Changes after Decapitation in Concentrations of Indole-3-Acetic Acid and Abscisic Acid in the Larger Axillary Bud of Phaseolus vulgaris L. cv Tender Green
Open accessAbstract
Early changes in the concentrations of indole-3-acetic acid (IAA) and abscisic acid (ABA) were investigated in the larger axillary bud of 2-week-old Phaseolus vulgaris L. cv Tender Green seedlings after removal of the dominant apical bud. Concentrations of these two hormones were measured at 4, 6, 8, 12 and 24 hours following decapitation of the apical bud and its subtending shoot. Quantitations were accomplished using either gas chromatography-mass spectrometry-selected ion monitoring (GS-MS-SIM) with [(13)C(6)]-IAA or [(2)H(6)]-ABA as quantitative internal standards, or by an indirect enzyme-linked immunosorbent assay, validated by GC-MS-SIM. Within 4 hours after decapitation the IAA concentration in the axillary bud had increased fivefold, remaining relatively constant thereafter. The concentration of ABA in axillary buds of decapitated plants was 30 to 70% lower than for buds of intact plants from 4 to 24 hours following decapitation. Fresh weight of buds on decapitated plants had increased by 8 hours after decapitation and this increase was even more prominent by 24 hours. Anatomical assessment of the larger axillary buds at 0, 8, and 24 hours following decapitation showed that most of the growth was due to cell expansion, especially in the intermodal region. Thus, IAA concentration in the axillary bud increases appreciably within a very few hours of decapitation. Coincidental with the rise in IAA concentration is a modest, but significant reduction in ABA concentration in these axillary buds after decapitation.
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Once growing, buds synthesize auxin.
“Once growing, buds synthesize auxin (Gocal et al., 1991) and its export may enhance vascular connections and nutrient flow to further stimulate the growing bud.”
From Strigolactone Acts Downstream of Auxin to Regulate Bud Outgrowth in Pea and Arabidopsis (2009) · cites this paper as Gocal et al., 1991
The Discussion (page not provided) reports that IAA concentration rose in axillary buds after decapitation, alongside bud growth. It does not establish that the growing buds synthesized the auxin; the study measured concentration, not its source. (LLM, conf=0.85)
Experimental — accuracy not yet measuredAuxin levels in the bud increase as lateral buds begin to enlarge and grow.
“auxin levels in the bud actually increase as lateral buds begin to enlarge and grow (Gocal et al., 1991;Hillman, 1984;Pearce et al., 1995).”
From The hormonal regulation of axillary bud growth in <i>Arabidopsis</i> (2000) · cites this paper as Gocal et al., 1991
Auxin levels increase as buds begin to grow, and bud activity correlates with the presence of active polar auxin transport.
“Certainly, auxin levels increase as buds begin to grow (Gocal et al., 1991;Hillman, 1984;Pearce et al., 1995), and bud activity correlates with the presence of active polar auxin transport (Morris and Johnson, 1990;Morris, 1977).”
From The hormonal regulation of axillary bud growth in <i>Arabidopsis</i> (2000) · cites this paper as Gocal et al., 1991
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References 17 · Cited by 120
Research works printed but not linked: 1
- Russell W, Thimann KV (1990) The second messenger in apical dominance controlled by auxin. In RP Pharis, SB Rood, eds, Plant Growth Substances 1988. Springer-Verlag, Heidelberg, pp 419-427
- Authors
- Gocal, G. F. W., Pharis, R. P., Yeung, E. C., & Pearce, D.
- Affiliations
- University of Calgary (CA)
- Venue
- Plant Physiology
- Publisher
- Oxford University Press (OUP)
- Published
- 1991-02-01
- Volume
- 95
- Issue
- 2
- Pages
- 344-350
- ISSN
- 0032-0889 (ISSN-L)
- 1532-2548
- PMC
- PMC1077536
- OpenAlex
- W1974529616
- Cited by
- 120
- References
- 17
- Citations by year
- 2026: 1 · 2025: 2 · 2024: 5 · 2023: 3 · 2022: 6 · 2021: 4 · 2020: 3 · 2019: 2 · 2018: 3 · 2017: 2 · 2016: 2 · 2015: 3 · 2014: 6 · 2013: 3 · 2012: 6
- FWCI
- 4.36
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- PLANT PHYSIOLOGY (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Molecular Biology Research
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Parasitism and Resistance
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Flowering Plant Growth and Cultivation
- Keywords
- indole-3-acetic acid, abscisic acid, axillary buds, decapitation, apical dominance, Phaseolus vulgaris, auxin, plant hormones, cell expansion, shoot growth
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