The DOI matches its Crossref record; 10 of the 10 references it cites were found in a registry or an index.
Breaking Abbe’s diffraction resolution limit in fluorescence microscopy with stimulated emission depletion beams of various shapes
Abstract
We report on the generation of various hole-centered beams in the focal region of a lens and investigate their effectiveness to break the diffraction barrier in fluorescence microscopy by stimulated emission. Patterning of the phase of the stimulating beam across the entrance pupil of the objective lens produces point-spread-functions with twofold, fourfold, and circular symmetry, which narrow down the focal spot to 65-100 nm. Comparison with high-resolution confocal images exhibits a resolution much beyond the diffraction barrier. Particles that are only 65-nm apart are resolved with focused light.
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Cites
Principles of Fluorescence Spectroscopy
Lakowicz, J. R. (1999) · Springer eBooks · doi:10.1007/978-1-4757-3061-6 · Cited by 26,947
Principles of Optics: 60th Anniversary Edition
Born, M., & Wolf, E. (2019) · Cambridge University Press eBooks · doi:10.1017/9781108769914 · Cited by 26,251
Hell, S. W., & Wichmann, J. (1994) · Optics Letters · doi:10.1364/ol.19.000780 · Cited by 6,065
Self-citationRichards, B., & Wolf, E. (1959) · Proceedings of the Royal Society of London. Series A. Mathematical and Physical Sciences · doi:10.1098/rspa.1959.0200 · Cited by 3,095
Beiträge zur Theorie des Mikroskops und der mikroskopischen Wahrnehmung
Abbe, E. (1873) · Archiv für Mikroskopische Anatomie · doi:10.1007/bf02956173 · Cited by 2,724
Fluorescence microscopy with diffraction resolution barrier broken by stimulated emission
Klar, T. A., Jakobs, S., Dyba, M., Egner, A., & Hell, S. W. (2000) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.97.15.8206 · Cited by 1,869
Self-citationSubdiffraction resolution in far-field fluorescence microscopy
Klar, T. A., & Hell, S. W. (1999) · Optics Letters · doi:10.1364/ol.24.000954 · Cited by 864
Self-citationTopics in Fluorescence Spectroscopy: Principles
(2002) · Springer eBooks · doi:10.1007/b112907 · Cited by 589
Super-gain antennas and optical resolving power
Di Francia, G. T. (1952) · Il Nuovo Cimento · doi:10.1007/bf02903413 · Cited by 547
Generating radially polarized beams interferometrically
Tidwell, S. C., Ford, D. H., & Kimura, W. D. (1990) · Applied Optics · doi:10.1364/ao.29.002234 · Cited by 479
Cited by
Nanoscale Resolution in the Focal Plane of an Optical Microscope
Westphal, V., & Hell, S. W. (2005) · Physical Review Letters · doi:10.1103/physrevlett.94.143903 · Cited by 502
Self-citationSeeing beyond the limit: A guide to choosing the right super-resolution microscopy technique
Valli, J., Garcia-Burgos, A., Rooney, L. M., Vale de Melo e Oliveira, B., Duncan, R. R., & Rickman, C. (2021) · Journal of Biological Chemistry · doi:10.1016/j.jbc.2021.100791 · Cited by 159
Saffarian, S., & Kirchhausen, T. (2008) · Biophysical Journal · doi:10.1529/biophysj.107.117234 · Cited by 111
Metadata
- Authors
- Klar, T. A., Engel, E., & Hell, S. W.
- Venue
- Physical Review E
- Publisher
- American Physical Society (APS)
- Published
- 2001-11-26
- Volume
- 64
- Issue
- 6
- Pages
- 066613-066613
- ISSN
- 1063-651X (ISSN-L)
- 1095-3787
- OpenAlex
- W1972482793
- Cited by
- 276
- References
- 10
- FWCI
- 2.24
- Type
- journal article
- Language
- en
- Open access
- closed
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