On the Role of Mitochondrial Oxidative Phosphorylation in Photosynthesis Metabolism as Studied by the Effect of Oligomycin on Photosynthesis in Protoplasts and Leaves of Barley (Hordeum vulgare)
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Low concentrations of oligomycin, which strongly inhibit mitochondrial oxidative phosphorylation but do not affect chloroplast photophosphorylation, caused an inhibition of photosynthesis by 30 to 40% in barley (Hordeum vulgare L.) leaf protoplasts. This inhibition is reversed and the full rate of photosynthesis is regained when the protoplasts are ruptured so as to leave the chloroplasts intact. Oligomycin fed into barley leaves by the transpiration stream inhibited photosynthesis in these leaves by up to 60%. The measurement of metabolites in protoplast and leaf extracts showed that oligomycin caused a decrease in the ATP/ADP ratio and an increase in the content of glucose- and fructose 6-phosphate. Subcellular analysis of protoplasts revealed that the decrease in ATP/ADP ratio in the cytosol was larger than in the stroma and that the increase in hexose monophosphates was restricted to the cytosol, whereas the stromal hexosemonophosphates decreased upon the addition of oligomycin. Moreover, oligomycin caused an increase in the triosephosphate-3-phosphoglycerate ratio. It is concluded from these results that during photosynthesis of a plant leaf cell mitochondrial oxidative phosphorylation contributes to the ATP supply of the cell and prevents overreduction of the chloroplast redox carriers by oxidizing reductive equivalents generated by photosynthetic electron transport.
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Addition of oligomycin to barley protoplasts decreased the ATP-ADP ratio in both mitochondria and cytosol, whereas the chloroplast ATP-ADP ratio was unchanged.
“the addition of oligomycin to barley (Hordeum vulgare) protoplasts decreased the ATP-ADP ratio in both mitochondria and cytosol, whereas the chloroplast ATP-ADP ratio was unchanged (Gardeström and Wigge, 1988; Krömer and Heldt, 1991; Wigge et al., 1993).”
From Leaf Energy Balance Requires Mitochondrial Respiration and Export of Chloroplast NADPH in the Light (2019) · cites this paper as Krömer and Heldt, 1991
Table II (Effect of Oligomycin on Metabolite Levels in Barley Protoplasts) shows that the stromal ATP/ADP ratio fell from 0.99 to 0.60 after oligomycin, contrary to the claim that the chloroplast ratio was unchanged. It also reports cytosol and mitochondria together, rather than separate ratios for each. (LLM, conf=0.90)
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Research works printed but not linked: 4
- Ebbighausen H (1986) Zur Funktion der Pflanzenmitochondrien bei der Photorespiration. Dissertation zur Erlangung des Dok- torgrades, Universitdt Gottingen, FRG
- Heldt HW, Heineke D, Heupel R, Kromer S, Riens B (1990) Transfer of redox equivalents between subcellular compart- ments of a leaf cell. In M Baltscheffsky, ed, Proceedings of the VIIIth International Conference on Photosynthesis, Sweden, Academic Press, Dodrecht, IV: 15.1-15.
- Pier PA, Berkowitz GA (1987) Modulation ofwater stress effects Plant Physiol. Vol. 95, 1991 on photosynthesis by altered leaf K+. Plant Physiol 85: 655- 661
- Slater EC, Ter Welle HF (1969) Applications of oligomycin and related inhibitors in bioenergetics. In T Bucher, H Sies, eds, Inhibitors, Tools in Cell Research. Springer-Verlag; Berlin, pp 258-278
Cited by
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Leaf Energy Balance Requires Mitochondrial Respiration and Export of Chloroplast NADPH in the Light
Shameer, S., Ratcliffe, R. G., & Sweetlove, L. J. (2019) · Plant Physiology · doi:10.1104/pp.19.00624 · Cited by 118 · see citing works
NAD <sup>+</sup> Biosynthesis and Signaling in Plants
Gakière, B., Hao, J., de Bont, L., Pétriacq, P., Nunes-Nesi, A., & Fernie, A. R. (2018) · Critical Reviews in Plant Sciences · doi:10.1080/07352689.2018.1505591 · Cited by 108 · see citing works
The origin of cytosolic <scp>ATP</scp> in photosynthetic cells
Gardeström, P., & Igamberdiev, A. U. (2016) · Physiologia Plantarum · doi:10.1111/ppl.12455 · Cited by 94 · see citing works
Wigge, B., Krömer, S., & Gardeström, P. (1993) · Physiologia Plantarum · doi:10.1111/j.1399-3054.1993.tb01754.x · Cited by 87 · see citing works
Self-citation
- Authors
- Krömer, S., & Heldt, H. W.
- Affiliations
- University of Göttingen (DE)
- Venue
- Plant Physiology
- Publisher
- Oxford University Press (OUP)
- Published
- 1991-04-01
- Volume
- 95
- Issue
- 4
- Pages
- 1270-1276
- ISSN
- 0032-0889 (ISSN-L)
- 1532-2548
- PMC
- PMC1077684
- OpenAlex
- W1998674709
- Cited by
- 124
- References
- 0
- Citations by year
- 2026: 1 · 2024: 2 · 2023: 3 · 2022: 2 · 2021: 2 · 2019: 1 · 2018: 7 · 2017: 3 · 2016: 5 · 2015: 2 · 2014: 1 · 2012: 1
- FWCI
- 3.57
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- PLANT PHYSIOLOGY (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Photosynthetic Processes and Mechanisms
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant nutrient uptake and metabolism
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Mitochondrial Function and Pathology
- Keywords
- mitochondrial oxidative phosphorylation, photosynthesis, oligomycin, barley, protoplasts, ATP/ADP ratio, hexose monophosphates, photosynthetic electron transport, transpiration stream, chloroplast stroma, cytosol
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