The origin of cytosolic ATP in photosynthetic cells
Abstract
In photosynthetically active cells, both chloroplasts and mitochondria have the capacity to produce ATP via photophosphorylation and oxidative phosphorylation, respectively. Thus, theoretically, both organelles could provide ATP for the cytosol, but the extent, to which they actually do this, and how the process is regulated, both remain unclear. Most of the evidence discussed comes from experiments with rapid fractionation of isolated protoplasts subjected to different treatments in combination with application of specific inhibitors. The results obtained indicate that, under conditions where ATP demand for photosynthetic CO 2 fixation is sufficiently high, the mitochondria supply the bulk of ATP for the cytosol. In contrast, under stress conditions where CO 2 fixation is severely limited, ATP will build up in chloroplasts and it can then be exported to the cytosol, by metabolite shuttle mechanisms. Thus, depending on the conditions, either mitochondria or chloroplasts can supply the bulk of ATP for the cytosol. This supply of ATP is discussed in relation to the idea that mitochondrial functions may be tuned to provide an optimal environment for the chloroplast. By balancing cellular redox states, mitochondria can contribute to an optimal photosynthetic capacity.
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A proportion of the NADH from Gly oxidation is oxidized via the mitochondrial respiratory chain to generate ATP by oxidative phosphorylation.
“The use of inhibitors of the mitochondrial ATP synthase and Gly decarboxylase suggests that a proportion of the NADH from Gly oxidation is oxidized via the mitochondrial respiratory chain to generate ATP by oxidative phosphorylation (Gardeström and Igamberdiev, 2016).”
From Leaf Energy Balance Requires Mitochondrial Respiration and Export of Chloroplast NADPH in the Light (2019) · cites this paper as Gardeström and Igamberdiev, 2016
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- Authors
- Gardeström, P., & Igamberdiev, A. U.
- Affiliations
- Umeå Plant Science Centre (SE)
- Memorial University of Newfoundland (CA)
- Corresponding author
- Gardeström, Per
- Venue
- Physiologia Plantarum
- Publisher
- Wiley
- Published
- 2016-05-26
- Volume
- 157
- Issue
- 3
- Pages
- 367-379
- ISSN
- 0031-9317 (ISSN-L)
- 1399-3054
- OpenAlex
- W2340884884
- Cited by
- 94
- References
- 75
- Citations by year
- 2026: 5 · 2025: 7 · 2024: 11 · 2023: 8 · 2022: 6 · 2021: 14 · 2020: 14 · 2019: 9 · 2018: 9 · 2017: 6 · 2016: 5
- FWCI
- 0.87
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Physiologia Plantarum (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Vetenskapsrådet
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Photosynthetic Processes and Mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Mitochondrial Function and Pathology
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant nutrient uptake and metabolism
- Keywords
- cytosolic ATP, ATP supply, chloroplasts, mitochondria, photophosphorylation, oxidative phosphorylation, photosynthetic carbon fixation, redox state, photosynthetic stress, chloroplast-mitochondria interaction
- MeSH terms
- Adenosine Triphosphate, Carbon Dioxide, Chloroplasts, Cytosol, Light, Mitochondria, Oxidation-Reduction, Photosynthesis, Plants, Protoplasts, Organelles
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