Involvement of Silicon Influx Transporter OsNIP2;1 in Selenite Uptake in Rice
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Rice (Oryza sativa) as a staple food, provides a major source of dietary selenium (Se) for humans, which essentially requires Se, however, the molecular mechanism for Se uptake is still poorly understood. Herein, we show evidence that the uptake of selenite, a main bioavailable form of Se in paddy soils, is mediated by a silicon (Si) influx transporter Lsi1 (OsNIP2;1) in rice. Defect of OsNIP2;1 resulted in a significant decrease in the Se concentration of the shoots and xylem sap when selenite was given. However, there was no difference in the Se concentration between the wild-type rice and mutant of OsNIP2;1 when selenate was supplied. A short-term uptake experiment showed that selenite uptake greatly increased with decreasing pH in the external solution. Si as silicic acid did not inhibit the Se uptake from selenite in both rice and yeast (Saccharomyces cerevisiae) at low pHs. Expression of OsNIP2;1 in yeast enhanced the selenite uptake at pH 3.5 and 5.5 but not at pH 7.5. On the other hand, defect of Si efflux transporter Lsi2 did not affect the uptake of Se either from selenite or selenate. Taken together, our results indicate that Si influx transporter OsNIP2;1 is permeable to selenite.
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Selenite may possibly be internalized via Si-transporters in rice.
“and possibly Si-transporters (e.g in rice [177].”
From Selenium Cycling Across Soil-Plant-Atmosphere Interfaces: A Critical Review (2015) · cites this paper as [177]
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- Authors
- Zhao, X. Q., Mitani, N., Yamaji, N., Shen, R. F., & Ma, J. F.
- Affiliations
- Okayama University (JP)
- State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, 210008 Nanjing, China (X.Q.Z., R.F.S.); Institute of Plant Science and Resources, Okayama University, Kurashiki 710–0046, Japan (N.M., N.Y., J.F.M.)
- Corresponding author
- Shen, Ren Fang
- Venue
- Plant Physiology
- Publisher
- Oxford University Press (OUP)
- Published
- 2010-05-24
- Volume
- 153
- Issue
- 4
- Pages
- 1871-1877
- ISSN
- 0032-0889 (ISSN-L)
- 1532-2548
- PMC
- PMC2923891
- OpenAlex
- W2100493050
- Cited by
- 320
- References
- 34
- Citations by year
- 2026: 15 · 2025: 30 · 2024: 24 · 2023: 33 · 2022: 31 · 2021: 30 · 2020: 39 · 2019: 18 · 2018: 18 · 2017: 20 · 2016: 23 · 2015: 15 · 2014: 4 · 2013: 12 · 2012: 2
- FWCI
- 7.70
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Locations
- PLANT PHYSIOLOGY (publishedVersion, cc-by)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Greater South Information System
- Funders
- Jiangsu Academy of Agricultural Sciences
- Ministry of Education, Culture, Sports, Science and Technology
- Chinese Academy of Sciences
- Ministry of Agriculture, Forestry and Fisheries
- Institute of Soil Science, Chinese Academy of Sciences
- Topics
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Silicon Effects in Agriculture
- Health Sciences › Nursing › Nutrition and Dietetics › Selenium in Biological Systems
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant nutrient uptake and metabolism
- Keywords
- selenite uptake, rice, selenium, Lsi1, Lsi2, selenate, pH dependence, silicic acid, xylem sap, yeast complementation
- MeSH terms
- Hydrogen-Ion Concentration, Mutation, Plant Proteins, Oryza, Silicic Acid, Selenium Compounds, Sodium Selenite, Aquaporins, Selenic Acid
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