Characterizing Embryonic Gene Expression Patterns in the Mouse Using Nonredundant Sequence-Based Selection
Open accessAbstract
This article investigates the expression patterns of 160 genes that are expressed during early mouse development. The cDNAs were isolated from 7.5 d postcoitum (dpc) endoderm, a region that comprises visceral endoderm (VE), definitive endoderm, and the node-tissues that are required for the initial steps of axial specification and tissue patterning in the mouse. To avoid examining the same gene more than once, and to exclude potentially ubiquitously expressed housekeeping genes, cDNA sequence was derived from 1978 clones of the Endoderm library. These yielded 1440 distinct cDNAs, of which 123 proved to be novel in the mouse. In situ hybridization analysis was carried out on 160 of the cDNAs, and of these, 29 (18%) proved to have restricted expression patterns.
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Previous studies have used cDNA cloning or microarray analysis to screen for novel endoderm genes.
“Previous studies focusing on screening for novel endoderm genes have used cDNA cloning or microarray analysis [25-29,40].”
From A systematic screen for genes expressed in definitive endoderm by Serial Analysis of Gene Expression (SAGE) (2007) · cites this paper as [40]
Sousa-Nunes et al. identified 29/160 (18%) genes with restricted expression patterns from E7.5 mouse endoderm cDNA libraries using non-redundant sequence-based selection and in situ hybridization, but not all were endoderm enriched.
“Sousa-Nunes et al. identified 29/160 (18%) genes with restricted expression patterns from E7.5 mouse endoderm cDNA libraries using non-redundant sequence-based selection and in situ hybridization, but not all of these genes were endoderm enriched in their expression [40,41].”
From A systematic screen for genes expressed in definitive endoderm by Serial Analysis of Gene Expression (SAGE) (2007) · cites this paper as [40]
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Cites
The Draft Genome of <i>Ciona intestinalis</i> : Insights into Chordate and Vertebrate Origins
Dehal, P., Satou, Y., Campbell, R. K., Chapman, J., Degnan, B., De Tomaso, A., Davidson, B., Di Gregorio, A., Gelpke, M., Goodstein, D. M., et al. (2002) · Science · doi:10.1126/science.1080049 · Cited by 1,641 · cited 3× · see citing works
“…, human, mouse, and Drosophila (The C. elegans genome consortium 1998; Adams et al. 2000; Lander et al. 2001; Venter et al. 2001; Aparicio et al. 2002; Carlton et al. 2002; Dehal et al. 2002; Gardner et al. 2002; Waterston et al. 2002), and the sequences of others will be available very soon.”
Flagged first, then by citations
Research works printed but not linked: 2
- Hogan, B., Beddington, R., Costantini, F., and Lacy, E. 1994. Manipulating the mouse embryo: A laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
- Wilkinson, D.G. 1992. Whole mount in situ hybridisation of vertebrate embryos. In In situ hybridisation, pp. 75-83. IRL Press, Oxford, UK.
Cited by
Sorted by citations
Definitive endoderm of the mouse embryo: Formation, cell fates, and morphogenetic function
Lewis, S. L., & Tam, P. P. L. (2006) · Developmental Dynamics · doi:10.1002/dvdy.20846 · Cited by 164 · see citing works
Hou, J., Charters, A. M., Lee, S. C., Zhao, Y., Wu, M. K., Jones, S. J., Marra, M. A., & Hoodless, P. A. (2007) · BMC Developmental Biology · doi:10.1186/1471-213x-7-92 · Cited by 56 · see citing works
- Authors
- Sousa-Nunes, R.
- Venue
- Genome Research
- Publisher
- Cold Spring Harbor Laboratory
- Published
- 2003-12-01
- Volume
- 13
- Issue
- 12
- Pages
- 2609-2620
- ISSN
- 1088-9051 (ISSN-L)
- PMC
- PMC403803
- OpenAlex
- W2128017216
- Cited by
- 31
- References
- 1
- Citations by year
- 2025: 1 · 2024: 1 · 2021: 2 · 2018: 2 · 2015: 1 · 2012: 2
- Type
- posted content
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Genome Research (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- UCL Discovery (University College London) (submittedVersion)
- Research Portal (King's College London) (submittedVersion)
- Research at the University of Copenhagen (University of Copenhagen) (submittedVersion)
- Griffith Research Online (submittedVersion)
- UEA Digital Repository (University of East Anglia) (submittedVersion)
- Funders
- Medical Research Council
- National Health and Medical Research Council
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Phylogenetic Studies
- Keywords
- embryonic gene expression, mouse development, endoderm, visceral endoderm, definitive endoderm, tissue patterning, cDNA library, novel genes, in situ hybridization
- MeSH terms
- Animals, Crosses, Genetic, Endoderm, Mice, Inbred CBA, Mice, Inbred C57BL, Gene Library, Sequence Analysis, DNA, DNA, Complementary, Gene Expression Regulation, Developmental, Gene Expression Profiling, Mice
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
