The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.
Overexpression of [delta]-Pyrroline-5-Carboxylate Synthetase Increases Proline Production and Confers Osmotolerance in Transgenic Plants
Open accessAbstract
Proline (Pro) accumulation has been correlated with tolerance to drought and salinity stresses in plants. Therefore, overproduction of Pro in plants may lead to increased tolerance against these abiotic stresses. To test this possibility, we overexpressed in tobacco the mothbean [delta]-pyrroline-5-carboxylate synthetase, a bifunctional enzyme able to catalyze the conversion of glutamate to [delta]-pyrroline-5-carboxylate, which is then reduced to Pro. The transgenic plants produced a high level of the enzyme and synthesized 10- to 18-fold more Pro than control plants. These results suggest that activity of the first enzyme of the pathway is the rate-limiting factor in Pro synthesis. Exogenous supply of nitrogen further enhanced Pro production. The osmotic potentials of leaf sap from transgenic plants were less decreased under water-stress conditions compared to those of control plants. Overproduction of Pro also enhanced root biomass and flower development in transgenic plants under drought-stress conditions. These data demonstrated that Pro acts as an osmoprotectant and that overproduction of Pro results in the increased tolerance to osmotic stress in plants.
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Cites
Rapid determination of free proline for water-stress studies
Bates, L. S., Waldren, R. P., & Teare, I. D. (1973) · Plant and Soil · doi:10.1007/bf00018060 · Cited by 20,967
Plant productivity and environment
Boyer, J. S. (1982) · Science · doi:10.1126/science.218.4571.443 · Cited by 3,959
Cited by
Kasuga, M., Liu, Q., Miura, S., Yamaguchi-Shinozaki, K., & Shinozaki, K. (1999) · Nature Biotechnology · doi:10.1038/7036 · Cited by 2,119
The interaction of plant biotic and abiotic stresses: from genes to the field
Atkinson, N. J., & Urwin, P. E. (2012) · Journal of Experimental Botany · doi:10.1093/jxb/ers100 · Cited by 1,880
Arbuscular mycorrhizal fungi in alleviation of salt stress: a review
Evelin, H., Kapoor, R., & Giri, B. (2009) · Annals of Botany · doi:10.1093/aob/mcp251 · Cited by 1,083
Calcium signalling in Arabidopsis thaliana responding to drought and salinity
Knight, H., Trewavas, A. J., & Knight, M. R. (1997) · The Plant Journal · doi:10.1046/j.1365-313x.1997.12051067.x · Cited by 915
Polyamines and abiotic stress: recent advances
Groppa, M. D., & Benavides, M. P. (2008) · Amino Acids · doi:10.1007/s00726-007-0501-8 · Cited by 788
Metadata
- Authors
- Kishor, P., Hong, Z., Miao, G. H., Hu, C., & Verma, D.
- Venue
- Plant Physiology
- Publisher
- Oxford University Press (OUP)
- Published
- 1995-08-01
- Volume
- 108
- Issue
- 4
- Pages
- 1387-1394
- ISSN
- 0032-0889 (ISSN-L)
- 1532-2548
- OpenAlex
- W1832710699
- Cited by
- 1,351
- References
- 2
- FWCI
- 44.92
- Type
- journal article
- Language
- en
- Open access
- green
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- Kishor, P., Hong, Z., Miao, G. H., Hu, C., & Verma, D. (1995). Overexpression of [delta]-Pyrroline-5-Carboxylate Synthetase Increases Proline Production and Confers Osmotolerance in Transgenic Plants. Plant Physiology. https://doi.org/10.1104/pp.108.4.1387
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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