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Biochemical and Genetic Analysis of the Effects of Amylose-Extender Mutation in Rice Endosperm

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Akio Nishi·2001·PLANT PHYSIOLOGY
DOI 10.1104/pp.127.2.459
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Abstract

Biochemical analysis of amylose-extender (ae) mutant of rice (Oryza sativa) revealed that the mutation in the gene for starch-branching enzyme IIb (BEIIb) specifically altered the structure of amylopectin in the endosperm by reducing short chains with degree of polymerization of 17 or less, with the greatest decrease in chains with degree of polymerization of 8 to 12. The extent of such change was correlated with the gelatinization properties of the starch granules, as determined in terms of solubility in urea solution. The ae mutation caused a dramatic reduction in the activity of BEIIb. The activity of soluble starch synthase I (SSI) in the ae mutant was significantly lower than in the wild type, suggesting that the mutation had a pleiotropic effect on the SSI activity. In contrast, the activities of BEI, BEIIa, ADP-Glc pyrophosphorylase, isoamylase, isoamylase, pullulanase, and Suc synthase were not affected by the mutation. Therefore, it is stressed that the function of BEIIb cannot be complemented by BEIIa and BEI. These results strongly suggest that BEIIb plays a specific role in the transfer of short chains, which might then be extended by SS to form the A and B(1) chains of amylopectin cluster in rice endosperm.

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Metadata

Authors
Nishi, A.
Venue
PLANT PHYSIOLOGY
Publisher
Oxford University Press (OUP)
Published
2001-10-01
Volume
127
Issue
2
Pages
459-472
ISSN
  • 0032-0889 (ISSN-L)
  • 1532-2548
DOI
10.1104/pp.127.2.459
OpenAlex
W4255030633
Cited by
520
References
1
FWCI
8.55
Type
journal article
Language
en
Open access
green
Licence
academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model (vor)

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Nishi, A. (2001). Biochemical and Genetic Analysis of the Effects of Amylose-Extender Mutation in Rice Endosperm. PLANT PHYSIOLOGY. https://doi.org/10.1104/pp.127.2.459

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