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Evidence for the Presence of the Ascorbate-Glutathione Cycle in Mitochondria and Peroxisomes of Pea Leaves
Open accessAbstract
The presence of the enzymes of the ascorbate-glutathione cycle was investigated in mitochondria and peroxisomes purified from pea (Pisum sativum L.) leaves. All four enzymes, ascorbate peroxidase (APX; EC 1.11.1.11), monodehydroascorbate reductase (EC 1.6.5.4), dehydroascorbate reductase (EC 1.8.5.1), and glutathione reductase (EC 1.6.4.2), were present in mitochondria and peroxisomes, as well as in the antioxidants ascorbate and glutathione. The activity of the ascorbate-glutathione cycle enzymes was higher in mitochondria than in peroxisomes, except for APX, which was more active in peroxisomes than in mitochondria. Intact mitochondria and peroxisomes had no latent APX activity, and this remained in the membrane fraction after solubilization assays with 0.2 M KCl. Monodehydroascorbate reductase was highly latent in intact mitochondria and peroxisomes and was membrane-bound, suggesting that the electron acceptor and donor sites of this redox protein are not on the external side of the mitochondrial and peroxisomal membranes. Dehydroascorbate reductase was found mainly in the soluble peroxisomal and mitochondrial fractions. Glutathione reductase had a high latency in mitochondria and peroxisomes and was present in the soluble fractions of both organelles. In intact peroxisomes and mitochondria, the presence of reduced ascorbate and glutathione and the oxidized forms of ascorbate and glutathione were demonstrated by high-performance liquid chromatography analysis. The ascorbate-glutathione cycle of mitochondria and peroxisomes could represent an important antioxidant protection system against H2O2 generated in both plant organelles.
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Cites
Hydrogen Peroxide is Scavenged by Ascorbate-specific Peroxidase in Spinach Chloroplasts
Nakano, Y., & Asada, K. (1981) · Plant and Cell Physiology · doi:10.1093/oxfordjournals.pcp.a076232 · Cited by 10,809
Cited by
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Foyer, C. H., & Noctor, G. (2003) · Physiologia Plantarum · doi:10.1034/j.1399-3054.2003.00223.x · Cited by 1,316
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Ashraf, M. (2009) · Biotechnology Advances · doi:10.1016/j.biotechadv.2008.09.003 · Cited by 1,137
Hernández, J. A., Jiménez, A., Mullineaux, P., & Sevilia, F. (2000) · Plant, Cell & Environment · doi:10.1046/j.1365-3040.2000.00602.x · Cited by 837
Self-citationShalata, A., Mittova, V., Volokita, M., Guy, M., & Tal, M. (2001) · Physiologia Plantarum · doi:10.1034/j.1399-3054.2001.1120405.x · Cited by 448
Metadata
- Authors
- Jimenez, A., Hernandez, J. A., del Rio, L. A., & Sevilla, F.
- Venue
- Plant Physiology
- Publisher
- Oxford University Press (OUP)
- Published
- 1997-05-01
- Volume
- 114
- Issue
- 1
- Pages
- 275-284
- ISSN
- 0032-0889 (ISSN-L)
- 1532-2548
- OpenAlex
- W2147533928
- Cited by
- 838
- References
- 1
- FWCI
- 6.08
- Type
- journal article
- Language
- en
- Open access
- green
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- Jimenez, A., Hernandez, J. A., del Rio, L. A., & Sevilla, F. (1997). Evidence for the Presence of the Ascorbate-Glutathione Cycle in Mitochondria and Peroxisomes of Pea Leaves. Plant Physiology. https://doi.org/10.1104/pp.114.1.275
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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