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Pathogenesis-Related PR-1 Proteins Are Antifungal (Isolation and Characterization of Three 14-Kilodalton Proteins of Tomato and of a Basic PR-1 of Tobacco with Inhibitory Activity against Phytophthora infestans)

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Niderman, T., Genetet, I., Bruyere, T., Gees, R., Stintzi, A., Legrand, M., Fritig, B., Mosinger, E.·1995·Plant Physiology

Abstract

Three distinct basic 14-kD proteins, P14a, P14b, and P14c, were isolated from tomato (Lycopersicon esculentum Mill. cv Baby) leaves infected with Phytophthora infestans. They exhibited antifungal activity against P. infestans both in vitro (inhibition of zoospore germination) and in vivo with a tomato leaf disc assay (decrease in infected leaf surface). Serological cross-reactions and amino acid sequence comparisons showed that the three proteins are members of the PR-1 group of pathogenesis-related (PR) proteins. P14a and P14b showed high similarity to a previously characterized P14, whereas P14c was found to be very similar to a putative basic-type PR-1 from tobacco predicted from isolated DNA clones. This protein, named PR-1 g, was purified from virus-infected tobacco (Nicotiana tabacum Samsun NN) leaves and characterized by amino acid microsequencing, along with the well-known acidic tobacco PR-1a, PR-1b, and PR-1c. The various tomato and tobacco PR-1 proteins were compared for their biological activity and found to display differential fungicidal activity against P. infestans in both the in vitro and in vivo assays, the most efficient being the newly characterized tomato P14c and tobacco PR-1g.

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Metadata

Authors
Niderman, T., Genetet, I., Bruyere, T., Gees, R., Stintzi, A., Legrand, M., Fritig, B., & Mosinger, E.
Venue
Plant Physiology
Published
1995-05-01
Volume
108
Issue
1
Pages
17-27
ISSN
  • 0032-0889 (ISSN-L)
  • 1532-2548
OpenAlex
W2050352895
Cited by
469
References
2
FWCI
18.67
Type
journal article
Language
en
Open access
green

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CoChat. (2026). Citation check: Pathogenesis-Related PR-1 Proteins Are Antifungal (Isolation and Characterization of Three 14-Kilodalton Proteins of Tomato and of a Basic PR-1 of Tobacco with Inhibitory Activity against Phytophthora infestans). https://app.cochat.ai/papers/10.1104/pp.108.1.17
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Niderman, T., Genetet, I., Bruyere, T., Gees, R., Stintzi, A., Legrand, M., Fritig, B., & Mosinger, E. (1995). Pathogenesis-Related PR-1 Proteins Are Antifungal (Isolation and Characterization of Three 14-Kilodalton Proteins of Tomato and of a Basic PR-1 of Tobacco with Inhibitory Activity against Phytophthora infestans). Plant Physiology. https://doi.org/10.1104/pp.108.1.17

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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).

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