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Ascorbate Biosynthesis in Mitochondria Is Linked to the Electron Transport Chain between Complexes III and IV
Open accessAbstract
Ascorbic acid is synthesized from galactono-γ-lactone (GL) in plant tissues. An improved extraction procedure involving ammonium sulfate precipitation of membrane proteins from crude leaf homogenates yielded a simple, quick method for determining tissue activities of galactono-γ-lactone dehydrogenase (GLDH). Total foliar ascorbate and GLDH activity decreased with leaf age. Subcellular fractionation experiments using marker enzymes demonstrated that 80% of the total GLDH activity was located on the inner mitochondrial membrane, and 20% in the microsomal fraction. Specific antibody raised against potato (Solanum tuberosum L.) tuber GLDH recognized a 56-kD polypeptide in extracts from the mitochondrial membranes but failed to detect the equivalent polypeptide in microsomes. We demonstrate that isolated intact mitochondria synthesize ascorbate in the presence of GL. GL stimulated mitochondrial electron transport rates. The respiration inhibitor antimycin A stimulated ascorbate biosynthesis, while cyanide inhibited both respiration and ascorbate production. GL-dependent oxygen uptake was observed in isolated intact mitochondria. This evidence suggests that GLDH delivers electrons to the mitochondrial electron transport chain between complexes III and IV.
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Cited by
Respiratory metabolism: glycolysis, the TCA cycle and mitochondrial electron transport
Fernie, A. R., Carrari, F., & Sweetlove, L. J. (2004) · Current Opinion in Plant Biology · doi:10.1016/j.pbi.2004.03.007 · Cited by 1,403
Foyer, C. H., & Noctor, G. (2003) · Physiologia Plantarum · doi:10.1034/j.1399-3054.2003.00223.x · Cited by 1,316
Self-citationDowdle, J., Ishikawa, T., Gatzek, S., Rolinski, S., & Smirnoff, N. (2007) · The Plant Journal · doi:10.1111/j.1365-313x.2007.03266.x · Cited by 459
Shalata, A., Mittova, V., Volokita, M., Guy, M., & Tal, M. (2001) · Physiologia Plantarum · doi:10.1034/j.1399-3054.2001.1120405.x · Cited by 448
Tomaz, T., Bagard, M., Pracharoenwattana, I., Lindén, P., Lee, C. P., Carroll, A. J., Ströher, E., Smith, S. M., Gardeström, P., & Millar, A. H. (2010) · Plant Physiology · doi:10.1104/pp.110.161612 · Cited by 264
Metadata
- Authors
- Bartoli, C. G., Pastori, G. M., & Foyer, C. H.
- Venue
- Plant Physiology
- Publisher
- Oxford University Press (OUP)
- Published
- 2000-05-01
- Volume
- 123
- Issue
- 1
- Pages
- 335-344
- ISSN
- 0032-0889 (ISSN-L)
- 1532-2548
- OpenAlex
- W2128319845
- Cited by
- 387
- References
- 0
- FWCI
- 11.35
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Bartoli, C. G., Pastori, G. M., & Foyer, C. H. (2000). Ascorbate Biosynthesis in Mitochondria Is Linked to the Electron Transport Chain between Complexes III and IV. Plant Physiology. https://doi.org/10.1104/pp.123.1.335
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