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The OP9-DL1 System: Generation of T-Lymphocytes from Embryonic or Hematopoietic Stem Cells In Vitro
Abstract
INTRODUCTION: Differentiation of mouse embryonic stem cells (ESCs) or hematopoietic stem cells (HSCs) from fetal liver or bone marrow into T-lymphocytes can be achieved in vitro with the support of OP9-DL1 cells, a bone-marrow-derived stromal cell line that ectopically expresses the Notch ligand, Delta-like 1 (Dll1). This approach provides a simple, versatile, and efficient culture system that allows for the commitment, differentiation, and proliferation of T-lineage cells from different sources of stem cells. This article contains a series of protocols, the first of which describes the establishment, maintenance, and storage of OP9 and OP9-DL1 cells. Subsequent protocols detail how to co-culture the OP9 and OP9-DL1 cells with either ESCs or HSCs from fetal liver or bone marrow, leading to in vitro differentiation of the stem cells into lymphocytes.
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Citations
Cites
Induction of T Cell Development from Hematopoietic Progenitor Cells by Delta-like-1 In Vitro
Schmitt, T. M., & Zúñiga-Pflücker, J. C. (2002) · Immunity · doi:10.1016/s1074-7613(02)00474-0 · Cited by 1,120
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Metadata
- Authors
- Holmes, R., & Zúñiga-Pflücker, J. C.
- Venue
- Cold Spring Harbor Protocols
- Publisher
- Cold Spring Harbor Laboratory
- Published
- 2009-02
- Volume
- 2009
- Issue
- 2
- Pages
- pdb.prot5156
- ISSN
- 1559-6095 (ISSN-L)
- 1940-3402
- OpenAlex
- W2109350221
- Cited by
- 185
- References
- 1
- Type
- posted content
- Language
- en
- Open access
- closed
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- Holmes, R., & Zúñiga-Pflücker, J. C. (2009). The OP9-DL1 System: Generation of T-Lymphocytes from Embryonic or Hematopoietic Stem Cells In Vitro. Cold Spring Harbor Protocols. https://doi.org/10.1101/pdb.prot5156
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