The DOI matches its Crossref record; 20 of the 20 references it cites were found in a registry or an index.
An Arabidopsis T‐DNA mutant affected in Nrt2 genes is impaired in nitrate uptake
Abstract
Expression analyses of Nrt2 plant genes have shown a strict correlation with root nitrate influx mediated by the high‐affinity transport system (HATS). The precise assignment of NRT2 protein function has not yet been possible due to the absence of heterologous expression studies as well as loss of function mutants in higher plants. Using a reverse genetic approach, we isolated an Arabidopsis thaliana knock‐out mutant where the T‐DNA insertion led to the complete deletion of the AtNrt2.1 gene together with the deletion of the 3′ region of the AtNrt2.2 gene. This mutant is impaired in the HATS, without being modified in the low‐affinity system. Moreover, the de‐regulated expression of a Nicotiana plumbaginifolia Nrt2 gene restored the mutant nitrate influx to that of the wild‐type. These results demonstrate that plant NRT2 proteins do have a role in HATS.
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Cited by
Lezhneva, L., Kiba, T., Feria‐Bourrellier, A., Lafouge, F., Boutet‐Mercey, S., Zoufan, P., Sakakibara, H., Daniel‐Vedele, F., & Krapp, A. (2014) · The Plant Journal · doi:10.1111/tpj.12626 · Cited by 369
Self-citationInorganic nitrogen assimilation in Chlamydomonas
Fernandez, E., & Galvan, A. (2007) · Journal of Experimental Botany · doi:10.1093/jxb/erm106 · Cited by 177
The mutational consequences of plant transformation
Latham, J. R., Wilson, A. K., & Steinbrecher, R. A. (2006) · BioMed Research International · doi:10.1155/jbb/2006/25376 · Cited by 155
Transformation-induced mutations in transgenic plants: analysis and biosafety implications
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Chen, Y., Li, Y., Fu, Y., Jia, L., Li, L., Xu, Z., Zhang, N., Liu, Y., Fan, X., Xuan, W., et al. (2024) · Journal of Experimental Botany · doi:10.1093/jxb/erae125 · Cited by 31
Metadata
- Authors
- Filleur, S., Dorbe, M.-F., Cerezo, M., Orsel, M., Granier, F., Gojon, A., & Daniel-Vedele, F.
- Venue
- FEBS Letters
- Publisher
- Wiley
- Published
- 2001-01-31
- Volume
- 489
- Issue
- 2-3
- Pages
- 220-224
- ISSN
- 0014-5793 (ISSN-L)
- 1873-3468
- OpenAlex
- W2066314387
- Cited by
- 323
- References
- 20
- FWCI
- 12.80
- Type
- journal article
- Language
- en
- Open access
- closed
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- Filleur, S., Dorbe, M.-F., Cerezo, M., Orsel, M., Granier, F., Gojon, A., & Daniel-Vedele, F. (2001). An Arabidopsis T‐DNA mutant affected in Nrt2 genes is impaired in nitrate uptake. FEBS Letters. https://doi.org/10.1016/s0014-5793(01)02096-8
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