Overexpression of AtCpNifS Enhances Selenium Tolerance and Accumulation in Arabidopsis
Abstract
Selenium (Se) is an essential element for many organisms but is toxic at higher levels. CpNifS is a chloroplastic NifS-like protein in Arabidopsis (Arabidopsis thaliana) that can catalyze the conversion of cysteine into alanine and elemental sulfur (S0) and of selenocysteine into alanine and elemental Se (Se0). We overexpressed CpNifS to investigate the effects on Se metabolism in plants. CpNifS overexpression significantly enhanced selenate tolerance (1.9-fold) and Se accumulation (2.2-fold). CpNifS overexpressors showed significantly reduced Se incorporation into protein, which may explain their higher Se tolerance. Also, sulfur accumulation was enhanced by approximately 30% in CpNifS overexpressors, both on media with and without selenate. Root transcriptome changes in response to selenate mimicked the effects observed under sulfur starvation. There were only a few transcriptome differences between CpNifS-overexpressing plants and wild type, besides the 25- to 40-fold increase in CpNifS levels. Judged from x-ray analysis of near edge spectrum, both CpNifS overexpressors and wild type accumulated mostly selenate (SeVI). In conclusion, overexpression of this plant NifS-like protein had a pronounced effect on plant Se metabolism. The observed enhanced Se accumulation and tolerance of CpNifS overexpressors show promise for use in phytoremediation.
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The main form of Se accumulated by hyperaccumulator species is organic methyl-SeCys, while in related non-hyperaccumulators a large fraction of Se remains in the inorganic form of selenite.
“the main form of Se accumulated by hyperaccumulator species is organic methyl-SeCys [224], while in related non-hyperaccumulators a large fraction of the Se remains in the inorganic form of selenite [225,226].”
From Selenium Cycling Across Soil-Plant-Atmosphere Interfaces: A Critical Review (2015) · cites this paper as [226]
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References 45 · Cited by 151
- Authors
- Van Hoewyk, D., Garifullina, G. F., Ackley, A. R., Abdel-Ghany, S. E., Marcus, M. A., Fakra, S., Ishiyama, K., Inoue, E., Pilon, M., Takahashi, H., & Pilon-Smits, E. A. H.
- Affiliations
- Biology Department, Colorado State University, Fort Collins, Colorado 80523 (D.V.H., G.F.G., A.R.A., S.E.A.-G., M.P., E.A.H.P.-S.); Advanced Light Source, Lawrence Berkeley Laboratory, Berkeley, California 94720 (M.A.M., S.F.); and RIKEN Plant Science Center, Tsurumi-ku, Yokohama 230–0045, Japan (K.I., E.I., H.T.)
- Lawrence Berkeley National Laboratory (US)
- Venue
- Plant Physiology
- Publisher
- Oxford University Press (OUP)
- Published
- 2005-10-21
- Volume
- 139
- Issue
- 3
- Pages
- 1518-1528
- ISSN
- 0032-0889 (ISSN-L)
- 1532-2548
- OpenAlex
- W2148588871
- Cited by
- 151
- References
- 45
- Citations by year
- 2026: 3 · 2025: 6 · 2024: 4 · 2023: 11 · 2022: 12 · 2021: 4 · 2020: 29 · 2019: 4 · 2018: 9 · 2017: 9 · 2016: 4 · 2015: 7 · 2014: 3 · 2013: 3 · 2012: 7
- FWCI
- 5.81
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model (vor)
- Locations
- PLANT PHYSIOLOGY (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Science Foundation (DEAC02-05CH11231)
- U.S. Department of Energy (-AC02-05CH11231, 05CH11231, DE AC02 05CH11231, DE-AC02-)
- U.S. Department of Agriculture (DE-AC02-05CH11231)
- Office of Science (DEAC02–05CH11231, AC02-05CH11231, DE-AC02)
- Japan Society for the Promotion of Science
- Basic Energy Sciences (DE-AC02, DE-AC02-05CH11231., AC02–05CH11231)
- Lawrence Berkeley National Laboratory (AC02‐05CH11231, DE-AC02-05CH11231, 05CH11231)
- Topics
- Health Sciences › Nursing › Nutrition and Dietetics › Selenium in Biological Systems
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Nitrogen and Sulfur Effects on Brassica
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Micronutrient Interactions and Effects
- Keywords
- selenium tolerance, selenium accumulation, selenate, selenocysteine, sulfur metabolism, elemental selenium, root transcriptome, sulfur deficiency, phytoremediation, Arabidopsis thaliana
- MeSH terms
- Selenium, Sulfur, Transcription, Genetic, Gene Expression, Arabidopsis, Gene Expression Regulation, Plant, Plant Roots, Plant Shoots, Oligonucleotide Array Sequence Analysis, Gene Expression Profiling, Arabidopsis Proteins, Plants, Genetically Modified, Seedlings
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