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Cited by 228

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Uptake of a Fluorescent Marker in Plant Cells Is Sensitive to Brefeldin A and Wortmannin

Open access
Emans, Neil, Zimmermann, Sabine, Fischer, Rainer·2002·The Plant Cell

Abstract

We assessed FM1-43 [N-(3-triethylammoniumpropyl)-4-(4-[dibutylamino]styryl)pyridinium dibromide] as a fluorescent endocytosis marker in intact, walled plant cells. At 4 degrees C, FM1-43 stained the plasma membrane, and after 30 to 120 min of incubation at 26 degrees C, FM1-43 labeled cytoplasmic vesicles and then the vacuole. Fluorimetric quantitation demonstrated dye uptake temperature sensitivity (approximately 65% reduction at 16 degrees C, >90% at 4 degrees C). FM1-43 uptake in suspension cells was stimulated more than twofold by brefeldin A and inhibited approximately 0.4-fold by wortmannin. FM1-43 delivery to the vacuole was largely inhibited by brefeldin A, although overall uptake was stimulated, and brefeldin A treatment caused the accumulation of large prevacuolar endosomal vesicles heavily labeled with FM1-43. Three-dimensional time lapse imaging revealed that FM1-43-labeled vacuoles and vesicles are highly dynamic. Thus, FM1-43 serves as a fluorescent marker for imaging and quantifying membrane endocytosis in intact plant cells.

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Crossref · 10.1105/tpc.010339

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Metadata

Authors
Emans, N., Zimmermann, S., & Fischer, R.
Venue
The Plant Cell
Published
2002-01
Volume
14
Issue
1
Pages
71-86
ISSN
  • 1040-4651 (ISSN-L)
  • 1532-298X
OpenAlex
W2098918259
Cited by
228
References
2
FWCI
5.48
Type
journal article
Language
en
Open access
bronze

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