The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Extinction Coefficients of Chlorophyll a and b in N,N-Dimethylformamide and 80% Acetone
Open accessAbstract
We found inconsistencies in the commonly used data for chlorophyll analysis in 80% acetone. Recently developed extinction coefficients for chlorophyll b in N,N-dimethylformamide (DMF) based on values from 80% acetone are low as a result of these inconsistencies. We determined extinction coefficients of chlorophyll a (Chl a) and chlorophyll b (Chl b) in DMF for wavelengths of 618 to 665 nanometers. The simultaneous equations necessary for quantifying Chl a, Chl b, or total Chl in DMF in the absence of other chlorophyllous pigments are: Chl a = 12.70A(664.5) - 2.79A(647); Chl b = 20.70 A(647) - 4.62A(664.5); total Chl = 17.90A(647) + 8.08A(664.5), where A = absorbance in 1.00 centimeter cuvettes and Chl = milligrams per liter.N,N-Dimethylformamide is a very convenient solvent for Chl extraction since it is effective on intact plant parts and Chl is quite stable in DMF. There was no difference in the amount of Chl extracted when plant tissue was stored for 1 or 3 days at three temperatures, with or without solvent added.
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Cites
Spectral absorption properties of ordinary and fully deuteriated chlorophylls a and b
Strain, H. H., Thomas, M. R., & Katz, J. J. (1963) · Biochimica et Biophysica Acta · doi:10.1016/0006-3002(63)90617-6 · Cited by 153
Cited by
Porra, R. J. (2002) · Photosynthesis Research · doi:10.1023/a:1020470224740 · Cited by 1,371
Quan, R., Lin, H., Mendoza, I., Zhang, Y., Cao, W., Yang, Y., Shang, M., Chen, S., Pardo, J. M., & Guo, Y. (2007) · The Plant Cell · doi:10.1105/tpc.106.042291 · Cited by 593
In Posidonia oceanica cadmium induces changes in DNA methylation and chromatin patterning
Greco, M., Chiappetta, A., Bruno, L., & Bitonti, M. B. (2012) · Journal of Experimental Botany · doi:10.1093/jxb/err313 · Cited by 538
PHYTOCHROME-INTERACTING FACTOR 1 Is a Critical bHLH Regulator of Chlorophyll Biosynthesis
Huq, E., Al-Sady, B., Hudson, M., Kim, C., Apel, K., & Quail, P. H. (2004) · Science · doi:10.1126/science.1099728 · Cited by 502
Photosynthetic Fractionation of the Stable Isotopes of Oxygen and Carbon
Guy, R. D., Fogel, M. L., & Berry, J. A. (1993) · Plant Physiology · doi:10.1104/pp.101.1.37 · Cited by 472
Metadata
- Authors
- Inskeep, W. P., & Bloom, P. R.
- Venue
- Plant Physiology
- Publisher
- Oxford University Press (OUP)
- Published
- 1985-02-01
- Volume
- 77
- Issue
- 2
- Pages
- 483-485
- ISSN
- 0032-0889 (ISSN-L)
- 1532-2548
- OpenAlex
- W2012850835
- Cited by
- 1,400
- References
- 1
- FWCI
- 3.50
- Type
- journal article
- Language
- en
- Open access
- bronze
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