Normal keratinization in a spontaneously immortalized aneuploid human keratinocyte cell line.
Open accessAbstract
In contrast to mouse epidermal cells, human skin keratinocytes are rather resistant to transformation in vitro. Immortalization has been achieved by SV40 but has resulted in cell lines with altered differentiation. We have established a spontaneously transformed human epithelial cell line from adult skin, which maintains full epidermal differentiation capacity. This HaCaT cell line is obviously immortal (greater than 140 passages), has a transformed phenotype in vitro (clonogenic on plastic and in agar) but remains nontumorigenic. Despite the altered and unlimited growth potential, HaCaT cells, similar to normal keratinocytes, reform an orderly structured and differentiated epidermal tissue when transplanted onto nude mice. Differentiation-specific keratins (Nos. 1 and 10) and other markers (involucrin and filaggrin) are expressed and regularly located. Thus, HaCaT is the first permanent epithelial cell line from adult human skin that exhibits normal differentiation and provides a promising tool for studying regulation of keratinization in human cells. On karyotyping this line is aneuploid (initially hypodiploid) with unique stable marker chromosomes indicating monoclonal origin. The identity of the HaCaT line with the tissue of origin was proven by DNA fingerprinting using hypervariable minisatellite probes. This is the first demonstration that the DNA fingerprint pattern is unaffected by long-term cultivation, transformation, and multiple chromosomal alterations, thereby offering a unique possibility for unequivocal identification of human cell lines. The characteristics of the HaCaT cell line clearly document that spontaneous transformation of human adult keratinocytes can occur in vitro and is associated with sequential chromosomal alterations, though not obligatorily linked to major defects in differentiation.
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
The HaCaT, HUVEC, SV-HUC-1, and hEM15A cell lines were totally free from cross-contamination.
“The HaCaT [34], HUVEC [35], SV-HUC-1[36], and hEM15A cell lines established from immortalized human skin cutis cells, umbilical cords, ureteral epithelium and endometrial stromal cells were totally free from cross-contamination.”
From Investigation of Cross-Contamination and Misidentification of 278 Widely Used Tumor Cell Lines (2017) · cites this paper as [34]
HaCaT cell line is immortal (>140 passages) but remains nontumorigenic, and is aneuploid (hypotetraploid) with unique stable marker chromosomes indicating monoclonal origin.
“This cell line is immortal (>140 passages) but remains nontumorigenic, and it is aneuploid (hypotetraploid) with unique stable marker chromosomes indicating monoclonal origin [40].”
From The Effect of Lycopene Preexposure on UV‐B‐Irradiated Human Keratinocytes (2016) · cites this paper as [40]
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Cites
Moll, R., Franke, W. W., Schiller, D. L., Geiger, B., & Krepler, R. (1982) · Cell · doi:10.1016/0092-8674(82)90400-7 · Cited by 5,239 · see citing works
Rheinwatd, J. G., & Green, H. (1975) · Cell · doi:10.1016/s0092-8674(75)80001-8 · Cited by 4,528 · see citing works
Hypervariable ‘minisatellite’ regions in human DNA
Jeffreys, A. J., Wilson, V., & Thein, S. L. (1985) · Nature · doi:10.1038/314067a0 · Cited by 3,771 · see citing works
Calcium regulation of growth and differentiation of mouse epidermal cells in culture
Hennings, H., Michael, D., Cheng, C., Steinert, P. M., Holbrook, K. A., & Yuspa, S. H. (1980) · Cell · doi:10.1016/0092-8674(80)90406-7 · Cited by 1,798 · see citing works
Scott, I. R., & Harding, C. R. (1986) · Developmental Biology · doi:10.1016/0012-1606(86)90230-7 · Cited by 326 · see citing works
Rheinwald, J. G., & Beckett, M. A. (1980) · Cell · doi:10.1016/0092-8674(80)90373-6 · Cited by 324 · see citing works
Neoplastic Transformation of Human Epidermal Keratinocytes by AD12-SV40 and Kirsten Sarcoma Viruses
Rhim, J. S., Jay, G., Arnstein, P., Price, F. M., Sanford, K. K., & Aaronson, S. A. (1985) · Science · doi:10.1126/science.2579430 · Cited by 216 · see citing works
Eisinger, M., Lee, J. S., Hefton, J. M., Darżynkiewicz, Z., Chiao, J.-W., & de Harven, É. (1979) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.76.10.5340 · Cited by 199 · see citing works
Transformation of Human Bronchial Epithelial Cells Transfected by Harvey ras Oncogene
Yoakum, G. H., Lechner, J. F., Gabrielson, E. W., Korba, B. E., Malan-Shibley, L. B., Willey, J. C., Valerio, M. G., Shamsuddin, A. M., Trump, B. F., & Harris, C. C. (1985) · Science · doi:10.1126/science.3975607 · Cited by 191 · see citing works
Watt, F. M. (1984) · The Journal of Cell Biology · doi:10.1083/jcb.98.1.16 · Cited by 186 · see citing works
Synthetic peptides corresponding to keratin subunits elicit highly specific antibodies.
Roop, D. R., Cheng, C., Titterington, L., Meyers, C. A., Stanley, J. R., Steinert, P. M., & Yuspa, S. H. (1984) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(17)39682-5 · Cited by 184 · see citing works
Growth and differentiation of human keratinocytes without a feeder layer or conditioned medium
Peehl, D. M., & Ham, R. G. (1980) · In Vitro Cellular & Developmental Biology - Plant · doi:10.1007/bf02626465 · Cited by 161 · see citing works
Effects of SV40 transformation on the cytoskeleton and behavioural properties of human keratinocytes
Taylor‐Papadimitriou, J., Purkis, P. E., Lane, E. B., McKay, I. A., & Chang, S. E. (1982) · Cell Differentiation · doi:10.1016/0045-6039(82)90008-2 · Cited by 160 · see citing works
Optimized Conditions for the Growth of Human Epidermal Cells in Culture
Hawley‐Nelson, P., Sullivan, J. E., Kung, M., Hennings, H., & Yuspa, S. H. (1980) · Journal of Investigative Dermatology · doi:10.1111/1523-1747.ep12522602 · Cited by 140 · see citing works
Resistance of human cells to tumorigenesis induced by cloned transforming genes.
Sager, R., Tanaka, K., Lau, C. C., Ebina, Y., & Anisowicz, A. (1983) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.80.24.7601 · Cited by 139 · see citing works
Relative Difficulties in Transforming Human and Animal Cells in Vitro
DiPaolo, J. A. (1983) · JNCI Journal of the National Cancer Institute · doi:10.1093/jnci/70.1.3 · Cited by 137 · see citing works
Bohnert, A., Hornung, J., Mackenzie, I., & Fusenig, N. E. (1986) · Cell and Tissue Research · doi:10.1007/bf00219217 · Cited by 129 · see citing works
Self-citationBowden, P. E., Quinlan, R. A., Breitkreutz, D., & Fusenig, N. E. (1984) · European Journal of Biochemistry · doi:10.1111/j.1432-1033.1984.tb08246.x · Cited by 113 · see citing works
Self-citationFusenig, N. E., & Worst, P. K. M. (1975) · Experimental Cell Research · doi:10.1016/0014-4827(75)90471-1 · Cited by 106 · see citing works
Self-citationAltered pattern of growth and differentiation in human keratinocytes infected by simian virus 40.
Steinberg, M. L., & Defendi, V. (1979) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.76.2.801 · Cited by 106 · see citing works
Differentiation of human epidermal cells transformed by SV40.
Banks-Schlegel, S. P., & Howley, P. M. (1983) · The Journal of Cell Biology · doi:10.1083/jcb.96.2.330 · Cited by 104 · see citing works
Leigh, I. M., Pulford, K. A. F., Ramaekers, F. C. S., & Lane, E. B. (1985) · British Journal of Dermatology · doi:10.1111/j.1365-2133.1985.tb02044.x · Cited by 94 · see citing works
Boukamp, P., Tilgen, W., Dzarlieva, R., Breitkreutz, D., Haag, D., Riehl, R. K., Bohnert, A., & Fusenig, N. E. (1982) · JNCI Journal of the National Cancer Institute · doi:10.1093/jnci/68.3.415 · Cited by 90 · see citing works
Self-citationDifferentiation specific functions in cultured and transplanted mouse keratinocytes:
Breitkreutz, D., Bohnert, A., Herzmann, E., Bowden, P. E., Boukamp, P., & Fusenig, N. E. (1984) · Differentiation · doi:10.1111/j.1432-0436.1984.tb01389.x · Cited by 86 · see citing works
Self-citationWorst, P. K. M., Mackenzie, I. C., & Fusenig, N. E. (1982) · Cell and Tissue Research · doi:10.1007/bf00216219 · Cited by 80 · see citing works
Self-citationTransformation and Immortalization of Human Keratinocytes by SV40
Steinberg, M. L., & Defendi, V. (1983) · Journal of Investigative Dermatology · doi:10.1111/1523-1747.ep12540905 · Cited by 77 · see citing works
Weissman, B. E., & Aaronson, S. A. (1985) · Molecular and Cellular Biology · doi:10.1128/mcb.5.12.3386 · Cited by 75 · see citing works
Boukamp, P., Rupniak, H. T., & Fusenig, N. E. (1985) · PubMed · Cited by 74 · see citing works
Self-citationTilgen, W., Boukamp, P., Breitkreutz, D., Dzarlieva, R., Engstner, M., Haag, D., & Fusenig, N. E. (1983) · PubMed · Cited by 71 · see citing works
Self-citationBaden, H. P., Kubilus, J., Kvedar, J. C., Steinberg, M. L., & Wolman, S. R. (1987) · In Vitro Cellular & Developmental Biology - Plant · doi:10.1007/bf02623581 · Cited by 65 · see citing works
Yuspa, S. H., Vass, W., & Scolnick, E. M. (1983) · PubMed · Cited by 61 · see citing works
Cytogenetically marked clones in human fibroblasts cultured from normal subjects
Harnden, D. G., BENN, P. A., Oxford, J. M., Taylor, A. M. R., & Webb, T. (1976) · Somatic Cell and Molecular Genetics · doi:10.1007/bf01539242 · Cited by 58 · see citing works
Effect of growth environment on spatial expression of involucrin by human epidermal keratinocytes
Watt, F. M., Boukamp, P., Hornung, J., & Fusenig, N. E. (1987) · Archives of Dermatological Research · doi:10.1007/bf00431227 · Cited by 53 · see citing works
Self-citationReexpression of fetal characters in simian virus 40-transformed human keratinocytes.
Bernard, B., Robinson, S. M., Sémat, A., & Darmon, M. Y. (1985) · PubMed · Cited by 49 · see citing works
Sanford, K. K., & Evans, V. J. (1982) · JNCI Journal of the National Cancer Institute · doi:10.1093/jnci/68.6.895 · Cited by 48 · see citing works
Spontaneous transformation of human skin fibroblasts derived from neoplastic patients
Azzarone, B. G., Pedullá, D., & ROMANZI, C. A. (1976) · Nature · doi:10.1038/262074a0 · Cited by 47 · see citing works
Mammalian Epidermal Cells in Culture
Fusenig, N. E. (1986) · Springer eBooks · doi:10.1007/978-3-662-00989-5_22 · Cited by 43 · see citing works
Self-citationLittlefield, L. G., & Mailhes, J. B. (1975) · PubMed · Cited by 42 · see citing works
Fusenig, N. E., Dzarlieva-Petruševska, R. T., & Breitkreutz, D. (1985) · PubMed · Cited by 41 · see citing works
Self-citationEnvironmental induction of differentiation-specific keratins in malignant mouse keratinocyte lines.
Breitkreutz, D., Hornung, J., Pöhlmann, J., Brown-Bierman, L., Bohnert, A., Bowden, P. E., & Fusenig, N. E. (1986) · PubMed · Cited by 37 · see citing works
Self-citationRapid Modulation of Keratinocyte Differentiation by the External Environment
Lavker, R. M., & Sun, T. (1983) · Journal of Investigative Dermatology · doi:10.1111/1523-1747.ep12534527 · Cited by 35 · see citing works
Kano, Y., & Little, J. B. (1985) · PubMed · Cited by 34 · see citing works
Keratin Expression during Normal Epidermal Differentiation
Sun, T., Eichner, R., Nelson, W. G., Vidrich, A., & Woodcock‐Mitchell, J. (2015) · Current problems in dermatology · doi:10.1159/000408682 · Cited by 34 · see citing works
Epithelial Line From Normal Human Colon Mucosa 2 3 4
Danes, B. S., & Sutanto, E. (1982) · JNCI Journal of the National Cancer Institute · doi:10.1093/jnci/69.6.1271 · Cited by 30 · see citing works
Extracellular matrix components produced by SV40-transformed human epidermal keratinocytes
Brown, K. W., & Parkinson, E. K. (1984) · International Journal of Cancer · doi:10.1002/ijc.2910330215 · Cited by 29 · see citing works
Spontaneous In Vitro Transformation of Human Fibroblasts 2
Mukherji, B., MacAlister, T. J., Guha, A., Gillies, C. G., Jeffers, D., & Slocum, S. K. (1984) · JNCI Journal of the National Cancer Institute · doi:10.1093/jnci/73.3.583 · Cited by 28 · see citing works
Baden, H. P., Kubilus, J., Wolman, S. R., Steinberg, M. L., Phillips, S. B., & Kvedar, J. C. (1987) · Journal of Investigative Dermatology · doi:10.1111/1523-1747.ep12461235 · Cited by 25 · see citing works
Spontaneous in vitro malignant transformation in a xeroderma pigmentosum fibroblast line
Thielmann, H. W., Fischer, E., Dzarlieva, R., Komittowski, D., Popanda, O., & Edler, L. (1983) · International Journal of Cancer · doi:10.1002/ijc.2910310603 · Cited by 18 · see citing works
Role of elevated temperature in malignant transformation of mammalian cells in vztro
Marczynska, B., Bergholz, C. M., & Wolfe, L. G. (1980) · International Journal of Cancer · doi:10.1002/ijc.2910250618 · Cited by 15 · see citing works
Weissman, B. E., & Aaronson, S. A. (1985) · Molecular and Cellular Biology · doi:10.1128/mcb.5.12.3386-3396.1985 · Cited by 12 · see citing works
Oncogenes and Malignant Transformation of Human Keratinocytes
Fusenig, N. E., Boukamp, P., Breitkreutz, D., Karjetta, S., & Petrusevska, R. T. (1987) · Springer eBooks · doi:10.1007/978-1-4615-6462-1_35 · Cited by 11 · see citing works
Self-citationNagasawa, H., Zamansky, G. B., McCone, E. F., Arundel, C. M., Matkin, E. D., & Little, J. B. (1987) · Journal of Investigative Dermatology · doi:10.1111/1523-1747.ep12525294 · Cited by 8 · see citing works
Mammalian cell transformation. Mechanisms of carcinogenesis and assays for carcinogens.
Barrett, J. C., & Tennant, R. W. (1985) · PubMed · Cited by 7 · see citing works
Flagged first, then by citations
Cited by
Sorted by citations
pl5INK4B is a potentia| effector of TGF-β-induced cell cycle arrest
Hannon, G. J., & Beach, D. (1994) · Nature · doi:10.1038/371257a0 · Cited by 1,950 · see citing works
Spontaneous Human Adult Stem Cell Transformation
Rubio, D., Garcı́a-Castro, J., Martín, M. C., de la Fuente, R., Cigudosa, J. C., Lloyd, A. C., & Bernad, A. (2005) · Cancer Research · doi:10.1158/0008-5472.can-04-4194 · Cited by 1,030 · see citing works
RetractedG-quadruplex structures mark human regulatory chromatin
Hänsel-Hertsch, R., Beraldi, D., Lensing, S. V., Marsico, G., Zyner, K., Parry, A., Di Antonio, M., Pike, J., Kimura, H., Narita, M., et al. (2016) · Nature Genetics · doi:10.1038/ng.3662 · Cited by 964 · see citing works
Kip/Cip and Ink4 Cdk inhibitors cooperate to induce cell cycle arrest in response to TGF-beta.
Reynisdóttir, I., Polyak, K., Iavarone, A., & Massagué, J. (1995) · Genes & Development · doi:10.1101/gad.9.15.1831 · Cited by 962 · see citing works
Datto, M., Li, Y., Panus, J. F., Howe, D. J., Xiong, Y., & Wang, X. F. (1995) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.92.12.5545 · Cited by 945 · see citing works
Crystal Violet Assay for Determining Viability of Cultured CellsPreprint
Feoktistova, M., Geserick, P., & Leverkus, M. (2016) · Cold Spring Harbor Protocols · doi:10.1101/pdb.prot087379 · Cited by 894 · see citing works
Photocrosslinkable Gelatin Hydrogel for Epidermal Tissue Engineering
Zhao, X., Lang, Q., Yildirimer, L., Lin, Z. Y., Cui, W., Annabi, N., Ng, K. W., Dokmeci, M. R., Ghaemmaghami, A. M., & Khademhosseini, A. (2015) · Advanced Healthcare Materials · doi:10.1002/adhm.201500005 · Cited by 839 · see citing works
Regulation of Vascular Endothelial Growth Factor Expression in Cultured Keratinocytes.
Frank, S., Hübner, G., Breier, G., Longaker, M. T., Greenhalgh, D. G., & Werner, S. (1995) · Journal of Biological Chemistry · doi:10.1074/jbc.270.21.12607 · Cited by 764 · see citing works
Wang, X., Tang, S., Le, S., Lu, R., Rader, J. S., Meyers, C., & Zheng, Z. (2008) · PLoS ONE · doi:10.1371/journal.pone.0002557 · Cited by 736 · see citing works
Guan, K., Jenkins, C. W., Li, Y., Nichols, M. A., Wu, X., O'Keefe, C., Matera, A. G., & Xiong, Y. (1994) · Genes & Development · doi:10.1101/gad.8.24.2939 · Cited by 707 · see citing works
Tissue factor- and factor X-dependent activation of protease-activated receptor 2 by factor VIIa
Camerer, E., Huang, W., & Coughlin, S. R. (2000) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.97.10.5255 · Cited by 674 · see citing works
Maretzky, T., Reiß, K., Ludwig, A., Buchholz, J., Scholz, F., Proksch, E., Strooper, B. D., Hartmann, D., & Säftig, P. (2005) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0500918102 · Cited by 663 · see citing works
Dunphy, G., Flannery, S. M., Almine, J. F., Connolly, D. J., Paulus, C., Jønsson, K. L., Jakobsen, M. R., Nevels, M. M., Bowie, A. G., & Unterholzner, L. (2018) · Molecular Cell · doi:10.1016/j.molcel.2018.07.034 · Cited by 648 · see citing works
“HaCaT cells are spontaneously immortalized and express mutated forms of p53, which are partially functional, but may also have acquired additional functions (Boukamp et al., 1988; Lehman et al., 1993).”
BackgroundSignal characteristics of G protein‐transactivated EGF receptor
Daub, H., Wallasch, C., Lankenau, A., Herrlich, A., & Ullrich, A. (1997) · The EMBO Journal · doi:10.1093/emboj/16.23.7032 · Cited by 647 · see citing works
The Activity of Collagenase-1 Is Required for Keratinocyte Migration on a Type I Collagen Matrix
Pilcher, B. K., Dumin, J. A., Sudbeck, B. D., Krane, S. M., Welgus, H. G., & Parks, W. C. (1997) · The Journal of Cell Biology · doi:10.1083/jcb.137.6.1445 · Cited by 613 · see citing works
Miranda, T. B., Cortez, C. C., Yoo, C. B., Liang, G., Abe, M., Kelly, T. K., Márquez, V. E., & Jones, P. A. (2009) · Molecular Cancer Therapeutics · doi:10.1158/1535-7163.mct-09-0013 · Cited by 588 · see citing works
A Self-Enabling TGFβ Response Coupled to Stress Signaling
Kang, Y., Chen, C.-R., & Massagué, J. (2003) · Molecular Cell · doi:10.1016/s1097-2765(03)00109-6 · Cited by 548 · see citing works
Blumberg, H., Conklin, D., Xu, W., Grossmann, A., Brender, T., Carollo, S., Eagan, M., Foster, D., Haldeman, B. A., Hammond, A., et al. (2001) · Cell · doi:10.1016/s0092-8674(01)00187-8 · Cited by 542 · see citing works
Genetic programs of epithelial cell plasticity directed by transforming growth factor-β
Zavadil, J., Bitzer, M., Liang, D., Yang, Y.-C., Massimi, A. B., Kneitz, S., Piek, E., & Böttinger, E. P. (2001) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.111614398 · Cited by 534 · see citing works
Copper-induced vascular endothelial growth factor expression and wound healing
Sen, C. K., Khanna, S., Venojarvi, M., Trikha, P., Ellison, E. C., Hunt, T. K., & Roy, S. (2002) · American Journal of Physiology-Heart and Circulatory Physiology · doi:10.1152/ajpheart.01015.2001 · Cited by 522 · see citing works
López‐García, J., Lehocký, M., Humpolíček, P., & Sáha, P. (2014) · Journal of Functional Biomaterials · doi:10.3390/jfb5020043 · Cited by 518 · see citing works
Walter, M. N. M., Wright, K. T., Fuller, H. R., MacNeil, S., & Johnson, W. E. B. (2010) · Experimental Cell Research · doi:10.1016/j.yexcr.2010.02.026 · Cited by 496 · see citing works
Härle-Bachor, C., & Boukamp, P. (1996) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.93.13.6476 · Cited by 492 · see citing works
Self-citationEfficient gene transfer into human hepatocytes by baculovirus vectors.
Hofmann, C., Sandig, V., Jennings, G. S., Rudolph, M., Schlag, P., & Strauss, M. (1995) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.92.22.10099 · Cited by 486 · see citing works
E2F4/5 and p107 as Smad Cofactors Linking the TGFβ Receptor to c-myc Repression
Chen, C.-R., Kang, Y., Siegel, P. M., & Massagué, J. (2002) · Cell · doi:10.1016/s0092-8674(02)00801-2 · Cited by 482 · see citing works
Vasvani, S., Kulkarni, P., & Rawtani, D. (2019) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2019.11.066 · Cited by 477 · see citing works
SiO2 nanoparticles induce cytotoxicity and protein expression alteration in HaCaT cells
Yang, X., Liu, J., He, H., Zhou, L., Gong, C., Wang, X., Yang, L., Yuan, J., Huang, H., He, L., et al. (2010) · Particle and Fibre Toxicology · doi:10.1186/1743-8977-7-1 · Cited by 472 · see citing works
Surface charge of gold nanoparticles mediates mechanism of toxicity
Schaeublin, N. M., Braydich‐Stolle, L. K., Schrand, A. M., Miller, J. M., Hutchison, J., Schlager, J. J., & Hussain, S. M. (2011) · Nanoscale · doi:10.1039/c0nr00478b · Cited by 469 · see citing works
Howling, G. I., Dettmar, P. W., Goddard, P., Hampson, F. C., Dornish, M., & Wood, E. J. (2001) · Biomaterials · doi:10.1016/s0142-9612(01)00042-4 · Cited by 468 · see citing works
Aragane, Y., Kulms, D., Metze, D., Wilkes, G., Pöppelmann, B., Luger, T. A., & Schwarz, T. (1998) · The Journal of Cell Biology · doi:10.1083/jcb.140.1.171 · Cited by 463 · see citing works
Mutations in two adjacent novel genes are associated with epidermodysplasia verruciformis
Ramoz, N., Rueda, L.-A., Bouadjar, B., Montoya, L.-S., Orth, G., & Fãvre, M. (2002) · Nature Genetics · doi:10.1038/ng1044 · Cited by 450 · see citing works
Karikó, K., Bhuyan, P. K., Capodici, J., & Weissman, D. (2004) · The Journal of Immunology · doi:10.4049/jimmunol.172.11.6545 · Cited by 446 · see citing works
Fritsche, E., Schäfer, C., Calles, C., Bernsmann, T., Bernshausen, T., Wurm, M., Hübenthal, U., Cline, J. E., Hajimiragha, H., Schroeder, P., et al. (2007) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0701764104 · Cited by 437 · see citing works
Non-Thermal Atmospheric-Pressure Plasma Possible Application in Wound Healing
Haertel, B., von Woedtke, T., Weltmann, K., & Lindequist, U. (2014) · Biomolecules & Therapeutics · doi:10.4062/biomolther.2014.105 · Cited by 437 · see citing works
Datto, M., Yu, Y., & Wang, X. (1995) · Journal of Biological Chemistry · doi:10.1074/jbc.270.48.28623 · Cited by 429 · see citing works
Halting angiogenesis suppresses carcinoma cell invasion
Skobe, M., Rockwell, P., Goldstein, N. I., Vosseler, S., & Fusenig, N. E. (1997) · Nature Medicine · doi:10.1038/nm1197-1222 · Cited by 420 · see citing works
Self-citationAtmospheric Pressure Plasma Jet for Medical Therapy: Plasma Parameters and Risk Estimation
Weltmann, K. D., Kindel, E., Brandenburg, R., Meyer, C., Bussiahn, R., Wilke, C., & von Woedtke, T. (2009) · Contributions to Plasma Physics · doi:10.1002/ctpp.200910067 · Cited by 418 · see citing works
Braun, S., Hanselmann, C., Gassmann, M. G., auf dem Keller, U., Born-Berclaz, C., Chan, K., Kan, Y. W., & Werner, S. (2002) · Molecular and Cellular Biology · doi:10.1128/mcb.22.15.5492-5505.2002 · Cited by 410 · see citing works
Conejo-Garcia, J. R., Jaumann, F., Schulz, S., Krause, A., Rodríguez-Jiménez, J., Forssmann, U., Adermann, K., Klüver, E., Vogelmeier, C. F., Becker, D., et al. (2001) · Cell and Tissue Research · doi:10.1007/s004410100433 · Cited by 408 · see citing works
Probst, C., Schlumberger, W., STÖCKER, W., Recke, A., Schmidt, E., Hashimoto, T., Zhu, X. J., Zillikens, D., & Komorowski, L. (2009) · Clinica Chimica Acta · doi:10.1016/j.cca.2009.08.022 · Cited by 405 · see citing works
Curcumin Is an In Vivo Inhibitor of Angiogenesis
Arbiser, J. L., Klauber, N., Rohan, R. M., van Leeuwen, R., Huang, M., Fisher, C., Flynn, E. A., & Byers, H. R. (1998) · Molecular Medicine · doi:10.1007/bf03401744 · Cited by 401 · see citing works
Heme oxygenase-1 induction by NRF2 requires inactivation of the transcriptional repressor BACH1
Reichard, J. F., Motz, G. T., & Puga, A. (2007) · Nucleic Acids Research · doi:10.1093/nar/gkm638 · Cited by 393 · see citing works
Defining the molecular mechanisms of human cell immortalization
Shay, J. W., Wright, W. E., & Werbin, H. (1991) · Biochimica et Biophysica Acta (BBA) - Reviews on Cancer · doi:10.1016/0304-419x(91)90003-4 · Cited by 382 · see citing works
lavarone, A., & Massagué, J. (1997) · Nature · doi:10.1038/387417a0 · Cited by 375 · see citing works
Pastrana, D. V., Buck, C. B., Pang, Y.-Y. S., Thompson, C. D., Castle, P. E., FitzGerald, P. C., Kjær, S. K., Lowy, D. R., & Schiller, J. T. (2004) · Virology · doi:10.1016/j.virol.2003.12.027 · Cited by 358 · see citing works
Moulton, M. C., Braydich‐Stolle, L. K., Nadagouda, M. N., Kunzelman, S., Hussain, S. M., & Varma, R. S. (2010) · Nanoscale · doi:10.1039/c0nr00046a · Cited by 357 · see citing works
Colombo, I., Sangiovanni, E., Maggio, R., Mattozzi, C., Zava, S., Corbett, Y., Fumagalli, M., Carlino, C., Corsetto, P. A., Scaccabarozzi, D., et al. (2017) · Mediators of Inflammation · doi:10.1155/2017/7435621 · Cited by 356 · see citing works
Köllisch, G. V., Kalali, B. N., Voelcker, V., Wallich, R., Behrendt, H., Ring, J., Bauer, S., Jakob, T., Mempel, M., & Ollert, M. W. (2005) · Immunology · doi:10.1111/j.1365-2567.2005.02122.x · Cited by 355 · see citing works
A Transgenic Mouse Model of Inducible Macrophage Depletion
Goren, I., Allmann, N., Yogev, N., Schürmann, C., Linke, A., Holdener, M., Waisman, A., Pfeilschifter, J. M., & Frank, S. (2009) · American Journal Of Pathology · doi:10.2353/ajpath.2009.081002 · Cited by 355 · see citing works
Establishment of human papillomavirus infection requires cell cycle progression
Pyeon, D., Pearce, S. M., Lank, S. M., Ahlquist, P., & Lambert, P. F. (2009) · PLoS Pathogens · doi:10.1371/journal.ppat.1000318 · Cited by 348 · see citing works
Pece, S., & Gutkind, J. S. (2000) · Journal of Biological Chemistry · doi:10.1074/jbc.m006578200 · Cited by 345 · see citing works
Nadagouda, M. N., Castle, A. B., Murdock, R. C., Hussain, S. M., & Varma, R. S. (2009) · Green Chemistry · doi:10.1039/b921203p · Cited by 341 · see citing works
Non-melanoma skin cancer: what drives tumor development and progression?
Boukamp, P. (2005) · Carcinogenesis · doi:10.1093/carcin/bgi123 · Cited by 341 · see citing works
Self-citationPerformance of a novel keratinocyte-based reporter cell line to screen skin sensitizers in vitro
Emter, R., Ellis, G., & Natsch, A. (2010) · Toxicology and Applied Pharmacology · doi:10.1016/j.taap.2010.03.009 · Cited by 337 · see citing works
Cytotoxic Action of Juglone and Plumbagin: A Mechanistic Study Using HaCaT Keratinocytes
Inbaraj, J. J., & Chignell, C. F. (2003) · Chemical Research in Toxicology · doi:10.1021/tx034132s · Cited by 335 · see citing works
Schoop, V. M., Fusenig, N. E., & Mirancea, N. (1999) · Journal of Investigative Dermatology · doi:10.1046/j.1523-1747.1999.00524.x · Cited by 331 · see citing works
Self-citationDoorbar, J., Ely, S. J., Sterling, J. C., McLean, C. S., & Crawford, L. (1991) · Nature · doi:10.1038/352824a0 · Cited by 329 · see citing works
Sen, C. K., Khanna, S., BABIOR, B. M., Hunt, T. K., Ellison, E. C., & Roy, S. (2002) · Journal of Biological Chemistry · doi:10.1074/jbc.m203391200 · Cited by 325 · see citing works
Uberoi, A., Bartow‐McKenney, C., Zheng, Q., Flowers, L. J., Campbell, A. E., Knight, S. A. B., Chan, N., Wei, M. H., Lovins, V. M., Bugayev, J., et al. (2021) · Cell Host & Microbe · doi:10.1016/j.chom.2021.05.011 · Cited by 325 · see citing works
Molecular mechanisms of UV-induced apoptosis
Kulms, D., & Schwarz, T. (2000) · Photodermatology, Photoimmunology & Photomedicine · doi:10.1034/j.1600-0781.2000.160501.x · Cited by 319 · see citing works
Modified Vaccinia Virus Ankara
Volz, A., & Sutter, G. (2016) · Advances in virus research · doi:10.1016/bs.aivir.2016.07.001 · Cited by 318 · see citing works
Immature truncated O-glycophenotype of cancer directly induces oncogenic features
Radhakrishnan, P., Dabelsteen, S., Madsen, F. B., Francavilla, C., Kopp, K., Steentoft, C., Vakhrushev, S. Y., Olsen, J. V., Hansen, L. H., Bennett, E., et al. (2014) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1406619111 · Cited by 316 · see citing works
Selective killing of transformed cells by cyclin/cyclin-dependent kinase 2 antagonists
Chen, Y.-N. P., Sharma, S. K., Ramsey, T. M., Jiang, L., Martin, M. S., Baker, K., Adams, P. D., Bair, K. W., & Kaelin, W. G. (1999) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.96.8.4325 · Cited by 315 · see citing works
Human keratinocytes are efficiently immortalized by a Rho kinase inhibitor
Chapman, S., Liu, X., Meyers, C., Schlegel, R., & McBride, A. A. (2010) · Journal of Clinical Investigation · doi:10.1172/jci42297 · Cited by 314 · see citing works
EGF-R signaling through Fyn kinase disrupts the function of integrin α6β4 at hemidesmosomes
Mariotti, A., Kedeshian, P. A., Dans, M. J., Curatola, A. M., Gagnoux-Palacios, L., & Giancotti, F. G. (2001) · The Journal of Cell Biology · doi:10.1083/jcb.200105017 · Cited by 304 · see citing works
Biocompatibility of polyaniline
Humpolíček, P., Kašpárková, V., Sáha, P., & Stejskal, J. (2012) · Synthetic Metals · doi:10.1016/j.synthmet.2012.02.024 · Cited by 303 · see citing works
Frank, S., Stallmeyer, B., Kämpfer, H., Kolb, N., & Pfeilschifter, J. M. (2000) · Journal of Clinical Investigation · doi:10.1172/jci9148 · Cited by 301 · see citing works
Cytolysin-Mediated Translocation (CMT)
Madden, J. C., Ruiz, N., & Caparon, M. G. (2001) · Cell · doi:10.1016/s0092-8674(01)00198-2 · Cited by 300 · see citing works
Transcriptional Activation of the Human p21 Gene by Retinoic Acid Receptor
Liu, M., Iavarone, A., & Freedman, L. P. (1996) · Journal of Biological Chemistry · doi:10.1074/jbc.271.49.31723 · Cited by 300 · see citing works
Long term low-dose arsenic exposure induces loss of DNA methylation
Reichard, J. F., Schnekenburger, M., & Puga, A. (2006) · Biochemical and Biophysical Research Communications · doi:10.1016/j.bbrc.2006.11.001 · Cited by 299 · see citing works
IL-31 regulates differentiation and filaggrin expression in human organotypic skin models
Cornelissen, C., Marquardt, Y., Czaja, K., Wenzel, J., Frank, J., Lüscher‐Firzlaff, J., Lüscher, B., & Baron, J. M. (2011) · Journal of Allergy and Clinical Immunology · doi:10.1016/j.jaci.2011.10.042 · Cited by 292 · see citing works
Brandner, J. M., Kief, S., Grund, C., Rendl, M., Houdek, P., Kuhn, C., Tschachler, E., Franke, W. W., & Moll, I. (2002) · European Journal of Cell Biology · doi:10.1078/0171-9335-00244 · Cited by 289 · see citing works
Leverkus, M., Neumann, M., Mengling, T., Rauch, C. T., Bröcker, E. B., Krammer, P. H., & Walczak, H. (2000) · PubMed · Cited by 288 · see citing works
Rancan, F., Gao, Q., Graf, C. M., Troppens, S., Hadam, S., Hackbarth, S., Kembuan, C., Blume‐Peytavi, U., Rühl, E., Lademann, J. M., et al. (2012) · ACS Nano · doi:10.1021/nn301622h · Cited by 287 · see citing works
Okada, N., Liszewski, M. K., Atkinson, J. P., & Caparon, M. G. (1995) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.92.7.2489 · Cited by 285 · see citing works
Fusenig, N. E., & Boukamp, P. (1998) · Molecular Carcinogenesis · doi:10.1002/(sici)1098-2744(199811)23:3 · Cited by 282 · see citing works
Self-citationWende, K., Williams, P., Dalluge, J., Gaens, W. V., Aboubakr, H. A., Bischof, J. C., von Woedtke, T., Goyal, S. M., Weltmann, K., Bogaerts, A., et al. (2015) · Biointerphases · doi:10.1116/1.4919710 · Cited by 280 · see citing works
Scholle, F., Bendt, K. M., & Raab‐Traub, N. (2000) · Journal of Virology · doi:10.1128/jvi.74.22.10681-10689.2000 · Cited by 276 · see citing works
Geng, Y., & Weinberg, R. A. (1993) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.90.21.10315 · Cited by 273 · see citing works
Melanoma cells influence the differentiation pattern of human epidermal keratinocytes
Kodet, O., Lacina, L., Krejčí, E., Dvořánková, B., Grim, M., Štork, J., Kodetova, D. N., Vlček, Č., Šáchová, J., Kolář, M., et al. (2015) · Molecular Cancer · doi:10.1186/1476-4598-14-1 · Cited by 270 · see citing works
Lohi, J., Wilson, C. L., Roby, J. D., & Parks, W. C. (2001) · Journal of Biological Chemistry · doi:10.1074/jbc.m001599200 · Cited by 270 · see citing works
Negative growth control of HeLa cells by connexin genes: connexin species specificity.
Mesnil, M., Krutovskikh, V. A., Piccoli, C., Elfgang, C., Traub, O., Willecke, K., & Yamasaki, H. (1995) · PubMed · Cited by 269 · see citing works
Schwarz, A., Bhardwaj, R. S., Aragane, Y., Mahnke, K., Riemann, H., Metze, D., Luger, T. A., & Schwarz, T. (1995) · Journal of Investigative Dermatology · doi:10.1111/1523-1747.ep12606202 · Cited by 268 · see citing works
Capon, F., Allen, M. H., Ameen, M., Burden, A. D., Tillman, D. M., Barker, J. N., & Trembath, R. C. (2004) · Human Molecular Genetics · doi:10.1093/hmg/ddh273 · Cited by 265 · see citing works
Meißner, M., Reichert, T. E., Kunkel, M., Gooding, W. E., Whiteside, T. L., Ferrone, S., & Seliger, B. (2005) · Clinical Cancer Research · doi:10.1158/1078-0432.ccr-04-2146 · Cited by 263 · see citing works
A new approach to the toxicity testing of carbon-based nanomaterials—The clonogenic assay
Herzog, E., Casey, A., Lyng, F. M., Chambers, G., Byrne, H. J., & Davoren, M. (2007) · Toxicology Letters · doi:10.1016/j.toxlet.2007.08.009 · Cited by 263 · see citing works
Grassberger, Baumruker, Enz, Hiestand, Hultsch, Kalthoff, Schuler, �., Schulz, Werner, R., Winiski, et al. (1999) · British Journal of Dermatology · doi:10.1046/j.1365-2133.1999.02974.x · Cited by 263 · see citing works
Growth of human cells on a non-woven silk fibroin net: a potential for use in tissue engineering
Unger, R. E., Wolf, M., Peters, K., Motta, A., Migliaresi, C., & Kirkpatrick, C. J. (2003) · Biomaterials · doi:10.1016/s0142-9612(03)00619-7 · Cited by 259 · see citing works
Tumor-Selective Induction of Apoptosis and the Small-Molecule Immune Response Modifier Imiquimod
Schön, M., Bong, A. B., Drewniok, C., Herz, J., Geilen, C. C., REIFENBERGER, J., Benninghoff, B., Slade, H. B., Gollnick, H. P. M., & Schön, M. P. (2003) · JNCI Journal of the National Cancer Institute · doi:10.1093/jnci/djg016 · Cited by 258 · see citing works
Reduced expression of PDGF and PDGF receptors during impaired wound healing
Beer, H.-D., Longaker, M. T., & Werner, S. (1997) · Journal of Investigative Dermatology · doi:10.1111/1523-1747.ep12319188 · Cited by 257 · see citing works
Chmielewski, M. M., Hombach, A. A., Heuser, C., Adams, G. P., & Abken, H. (2004) · The Journal of Immunology · doi:10.4049/jimmunol.173.12.7647 · Cited by 257 · see citing works
Neumann-Giesen, C., FALKENBACH, B., BEICHT, P., CLAASEN, S., Lüers, G. H., Stuermer, C. A. O., Herzog, V., & Tikkanen, R. (2004) · Biochemical Journal · doi:10.1042/bj20031100 · Cited by 256 · see citing works
A zinc-sensing receptor triggers the release of intracellular Ca 2+ and regulates ion transport
Hershfinkel, M., Moran, A., Grossman, N., & Sekler, I. (2001) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.201193398 · Cited by 253 · see citing works
Yin, J., Liu, J., Ehrenshaft, M., Roberts, J. E., Fu, P. P., Mason, R. P., & Zhao, B. (2012) · Toxicology and Applied Pharmacology · doi:10.1016/j.taap.2012.06.001 · Cited by 251 · see citing works
Vitamin D Induces the Antimicrobial Protein hCAP18 in Human Skin
Weber, G., Heilborn, J. D., Chamorro, C. I., Hammarsjö, A., Törmä, H., & Ståhle, M. (2005) · Journal of Investigative Dermatology · doi:10.1111/j.0022-202x.2005.23687.x · Cited by 251 · see citing works
Gille, J., Swerlick, R. A., & Caughman, S. W. (1997) · The EMBO Journal · doi:10.1093/emboj/16.4.750 · Cited by 250 · see citing works
Nicola, A. V., Hou, J., Major, E. O., & Straus, S. E. (2005) · Journal of Virology · doi:10.1128/jvi.79.12.7609-7616.2005 · Cited by 247 · see citing works
Regl, G., Kasper, M., Schnidar, H., Eichberger, T., Neill, G. W., Philpott, M. P., Esterbauer, H., Hauser‐Kronberger, C., Frischauf, A., & Aberger, F. (2004) · Cancer Research · doi:10.1158/0008-5472.can-04-1085 · Cited by 247 · see citing works
Lippens, S., Kockx, M., Knaapen, M., Mortier, L., Polakowska, R., Verheyen, A., Garmyn, M., Zwijsen, A., Formstecher, P., Huylebroeck, D., et al. (2000) · Cell Death & Differentiation · doi:10.1038/sj.cdd.4400785 · Cited by 245 · see citing works
- Authors
- Boukamp, P., Petrussevska, R. T., Breitkreutz, D., Hornung, J., Markham, A., & Fusenig, N. E.
- Affiliations
- German Cancer Research Center (DE)
- Venue
- The Journal of cell biology
- Publisher
- Rockefeller University Press
- Published
- 1988-03-01
- Volume
- 106
- Issue
- 3
- Pages
- 761-771
- ISSN
- 0021-9525 (ISSN-L)
- 1540-8140
- PMC
- PMC2115116
- OpenAlex
- W2143464311
- Cited by
- 4,170
- References
- 53
- Citations by year
- 2026: 103 · 2025: 109 · 2024: 126 · 2023: 116 · 2022: 132 · 2021: 141 · 2020: 138 · 2019: 111 · 2018: 121 · 2017: 136 · 2016: 147 · 2015: 148 · 2014: 157 · 2013: 165 · 2012: 152
- FWCI
- 9.52
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Licence
- cc-by-nc-sa
- Locations
- The Journal of Cell Biology (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion, cc-by-nc-sa)
- PubMed Central (publishedVersion, cc-by-nc-sa)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Skin and Cellular Biology Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Genetic and rare skin diseases.
- Health Sciences › Medicine › Urology › Hair Growth and Disorders
- Keywords
- human keratinocytes, spontaneous immortalization, aneuploidy, epidermal differentiation, keratinization, keratin 1, keratin 10, involucrin, filaggrin, cell line identification, DNA fingerprinting, minisatellite markers, chromosomal abnormalities, karyology
- MeSH terms
- Epidermal Cells, Filaggrin Proteins, Aneuploidy, Calcium, Cell Division, Cell Line, Transformed, Chromosomes, Human, DNA, Fluorescent Antibody Technique, Humans, Karyotyping, Keratins, Male, Middle Aged, Temperature
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review