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Focal Spots of Size λ / 23 Open Up Far-Field Florescence Microscopy at 33 nm Axial Resolution
Abstract
We report spots of excited molecules of 33 nm width created with focused light of lambda = 760 nm wavelength and conventional optics along the optic axis. This is accomplished by exciting the molecules with a femtosecond pulse and subsequent depletion of their excited state with red-shifted, picosecond-pulsed, counterpropagating, coherent light fields. The lambda/23 ratio constitutes what is to our knowledge the sharpest spatial definition attained with freely propagating electromagnetic radiation. The sub-diffraction spots enable for the first time far-field fluorescence microscopy with resolution at the tens of nanometer scale, as demonstrated in images of membranes of bacillus megaterium.
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Citations
Cited by
Hell, S. W. (2007) · Science · doi:10.1126/science.1137395 · Cited by 2,967
Self-citationSuper-resolution fluorescence microscopy
Huang, B., Bates, M., & Zhuang, X. (2009) · Annual Review of Biochemistry · doi:10.1146/annurev.biochem.77.061906.092014 · Cited by 1,657
A guide to super-resolution fluorescence microscopy
Schermelleh, L., Heintzmann, R., & Leonhardt, H. (2010) · Journal of Cell Biology · doi:10.1083/jcb.201002018 · Cited by 1,255
Development and Use of Fluorescent Protein Markers in Living Cells
Lippincott-Schwartz, J., & Patterson, G. H. (2003) · Science · doi:10.1126/science.1082520 · Cited by 992
Macromolecular-scale resolution in biological fluorescence microscopy
Donnert, G., Keller, J., Medda, R., Andrei, M. A., Rizzoli, S. O., Lührmann, R., Jahn, R., Eggeling, C., & Hell, S. W. (2006) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0604965103 · Cited by 527
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Metadata
- Authors
- Dyba, M., & Hell, S. W.
- Venue
- Physical Review Letters
- Publisher
- American Physical Society (APS)
- Published
- 2002-04-04
- Volume
- 88
- Issue
- 16
- Pages
- 163901-163901
- ISSN
- 0031-9007 (ISSN-L)
- 1079-7114
- OpenAlex
- W2050829557
- Cited by
- 364
- References
- 0
- FWCI
- 14.47
- Type
- journal article
- Language
- en
- Open access
- closed
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- Dyba, M., & Hell, S. W. (2002). Focal Spots of Size λ / 23 Open Up Far-Field Florescence Microscopy at 33 nm Axial Resolution. Physical Review Letters. https://doi.org/10.1103/physrevlett.88.163901
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