A hap1 mutation in a laboratory strain of Saccharomyces cerevisiae results in decreased expression of ergosterol-related genes and cellular ergosterol content compared to sake yeast
Abstract
DNA microarray and Northern blot analysis revealed that a sake yeast strain Kyokai no. 7 (K7) showed higher expression of genes encoding proteins involved in ergosterol biosynthesis than a laboratory yeast strain X2180. We hypothesized that these differences in expression levels were caused by a defect of a transcriptional factor Hap1, because strain X2180 contained a Ty1 insertion mutation in the HAP1 gene. To confirm this, we constructed a strain X2180 derivative (strain HX) that contained the wild-type HAP1 genes originating from strain K7. The expression levels of ergosterol-related genes and cellular ergosterol content in strain HX were higher than those in strain X2180 and were almost comparable to those in strain K7. These results suggest that the differences in the expression levels of ergosterol-related genes and ergosterol content between strains K7 and X2180 were largely caused by the hap1 mutation in strain X2180. Involvement of the mutated Hap1 in the differential gene expression between strain K7 and strain X2180 was further confirmed by a lacZ reporter assay of HMG1, one of the Hap1-regulated genes. We also revealed that the HMG1 promoter region between -500 and -376 was important in the transcriptional activation by Hap1.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Yeast strains with a HAP1-inserted transposon have less cellular ergosterol content.
“Consistent with these observations, yeast strains with a HAP1-inserted transposon have less cellular ergosterol content [80].”
From Regulation of Ergosterol Biosynthesis in Saccharomyces cerevisiae (2020) · cites this paper as [80]
The heme-dependent transcription factor Hap1 is also necessary for the expression of genes involved in ergosterol biosynthesis.
“The heme-dependent transcription factor Hap1 is also necessary for the expression of genes involved in ergosterol biosynthesis (Figure 3A) [75,79,80,81,82].”
From Regulation of Ergosterol Biosynthesis in Saccharomyces cerevisiae (2020) · cites this paper as [80]
Cites
Transformation of intact yeast cells treated with alkali cations
Ito, H., Fukuda, Y., Murata, K., & Kimura, A. (1983) · Journal of Bacteriology · doi:10.1128/jb.153.1.163-168.1983 · Cited by 7,518 · see citing works
Jacob, H. (1988) · Bioelectrochemistry and Bioenergetics · doi:10.1016/0302-4598(88)80052-7 · Cited by 4,911 · see citing works
New heterologous modules for classical or PCR‐based gene disruptions in Saccharomyces cerevisiae
Wach, A., Brachat, A., Pöhlmann, R., & Philippsen, P. (1994) · Yeast · doi:10.1002/yea.320101310 · Cited by 2,532 · see citing works
Genetic Dissection of Transcriptional Regulation in Budding Yeast
Brem, R. B., Yvert, G., Clinton, R., & Kruglyak, L. (2002) · Science · doi:10.1126/science.1069516 · Cited by 1,392 · see citing works
[12] High-efficiency transformation of yeast by electroporation
Becker, D. M., & Guarente, L. (1991) · Methods in enzymology on CD-ROM/Methods in enzymology · doi:10.1016/0076-6879(91)94015-5 · Cited by 805 · see citing works
[27] Preparation of high molecular weight RNA
Köhrer, K., & Domdey, H. (1991) · Methods in enzymology on CD-ROM/Methods in enzymology · doi:10.1016/0076-6879(91)94030-g · Cited by 574 · see citing works
Burns, N. A., Grimwade, B. G., Ross‐Macdonald, P., Choi, E. Y., Finberg, K. E., Roeder, G. S., & Snyder, M. P. (1994) · Genes & Development · doi:10.1101/gad.8.9.1087 · Cited by 490 · see citing works
The nucleotide sequence of Saccharomyces cerevisiae chromosome VII
Tettelin, H., Carbone, M. L. A., Albermann, K., Albers, M., Arroyo, J., Backes, U., BARREIROS, T., Bertani, I., Bjourson, A. J., Brückner, M., et al. (1997) · Nature · doi:10.1038/387s081 · Cited by 298 · see citing works
Basson, M. E., Thorsness, M., & Rine, J. (1986) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.83.15.5563 · Cited by 251 · see citing works
Oxygen Sensing and the Transcriptional Regulation of Oxygen-Responsive Genes in Yeast
Kwast, K. E., Burke, P. V., & Poyton, R. Ο. (1998) · Journal of Experimental Biology · doi:10.1242/jeb.201.8.1177 · Cited by 249 · see citing works
Polakowski, T., Ståhl, U., & Lang, C. (1998) · Applied Microbiology and Biotechnology · doi:10.1007/s002530051138 · Cited by 239 · see citing works
Specific Sterols Required for the Internalization Step of Endocytosis in Yeast
Munn, A. L., Heese-Peck, A., Stevenson, B. J., Pichler, H., & Riezman, H. (1999) · Molecular Biology of the Cell · doi:10.1091/mbc.10.11.3943 · Cited by 165 · see citing works
Molecular cloning and characterization of the yeast gene for squalene synthetase.
Jennings, S., Tsay, Y. H., Fisch, T. M., & Robinson, G. W. (1991) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.88.14.6038 · Cited by 162 · see citing works
Ergosterol is required for the Sec18/ATP‐dependent priming step of homotypic vacuole fusion
Kato, M., & Wickner, W. T. (2001) · The EMBO Journal · doi:10.1093/emboj/20.15.4035 · Cited by 158 · see citing works
Gaisne, M., Bécam, A. ‐., Verdière, J., & Herbert, C. J. (1999) · Current Genetics · doi:10.1007/s002940050490 · Cited by 147 · see citing works
A microarray‐assisted screen for potential Hap1 and Rox1 target genes in Saccharomyces cerevisiae
Linde, J. J. M. T., & Steensma, H. Y. (2002) · Yeast · doi:10.1002/yea.879 · Cited by 123 · see citing works
Skaggs, B., F.Alexander, J., Pierson, C. A., Schweitzer, K. S., Chun, K. T., Koegel, C., Barbuch, R. J., & Bard, M. (1996) · Gene · doi:10.1016/0378-1119(95)00770-9 · Cited by 120 · see citing works
Thorsness, M. K., Schafer, W., D'Ari, L., & Rine, J. (1989) · Molecular and Cellular Biology · doi:10.1128/mcb.9.12.5702 · Cited by 88 · see citing works
Kennedy, M. A., Barbuch, R., & Bard, M. (1999) · Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression · doi:10.1016/s0167-4781(99)00035-4 · Cited by 85 · see citing works
Becerra, M., Lombardía‐Ferreira, L. J., Hauser, N. C., Hoheisel, J. D., Tizon, B., & Cerdán, M. E. (2002) · Molecular Microbiology · doi:10.1046/j.1365-2958.2002.02724.x · Cited by 85 · see citing works
The nucleotide sequence of Saccharomyces cerevisiae chromosome XII
Johnston, M., Hillier, L., Riles, L., Albermann, K., André, B., Ansorge, W., Beneš, V., Brückner, M., Delius, H., Dubois, E., et al. (1997) · Nature · doi:10.1038/387s087 · Cited by 82 · see citing works
Feedback regulation of 3-hydroxy-3-methylglutaryl coenzyme A reductase in Saccharomyces cerevisiae.
Dimster-Denk, D., Thorsness, M. K., & Rine, J. (1994) · Molecular Biology of the Cell · doi:10.1091/mbc.5.6.655 · Cited by 78 · see citing works
ERG10 from Saccharomyces cerevisiae encodes acetoacetyl-CoA thiolase.
Hiser, L. M., Basson, M. E., & Rine, J. (1994) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)31705-8 · Cited by 65 · see citing works
Shimoi, H., Sakamoto, K., Okuda, M., Atthi, R., Iwashita, K., & Ito, K. (2002) · Applied and Environmental Microbiology · doi:10.1128/aem.68.4.2018-2025.2002 · Cited by 64 · see citing works
Self-citationJensen-Pergakes, K., Guo, Z., Giattina, M., Sturley, S. L., & Bard, M. (2001) · Journal of Bacteriology · doi:10.1128/jb.183.17.4950-4957.2001 · Cited by 64 · see citing works
INOUE, T., Iefuji, H., Fujii, T., Hiroshi, S. O. G. A., & SATOH, K. (2000) · Bioscience Biotechnology and Biochemistry · doi:10.1271/bbb.64.229 · Cited by 63 · see citing works
Ha, N. (1996) · Nucleic Acids Research · doi:10.1093/nar/24.8.1453 · Cited by 50 · see citing works
Cloning and Analysis of the AWA1 Gene of a Nonfoaming Mutant of a Sake Yeast
Miyashita, K., Sakamoto, K., Kitagaki, H., Iwashita, K., Ito, K., & Shimoi, H. (2004) · Journal of Bioscience and Bioengineering · doi:10.1016/s1389-1723(04)70158-9 · Cited by 24 · see citing works
Self-citationAlcohol dehydrogenase I of sake yeast Saccharomyces cerevisiae Kyokai no. 7
Ohbuchi, K., Ishikawa, Y., Kanda, A., Hamachi, M., & NUNOKAWA, Y. (1996) · Journal of Fermentation and Bioengineering · doi:10.1016/0922-338x(96)87589-1 · Cited by 9 · see citing works
Biochemistry, cell biology and molecular biology of lipids ofSaccharomyces cerevisiae
Daum, G., Lees, N. D., Bard, M., & Dickson, R. (1998) · Yeast · doi:10.1002/(sici)1097-0061(199812)14:16
Flagged first, then by citations
Cited by
Sorted by citations
Regulation of Ergosterol Biosynthesis in Saccharomyces cerevisiae
Jordá, T., & Puig, S. (2020) · Genes · doi:10.3390/genes11070795 · Cited by 489 · see citing works
Genome structure of a Saccharomyces cerevisiae strain widely used in bioethanol productionPreprint
Argueso, J. L., Carazzolle, M. F., Mieczkowski, P. A., Duarte, F. M., Netto, O. V. C., Missawa, S. K., Galzerani, F., Costa, G. G., Vidal, R. O., Noronha, M. F., et al. (2009) · Genome Research · doi:10.1101/gr.091777.109 · Cited by 289 · see citing works
Harnessing yeast subcellular compartments for the production of plant terpenoids
Farhi, M., Marhevka, E., Masci, T., Marcos‐Hadad, E., Eyal, Y., Ovadis, M., Abeliovich, H., & Vainstein, A. (2011) · Metabolic Engineering · doi:10.1016/j.ymben.2011.05.001 · Cited by 241 · see citing works
Coordination of Lipid Metabolism in Membrane Biogenesis
Nohturfft, A., & Zhang, S. C. (2009) · Annual Review of Cell and Developmental Biology · doi:10.1146/annurev.cellbio.24.110707.175344 · Cited by 175 · see citing works
Evidence for widespread adaptive evolution of gene expression in budding yeast
Fraser, H. B., Moses, A. M., & Schadt, E. E. (2010) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0912245107 · Cited by 154 · see citing works
Whole-Genome Sequencing of Sake Yeast Saccharomyces cerevisiae Kyokai no. 7
Akao, T., Yashiro, I., Hosoyama, A., Kitagaki, H., Horikawa, H., Watanabe, D., Akada, R., Ando, Y., Harashima, S., Inoue, T., et al. (2011) · DNA Research · doi:10.1093/dnares/dsr029 · Cited by 157 · see citing works
Self-citationHickman, M. J., & Winston, F. (2007) · Molecular and Cellular Biology · doi:10.1128/mcb.00887-07 · Cited by 137 · see citing works
A Role for Sterol Levels in Oxygen Sensing in <i>Saccharomyces cerevisiae</i>
Davies, B. S. J., & Rine, J. (2006) · Genetics · doi:10.1534/genetics.106.059964 · Cited by 123 · see citing works
Genetic basis of individual differences in the response to small-molecule drugs in yeast
Perlstein, E. O., Ruderfer, D. M., Roberts, D. C. K., Schreiber, S. L., & Kruglyak, L. (2007) · Nature Genetics · doi:10.1038/ng1991 · Cited by 123 · see citing works
Matsushika, A., Goshima, T., & Hoshino, T. (2014) · Microbial Cell Factories · doi:10.1186/1475-2859-13-16 · Cited by 84 · see citing works
Effects of Culture Conditions on Ergosterol Biosynthesis bySaccharomyces cerevisiae
Shobayashi, M., MITSUEDA, S.-I., Ago, M., Fujii, T., Iwashita, K., & Iefuji, H. (2005) · Bioscience Biotechnology and Biochemistry · doi:10.1271/bbb.69.2381 · Cited by 71 · see citing works
Watanabe, M., Tamura, K., Magbanua, J. P., Takano, K., Kitamoto, K., Kitagaki, H., Akao, T., & Shimoi, H. (2007) · Journal of Bioscience and Bioengineering · doi:10.1263/jbb.104.163 · Cited by 65 · see citing works
Self-citationLewis, J. A., Broman, A. T., Will, J., & Gasch, A. P. (2014) · Genetics · doi:10.1534/genetics.114.167429 · Cited by 62 · see citing works
Identification and Characterization of a Novel Biotin Biosynthesis Gene inSaccharomyces cerevisiae
Wu, H., Ito, K., & Shimoi, H. (2005) · Applied and Environmental Microbiology · doi:10.1128/aem.71.11.6845-6855.2005 · Cited by 63 · see citing works
Self-citationSahana, G. R., Balasubramanian, B., Joseph, K. S., Pappuswamy, M., Liu, W.-C., Meyyazhagan, A., Kamyab, H., Chelliapan, S., & Joseph, B. V. (2024) · Process Biochemistry · doi:10.1016/j.procbio.2023.12.024 · Cited by 59 · see citing works
Endo, A., Nakamura, T., & Shima, J. (2009) · FEMS Microbiology Letters · doi:10.1111/j.1574-6968.2009.01733.x · Cited by 52 · see citing works
Ethanol stress stimulates the Ca2+-mediated calcineurin/Crz1 pathway in Saccharomyces cerevisiae
Araki, Y., Wu, H., Kitagaki, H., Akao, T., Takagi, H., & Shimoi, H. (2009) · Journal of Bioscience and Bioengineering · doi:10.1016/j.jbiosc.2008.09.005 · Cited by 46 · see citing works
Self-citationTranscriptional regulation of ergosterol biosynthesis genes in response to iron deficiency
Jordá, T., Barba‐Aliaga, M., Rozés, N., Alepuz, P. M., Martínez‐Pastor, M. T., & Puig, S. (2022) · Environmental Microbiology · doi:10.1111/1462-2920.16157 · Cited by 35 · see citing works
Serratore, N. D., Baker, K. M., Macadlo, L. A., Gress, A. R., Powers, B. L., Atallah, N., Westerhouse, K. M., Hall, M. C., Weake, V. M., & Briggs, S. D. (2018) · Genetics · doi:10.1534/genetics.117.300554 · Cited by 33 · see citing works
Genome-Wide Expression Profile of Sake Brewing Yeast under Shaking and Static Conditions
Shobayashi, M., Ukena, E., Fujii, T., & Iefuji, H. (2007) · Bioscience Biotechnology and Biochemistry · doi:10.1271/bbb.60190 · Cited by 31 · see citing works
Coordination of hypoxia adaptation and iron homeostasis in human pathogenic fungi
Chung, D., Haas, H., & Cramer, R. A. (2012) · Frontiers in Microbiology · doi:10.3389/fmicb.2012.00381 · Cited by 29 · see citing works
Zhang, K., Tong, M., Gao, K., Di, Y., Wang, P., Zhang, C., Wu, X., & Zheng, D. (2014) · Journal of Industrial Microbiology & Biotechnology · doi:10.1007/s10295-014-1556-7 · Cited by 29 · see citing works
Holland, P., Bergenholm, D., Börlin, C. S., Liu, G., & Nielsen, J. (2019) · Nucleic Acids Research · doi:10.1093/nar/gkz253 · Cited by 25 · see citing works
Sinha, A., Israeli, R., Cirigliano, A., Gihaz, S., Trabelcy, B., Braus, G. H., Gerchman, Y., Fishman, A., Negri, R., Rinaldi, T., et al. (2020) · The FASEB Journal · doi:10.1096/fj.201902487r · Cited by 22 · see citing works
Stuecker, T. N., Scholes, A. N., & Lewis, J. A. (2018) · PLoS Genetics · doi:10.1371/journal.pgen.1007335 · Cited by 20 · see citing works
Ollinger, T. L., Vu, B. G., Murante, D., Parker, J. E., Šimoničová, L., Doorley, L. A., Stamnes, M. A., Kelly, S. L., Rogers, P. D., Moye-Rowley, S., et al. (2021) · mSphere · doi:10.1128/msphere.00830-21 · Cited by 14 · see citing works
Stefanini, I., Trabocchi, A., Marchi, E., Guarna, A., & Cavalieri, D. (2010) · Journal of Biological Chemistry · doi:10.1074/jbc.m110.125153 · Cited by 13 · see citing works
High expression of unsaturated fatty acid synthesis gene OLE1 in sake yeasts
Yamada, T., Shimoi, H., & Ito, K. (2005) · Journal of Bioscience and Bioengineering · doi:10.1263/jbb.99.512 · Cited by 13 · see citing works
Self-citationProgress in the genomics and genome-wide study of sake yeast
Akao, T. (2019) · Bioscience Biotechnology and Biochemistry · doi:10.1080/09168451.2019.1588098 · Cited by 12 · see citing works
Eliodório, K. P., Cunha, G. C. D. G. E., White, B. A., Patel, D. H. M., Zhang, F., Hettema, E. H., Basso, T. O., Gombert, A. K., & Raghavendran, V. (2022) · Applied and Environmental Microbiology · doi:10.1128/aem.02068-21 · Cited by 12 · see citing works
Ogihara, F., Kitagaki, H., Wang, Q., & Shimoi, H. (2008) · Yeast · doi:10.1002/yea.1596 · Cited by 11 · see citing works
Self-citationEffect of amino acids on peptide transport in sake yeast
Yamada, T., Furukawa, K., Hara, S., & Mizoguchi, H. (2005) · Journal of Bioscience and Bioengineering · doi:10.1263/jbb.99.383 · Cited by 11 · see citing works
Self-citationBiological functions of endophytic bacteria in Robinia pseudoacacia ‘Hongsen’
Huang, M., Chen, L., Ma, J., Jingzhi, M., He, L., Liang, Q., Peng, G., & Tan, Z. (2023) · Frontiers in Microbiology · doi:10.3389/fmicb.2023.1128727 · Cited by 7 · see citing works
Allele-Specific Behavior of Molecular Networks: Understanding Small-Molecule Drug Response in Yeast
Zhang, F., Gao, B., Xu, L., Li, C., Hao, D., Zhang, S., Zhou, M., Su, F., Chen, X., Zhi, H., et al. (2013) · PLoS ONE · doi:10.1371/journal.pone.0053581 · Cited by 6 · see citing works
Importance and mechanisms ofS-adenosylmethionine and folate accumulation in sake yeast
Kanai, M., Mizunuma, M., Fujii, T., & Iefuji, H. (2023) · FEMS Yeast Research · doi:10.1093/femsyr/foad004 · Cited by 5 · see citing works
Trabocchi, A., Cavalieri, D., & Guarna, A. (2011) · Pure and Applied Chemistry · doi:10.1351/pac-con-10-09-30 · Cited by 5 · see citing works
Saha, D., Gregor, J. B., Hoda, S., Eastman, K. E., Gutierrez-Schultz, V. A., Navarrete, M., Wisecaver, J. H., & Briggs, S. D. (2024) · mSphere · doi:10.1128/msphere.00524-24 · Cited by 4 · see citing works
de, L. S., Barreto, J. A., de Souza, D. T., Jordan, D. O., de Souza, S. F. D., Marin, D. F. C., da Silva, J. J., Brienzo, M., dos Santos, L. V., Rabelo, S. C., et al. (2026) · Biotechnology for Biofuels and Bioproducts · doi:10.1186/s13068-026-02748-4 · Cited by 3 · see citing works
The Genome Analysis of Sake Yeast Kyokai No. 7 and Foresights of Sake Yeast Genomics
Akao, T. (2012) · JOURNAL OF THE BREWING SOCIETY OF JAPAN · doi:10.6013/jbrewsocjapan.107.366 · Cited by 3 · see citing works
Pinel, D., Gawand, P., Mahadevan, R., & Martin, V. J. J. (2011) · Biofuels · doi:10.4155/bfs.11.120 · Cited by 2 · see citing works
Molecular basis of stress resistance in Saccharomyces cerevisiae
Heer, D. (2009) · Repository for Publications and Research Data (ETH Zurich) · doi:10.3929/ethz-a-006032739 · Cited by 1 · see citing works
Ollinger, T. L., Vu, B. G., Murante, D., Parker, J. E., Šimoničová, L., Doorley, L. A., Stamnes, M. A., Kelly, S. L., Rogers, P. D., Moye-Rowley, S., et al. (2021) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2021.10.07.463606 · Cited by 1 · see citing works
Features of sake yeast from the viewpoint of gene expression
Shobayashi, M., & Iefuji, H. (2009) · JOURNAL OF THE BREWING SOCIETY OF JAPAN · doi:10.6013/jbrewsocjapan.104.144
(2005) · Yeast · doi:10.1002/yea.1165
Fisiologia da fermentação alcoólica
de Carvalho-Netto, O. V. (2012) · doi:10.47749/t/unicamp.2012.876986
Saha, D., Gregor, J. B., Hoda, S., Eastman, K. E., Navarrete, M., Wisecaver, J. H., & Briggs, S. D. (2024) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2024.06.20.599910
- Authors
- Tamura, K.-I., Gu, Y., Wang, Q., Yamada, T., Ito, K., & Shimoi, H.
- Affiliations
- Takara Shuzo Co., Ltd. (Japan) (JP)
- Shihezi University (CN)
- Hiroshima University (JP)
- KIKU-Masamune SAKE Brewing Co., Ltd. (Japan) (JP)
- Corresponding author
- Shimoi, Hitoshi
- Venue
- Journal of Bioscience and Bioengineering
- Publisher
- Elsevier BV
- Published
- 2004-01
- Volume
- 98
- Issue
- 3
- Pages
- 159-166
- ISSN
- 1347-4421 (ISSN-L)
- 1389-1723
- OpenAlex
- W1982988838
- Cited by
- 46
- References
- 30
- Citations by year
- 2026: 1 · 2024: 3 · 2023: 2 · 2022: 2 · 2021: 2 · 2020: 2 · 2019: 2 · 2018: 1 · 2017: 1 · 2014: 3 · 2013: 1 · 2012: 3
- FWCI
- 1.18
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Bioscience and Bioengineering (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Japan International Cooperation Agency
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Yeasts and Rust Fungi Studies
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Keywords
- HAP1, ergosterol biosynthesis, gene expression regulation, transcriptional activation, sake yeast, Saccharomyces cerevisiae
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review