (publishedVersion)
  • PubMed (publishedVersion)
Funders
Japan International Cooperation Agency
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Yeasts and Rust Fungi Studies
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
Keywords
HAP1, ergosterol biosynthesis, gene expression regulation, transcriptional activation, sake yeast, Saccharomyces cerevisiae

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