The DOI matches its Crossref record; 5 of the 5 references it cites were found in a registry or an index.
Crystal Violet Assay for Determining Viability of Cultured Cells
Abstract
Adherent cells detach from cell culture plates during cell death. This characteristic can be used for the indirect quantification of cell death and to determine differences in proliferation upon stimulation with death-inducing agents. One simple method to detect maintained adherence of cells is the staining of attached cells with crystal violet dye, which binds to proteins and DNA. Cells that undergo cell death lose their adherence and are subsequently lost from the population of cells, reducing the amount of crystal violet staining in a culture. This protocol describes a quick and reliable screening method that is suitable for the examination of the impact of chemotherapeutics or other compounds on cell survival and growth inhibition. However, characterization of the cause of reduced crystal violet staining requires additional methods detailed elsewhere.
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Cites
Normal keratinization in a spontaneously immortalized aneuploid human keratinocyte cell line.
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Mixed Lineage Kinase Domain-like Protein Mediates Necrosis Signaling Downstream of RIP3 Kinase
Sun, L., Wang, H., Wang, Z., He, S., Chen, S., Liao, D., Wang, L., Yan, J., Liu, W., Lei, X., et al. (2012) · Cell · doi:10.1016/j.cell.2011.11.031 · Cited by 2,698
Identification of RIP1 kinase as a specific cellular target of necrostatins
Degterev, A., Hitomi, J., Germscheid, M., Ch'en, I. L., Korkina, O., Teng, X., Abbott, D., Cuny, G. D., Yuan, C., Wagner, G., et al. (2008) · Nature Chemical Biology · doi:10.1038/nchembio.83 · Cited by 2,059
Feoktistova, M., Geserick, P., Kellert, B., Dimitrova, D. P., Langlais, C., Hupe, M., Cain, K., MacFarlane, M., Häcker, G., & Leverkus, M. (2011) · Molecular Cell · doi:10.1016/j.molcel.2011.06.011 · Cited by 860
Self-citationCellular IAPs inhibit a cryptic CD95-induced cell death by limiting RIP1 kinase recruitment
Geserick, P., Hupe, M., Moulin, M., Wong, W. W.-L., Feoktistova, M., Kellert, B., Gollnick, H., Silke, J., & Leverkus, M. (2009) · Journal of Cell Biology · doi:10.1083/jcb.200904158 · Cited by 246
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Guidelines for cell viability assays
Kamiloglu, S., Sari, G., Ozdal, T., & Capanoglu, E. (2020) · Food Frontiers · doi:10.1002/fft2.44 · Cited by 769
Hamouda, R. A., Hussein, M. H., Abo-elmagd, R. A., & Bawazir, S. S. (2019) · Scientific Reports · doi:10.1038/s41598-019-49444-y · Cited by 658
Highly adhesive carbon quantum dots from biogenic amines for prevention of biofilm formation
Jian, H.-J., Yu, J., Li, Y.-J., Unnikrishnan, B., Huang, Y.-F., Luo, L.-J., Hui-Kang Ma, D., Harroun, S. G., Chang, H.-T., Lin, H.-J., et al. (2020) · Chemical Engineering Journal · doi:10.1016/j.cej.2019.123913 · Cited by 115
Linares, J., Sallent-Aragay, A., Badia-Ramentol, J., Recort-Bascuas, A., Méndez, A., Manero-Rupérez, N., Re, D. L., Rivas, E. I., Guiu, M., Zwick, M., et al. (2023) · Nature Communications · doi:10.1038/s41467-023-36334-1 · Cited by 109
Targeting breast cancer stem cells by novel HDAC3-selective inhibitors
Hsieh, H.-Y., Chuang, H.-C., Shen, F.-H., Detroja, K., Hsin, L.-W., & Chen, C.-S. (2017) · European Journal of Medicinal Chemistry · doi:10.1016/j.ejmech.2017.08.069 · Cited by 67
Metadata
- Authors
- Feoktistova, M., Geserick, P., & Leverkus, M.
- Venue
- Cold Spring Harbor Protocols
- Publisher
- Cold Spring Harbor Laboratory
- Published
- 2016-04
- Volume
- 2016
- Issue
- 4
- Pages
- pdb.prot087379
- ISSN
- 1559-6095 (ISSN-L)
- 1940-3402
- OpenAlex
- W2312266417
- Cited by
- 887
- References
- 5
- Type
- posted content
- Language
- en
- Open access
- closed
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- Feoktistova, M., Geserick, P., & Leverkus, M. (2016). Crystal Violet Assay for Determining Viability of Cultured Cells. Cold Spring Harbor Protocols. https://doi.org/10.1101/pdb.prot087379
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