The DOI matches its Crossref record; 16 of the 16 references it cites were found in a registry or an index.
Dissection of the AtNRT2.1:AtNRT2.2 Inducible High-Affinity Nitrate Transporter Gene Cluster
Open accessAbstract
Using a new Arabidopsis (Arabidopsis thaliana) mutant (Atnrt2.1-nrt2.2) we confirm that concomitant disruption of NRT2.1 and NRT2.2 reduces inducible high-affinity transport system (IHATS) by up to 80%, whereas the constitutive high-affinity transport system (CHATS) was reduced by 30%. Nitrate influx via the low-affinity transport system (LATS) was unaffected. Shoot-to-root ratios were significantly reduced compared to wild-type plants, the major effect being upon shoot growth. In another mutant uniquely disrupted in NRT2.1 (Atnrt2.1), IHATS was reduced by up to 72%, whereas neither the CHATS nor the LATS fluxes were significantly reduced. Disruption of NRT2.1 in Atnrt2.1 caused a consistent and significant reduction of shoot-to-root ratios. IHATS influx and shoot-to-root ratios were restored to wild-type values when Atnrt2.1-nrt2.2 was transformed with a NRT2.1 cDNA isolated from Arabidopsis. Disruption of NRT2.2 in Atnrt2.2 reduced IHATS by 19% and this reduction was statistically significant only at 6 h after resupply of nitrate to nitrogen-deprived plants. Atnrt2.2 showed no significant reduction of CHATS, LATS, or shoot-to-root ratios. These results define NRT2.1 as the major contributor to IHATS. Nevertheless, when maintained on agar containing 0.25 mm KNO(3) as the sole nitrogen source, Atnrt2.1-nrt2.2 consistently exhibited greater stress and growth reduction than Atnrt2.1. Evidence from real-time PCR revealed that NRT2.2 transcript abundance was increased almost 3-fold in Atnrt2.1. These findings suggest that NRT2.2 normally makes only a small contribution to IHATS, but when NRT2.1 is lost, this contribution increases, resulting in a partial compensation.
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Cites
Molecular and physiological aspects of nitrate uptake in plants
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Self-citationNitrate transporters in plants: structure, function and regulation
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Studies of the Uptake of Nitrate in Barley: I. Kinetics of 13NO3− Influx
Siddiqi, M. Y., Glass, A. D. M., Ruth, T. J., & Rufty, T. W. (1990) · Plant Physiology · doi:10.1104/pp.93.4.1426 · Cited by 378
Self-citationRegulation of NRT1 and NRT2 Gene Families of Arabidopsis thaliana: Responses to Nitrate Provision
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Self-citationAn Arabidopsis T‐DNA mutant affected in Nrt2 genes is impaired in nitrate uptake
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Analysis of the NRT2 Nitrate Transporter Family in Arabidopsis. Structure and Gene Expression
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The Arabidopsis CHL1 protein plays a major role in high-affinity nitrate uptake
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Self-citationVidmar, J. J., Zhuo, D., Siddiqi, M. Y., Schjoerring, J. K., Touraine, B., & Glass, A. D. M. (2000) · Plant Physiology · doi:10.1104/pp.123.1.307 · Cited by 237
Self-citationcrnA encodes a nitrate transporter in Aspergillus nidulans.
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Cited by
Source and sink mechanisms of nitrogen transport and use
Tegeder, M., & Masclaux‐Daubresse, C. (2018) · New Phytologist · doi:10.1111/nph.14876 · Cited by 869
Nitrate transporters and peptide transporters
Tsay, Y.-F., Chiu, C.-C., Tsai, C.-B., Ho, C.-H., & Hsu, P.-K. (2007) · FEBS Letters · doi:10.1016/j.febslet.2007.04.047 · Cited by 577
Nitrate transport and signalling
Miller, A. J., Fan, X., Orsel, M., Smith, S. J., & Wells, D. M. (2007) · Journal of Experimental Botany · doi:10.1093/jxb/erm066 · Cited by 526
Li, J.-Y., Fu, Y.-L., Pike, S. M., Bao, J., Tian, W., Zhang, Y., Chen, C.-Z., Zhang, Y., Li, H.-M., Huang, J., et al. (2010) · The Plant Cell · doi:10.1105/tpc.110.075242 · Cited by 441
Kiba, T., Feria-Bourrellier, A.-B., Lafouge, F., Lezhneva, L., Boutet-Mercey, S., Orsel, M., Bréhaut, V., Miller, A., Daniel-Vedele, F., Sakakibara, H., et al. (2012) · The Plant Cell · doi:10.1105/tpc.111.092221 · Cited by 422
Metadata
- Authors
- Li, W., Wang, Y., Okamoto, M., Crawford, N. M., Siddiqi, M. Y., & Glass, A. D. M.
- Venue
- Plant Physiology
- Publisher
- Oxford University Press (OUP)
- Published
- 2006-11-03
- Volume
- 143
- Issue
- 1
- Pages
- 425-433
- ISSN
- 0032-0889 (ISSN-L)
- 1532-2548
- OpenAlex
- W2069003130
- Cited by
- 366
- References
- 16
- FWCI
- 8.52
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Li, W., Wang, Y., Okamoto, M., Crawford, N. M., Siddiqi, M. Y., & Glass, A. D. M. (2007). Dissection of the AtNRT2.1:AtNRT2.2 Inducible High-Affinity Nitrate Transporter Gene Cluster. Plant Physiology. https://doi.org/10.1104/pp.106.091223
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