The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
High-Throughput SNP Genotyping by Allele-Specific PCR with Universal Energy-Transfer-Labeled Primers
Open accessAbstract
We have developed a new method for high-throughput genotyping of single nucleotide polymorphisms (SNPs). The technique involves PCR amplification of genomic DNA with two tailed allele-specific primers that introduce priming sites for universal energy-transfer-labeled primers. The output of red and green light is conveniently scored using a fluorescence plate reader. The new method, which was validated on nine model SNPs, is well suited for high-throughput, automated genotyping because it requires only one reaction per SNP, it is performed in a single tube with no post-PCR handling, the same energy-transfer-labeled primers are used for all analyses, and the instrumentation is inexpensive. Possible applications include multiple-candidate gene analysis, genomewide scans, and medical diagnostics.
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Citations
Cites
Single-tube genotyping without oligonucleotide probes
Germer, S., & Higuchi, R. (1999) · Genome Research · doi:10.1101/gr.9.1.72 · Cited by 199
Cited by
Accessing genetic variation: genotyping single nucleotide polymorphisms
Syvänen, A.-C. (2001) · Nature Reviews Genetics · doi:10.1038/35103535 · Cited by 1,083
SNP Genotyping: Technologies and Biomedical Applications
Kim, S., & Misra, A. (2007) · Annual Review of Biomedical Engineering · doi:10.1146/annurev.bioeng.9.060906.152037 · Cited by 622
Methods for Genotyping Single Nucleotide Polymorphisms
Kwok, P.-Y. (2001) · Annual Review of Genomics and Human Genetics · doi:10.1146/annurev.genom.2.1.235 · Cited by 580
Sookoian, S., Castaño, G. O., Burgueño, A. L., Gianotti, T. F., Rosselli, M. S., & Pirola, C. J. (2009) · Journal of Lipid Research · doi:10.1194/jlr.p900013-jlr200 · Cited by 370
An Efficient SNP System for Mouse Genome Scanning and Elucidating Strain Relationships
Petkov, P. M., Ding, Y., Cassell, M. A., Zhang, W., Wagner, G., Sargent, E. E., Asquith, S., Crew, V., Johnson, K. A., Robinson, P., et al. (2004) · Genome Research · doi:10.1101/gr.2825804 · Cited by 340
Metadata
- Authors
- Myakishev, M. V., Khripin, Y., Hu, S., & Hamer, D. H.
- Venue
- Genome Research
- Publisher
- Cold Spring Harbor Laboratory
- Published
- 2001-01-01
- Volume
- 11
- Issue
- 1
- Pages
- 163-169
- ISSN
- 1088-9051 (ISSN-L)
- 1549-5469
- OpenAlex
- W2005330278
- Cited by
- 325
- References
- 1
- Type
- posted content
- Language
- en
- Open access
- bronze
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- Myakishev, M. V., Khripin, Y., Hu, S., & Hamer, D. H. (2001). High-Throughput SNP Genotyping by Allele-Specific PCR with Universal Energy-Transfer-Labeled Primers. Genome Research. https://doi.org/10.1101/gr.157901
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