The DOI matches its Crossref record; 42 of the 42 references it cites were found in a registry or an index.
Inhibition of SNF1-Related Protein Kinase1 Activity and Regulation of Metabolic Pathways by Trehalose-6-Phosphate
Open accessAbstract
Trehalose-6-phosphate (T6P) is a proposed signaling molecule in plants, yet how it signals was not clear. Here, we provide evidence that T6P functions as an inhibitor of SNF1-related protein kinase1 (SnRK1; AKIN10/AKIN11) of the SNF1-related group of protein kinases. T6P, but not other sugars and sugar phosphates, inhibited SnRK1 in Arabidopsis (Arabidopsis thaliana) seedling extracts strongly (50%) at low concentrations (1–20 μ m). Inhibition was noncompetitive with respect to ATP. In immunoprecipitation studies using antibodies to AKIN10 and AKIN11, SnRK1 catalytic activity and T6P inhibition were physically separable, with T6P inhibition of SnRK1 dependent on an intermediary factor. In subsequent analysis, T6P inhibited SnRK1 in extracts of all tissues analyzed except those of mature leaves, which did not contain the intermediary factor. To assess the impact of T6P inhibition of SnRK1 in vivo, gene expression was determined in seedlings expressing Escherichia coli otsA encoding T6P synthase to elevate T6P or otsB encoding T6P phosphatase to decrease T6P. SnRK1 target genes showed opposite regulation, consistent with the regulation of SnRK1 by T6P in vivo. Analysis of microarray data showed up-regulation by T6P of genes involved in biosynthetic reactions, such as genes for amino acid, protein, and nucleotide synthesis, the tricarboxylic acid cycle, and mitochondrial electron transport, which are normally down-regulated by SnRK1. In contrast, genes involved in photosynthesis and degradation processes, which are normally up-regulated by SnRK1, were down-regulated by T6P. These experiments provide strong evidence that T6P inhibits SnRK1 to activate biosynthetic processes in growing tissues.
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Cites
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AMP-activated/SNF1 protein kinases: conserved guardians of cellular energy
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A central integrator of transcription networks in plant stress and energy signalling
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Trehalose accumulation in rice plants confers high tolerance levels to different abiotic stresses
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A Gene Expression Map of the Arabidopsis Root
Birnbaum, K., Shasha, D. E., Wang, J. Y., Jung, J. W., Lambert, G. M., Galbraith, D. W., & Benfey, P. N. (2003) · Science · doi:10.1126/science.1090022 · Cited by 1,258
A High-Resolution Root Spatiotemporal Map Reveals Dominant Expression Patterns
Brady, S. M., Orlando, D. A., Lee, J.-Y., Wang, J. Y., Koch, J., Dinneny, J. R., Mace, D., Ohler, U., & Benfey, P. N. (2007) · Science · doi:10.1126/science.1146265 · Cited by 1,182
Role of the Arabidopsis Glucose Sensor HXK1 in Nutrient, Light, and Hormonal Signaling
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Trehalose Metabolism and Signaling
Paul, M. J., Primavesi, L. F., Jhurreea, D., & Zhang, Y. (2008) · Annual Review of Plant Biology · doi:10.1146/annurev.arplant.59.032607.092945 · Cited by 723
Self-citationUsadel, B., Nagel, A., Thimm, O., Redestig, H., Blaesing, O. E., Palacios-Rojas, N., Selbig, J., Hannemann, J., Piques, M. C., Steinhauser, D., et al. (2005) · Plant Physiology · doi:10.1104/pp.105.060459 · Cited by 618
Cited by
Slama, I., Abdelly, C., Bouchereau, A., Flowers, T., & Savouré, A. (2015) · Annals of Botany · doi:10.1093/aob/mcu239 · Cited by 937
Sugar signals and molecular networks controlling plant growth
Smeekens, S., Ma, J., Hanson, J., & Rolland, F. (2010) · Current Opinion in Plant Biology · doi:10.1016/j.pbi.2009.12.002 · Cited by 655
Trehalose metabolism in plants
Lunn, J. E., Delorge, I., Figueroa, C. M., Van Dijck, P., & Stitt, M. (2014) · The Plant Journal · doi:10.1111/tpj.12509 · Cited by 623
Braun, D. M., Wang, L., & Ruan, Y.-L. (2014) · Journal of Experimental Botany · doi:10.1093/jxb/ert416 · Cited by 482
Nuccio, M. L., Wu, J., Mowers, R., Zhou, H.-P., Meghji, M., Primavesi, L. F., Paul, M. J., Chen, X., Gao, Y., Haque, E., et al. (2015) · Nature Biotechnology · doi:10.1038/nbt.3277 · Cited by 473
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Metadata
- Authors
- Zhang, Y., Primavesi, L. F., Jhurreea, D., Andralojc, P. J., Mitchell, R. A. C., Powers, S. J., Schluepmann, H., Delatte, T., Wingler, A., & Paul, M. J.
- Venue
- Plant Physiology
- Publisher
- Oxford University Press (OUP)
- Published
- 2009-02-04
- Volume
- 149
- Issue
- 4
- Pages
- 1860-1871
- ISSN
- 0032-0889 (ISSN-L)
- 1532-2548
- OpenAlex
- W2090189626
- Cited by
- 600
- References
- 42
- FWCI
- 31.74
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Zhang, Y., Primavesi, L. F., Jhurreea, D., Andralojc, P. J., Mitchell, R. A. C., Powers, S. J., Schluepmann, H., Delatte, T., Wingler, A., & Paul, M. J. (2009). Inhibition of SNF1-Related Protein Kinase1 Activity and Regulation of Metabolic Pathways by Trehalose-6-Phosphate. Plant Physiology. https://doi.org/10.1104/pp.108.133934
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