Cited by 120Plant Stress Responses and ToleranceSeed Germination and PhysiologyPlant nutrient uptake and metabolism

The Arabidopsis bZIP Gene AtbZIP63 Is a Sensitive Integrator of Transient Abscisic Acid and Glucose Signals

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Matiolli, Cleverson Carlos, Tomaz, Juarez Pires, Duarte, Gustavo Turqueto, Prado, Fernanda Manso, Del Bem, Luiz Eduardo Vieira, Silveira, Amanda Bortolini, Gauer, Luciane, Corrêa, Luiz Gustavo Guedes +5 more·2011·Plant Physiology

Abstract

Glucose modulates plant metabolism, growth, and development. In Arabidopsis (Arabidopsis thaliana), Hexokinase1 (HXK1) is a glucose sensor that may trigger abscisic acid (ABA) synthesis and sensitivity to mediate glucose-induced inhibition of seedling development. Here, we show that the intensity of short-term responses to glucose can vary with ABA activity. We report that the transient (2 h/4 h) repression by 2% glucose of AtbZIP63, a gene encoding a basic-leucine zipper (bZIP) transcription factor partially involved in the Snf1-related kinase KIN10-induced responses to energy limitation, is independent of HXK1 and is not mediated by changes in ABA levels. However, high-concentration (6%) glucose-mediated repression appears to be modulated by ABA, since full repression of AtbZIP63 requires a functional ABA biosynthetic pathway. Furthermore, the combination of glucose and ABA was able to trigger a synergistic repression of AtbZIP63 and its homologue AtbZIP3, revealing a shared regulatory feature consisting of the modulation of glucose sensitivity by ABA. The synergistic regulation of AtbZIP63 was not reproduced by an AtbZIP63 promoter-5′-untranslated region::β-glucuronidase fusion, thus suggesting possible posttranscriptional control. A transcriptional inhibition assay with cordycepin provided further evidence for the regulation of mRNA decay in response to glucose plus ABA. Overall, these results indicate that AtbZIP63 is an important node of the glucose-ABA interaction network. The mechanisms by which AtbZIP63 may participate in the fine-tuning of ABA-mediated abiotic stress responses according to sugar availability (i.e., energy status) are discussed.

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Crossref · 10.1104/pp.111.181743

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Authors
Matiolli, C. C., Tomaz, J. P., Duarte, G. T., Prado, F. M., Del Bem, L. E. V., Silveira, A. B., Gauer, L., Corrêa, L. G. G., Drumond, R. D., Viana, A. J. C., Di Mascio, P., Meyer, C., & Vincentz, M.
Affiliations
  • Universidade Estadual de Campinas (UNICAMP) (BR)
  • Centro de Biologia Molecular e Engenharia Genética, Universidade Estadual de Campinas, Cidade Universitária Zeferino Vaz, CP6010 Campinas, Sao Paulo, Brazil (C.C.M., J.P.T., G.T.D., L.E.V.D.B., A.B.S., L.G., L.G.G.C., R.D.D., A.J.C.V., M.V.); Departamento de Biologia Vegetal, Instituto de Biologia, Cidade Universitária Zeferino Vaz, CP6009 Campinas, Sao Paulo, Brazil (M.V.); Unité de Nutrition Az
Corresponding author
Vincentz, Michel
Venue
Plant Physiology
Published
2011-08-15
Volume
157
Issue
2
Pages
692-705
ISSN
  • 0032-0889 (ISSN-L)
  • 1532-2548
OpenAlex
W2066752940
Cited by
120
References
99
Citations by year
2026: 5 · 2025: 6 · 2024: 6 · 2023: 10 · 2022: 9 · 2021: 10 · 2020: 7 · 2019: 9 · 2018: 12 · 2017: 8 · 2016: 6 · 2015: 8 · 2014: 11 · 2013: 4 · 2012: 7
FWCI
9.22
Type
journal article
Language
en
Open access
Locations
  • PLANT PHYSIOLOGY (publishedVersion, cc-by)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
  • Scientific Electronic Library Online (São Paulo Research Foundation, Latin American and Caribbean Center on Health Sciences Information, Conselho Nacional de Desenvolvimento Científico e Tecnológico) (publishedVersion)
  • HAL (submittedVersion)
Topics
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Stress Responses and Tolerance
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Seed Germination and Physiology
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant nutrient uptake and metabolism
Keywords
abscisic acid, glucose signaling, bZIP transcription factors, transcriptional repression, mRNA degradation, post-transcriptional regulation, energy limitation, abiotic stress response, sugar availability, Arabidopsis thaliana
MeSH terms
Abscisic Acid, Glucose, Glucuronidase, Hexokinase, Promoter Regions, Genetic, Signal Transduction, Trans-Activators, Protein Serine-Threonine Kinases, Gene Expression Regulation, Plant, 5' Untranslated Regions, RNA Stability, Arabidopsis Proteins, Basic-Leucine Zipper Transcription Factors, Biosynthetic Pathways

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