The DOI matches its Crossref record; 38 of the 38 references it cites were found in a registry or an index.
Functional Characterization of Evolutionarily Divergent 4-Coumarate:Coenzyme A Ligases in Rice
Open accessAbstract
4-Coumarate:coenzyme A ligase (4CL; EC 6.2.1.12) is a key enzyme in the phenylpropanoid metabolic pathways for monolignol and flavonoid biosynthesis. 4CL has been much studied in dicotyledons, but its function is not completely understood in monocotyledons, which display a different monolignol composition and phenylpropanoid profile. In this study, five members of the 4CL gene family in the rice (Oryza sativa) genome were cloned and analyzed. Biochemical characterization of the 4CL recombinant proteins revealed that the rice 4CL isoforms displayed different substrate specificities and catalytic turnover rates. Among them, Os4CL3 exhibited the highest turnover rate. No apparent tissue-specific expression of the five 4CLs was observed, but significant differences in their expression levels were detected. The rank in order of transcript abundance was Os4CL3 > Os4CL5 > Os4CL1 > Os4CL4 > Os4CL2. Suppression of Os4CL3 expression resulted in significant lignin reduction, shorter plant growth, and other morphological changes. The 4CL-suppressed transgenics also displayed decreased panicle fertility, which may be attributed to abnormal anther development as a result of disrupted lignin synthesis. This study demonstrates that the rice 4CLs exhibit different in vitro catalytic properties from those in dicots and that 4CL-mediated metabolism in vivo may play important roles in regulating a broad range of biological events over the course of rice growth and development.
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Self-citationGenome-wide analysis of phenylpropanoid defence pathways.
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BRITTLE CULM1, Which Encodes a COBRA-Like Protein, Affects the Mechanical Properties of Rice Plants
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Cited by
Contribution of phenylpropanoid metabolism to plant development and plant–environment interactions
Dong, N., & Lin, H. (2021) · Journal of Integrative Plant Biology · doi:10.1111/jipb.13054 · Cited by 1,748
The Role of Hydroxycinnamic Acid Amide Pathway in Plant Immunity
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Early phenylpropanoid biosynthetic steps in Cannabis sativa: link between genes and metabolites.
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Metadata
- Authors
- Gui, J., Shen, J., & Li, L.
- Venue
- Plant Physiology
- Publisher
- Oxford University Press (OUP)
- Published
- 2011-08-01
- Volume
- 157
- Issue
- 2
- Pages
- 574-586
- ISSN
- 0032-0889 (ISSN-L)
- 1532-2548
- OpenAlex
- W2163992044
- Cited by
- 304
- References
- 38
- FWCI
- 3.64
- Type
- journal article
- Language
- en
- Open access
- hybrid
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