The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
The DWF4 Gene of Arabidopsis Encodes a Cytochrome P450 That Mediates Multiple 22α-Hydroxylation Steps in Brassinosteroid Biosynthesis
Open accessAbstract
dwarf4 (dwf4) mutants of Arabidopsis display a dwarfed phenotype due to a lack of cell elongation. Dwarfism could be rescued by the application of brassinolide, suggesting that DWF4 plays a role in brassinosteroid (BR) biosynthesis. The DWF4 locus is defined by four mutant alleles. One of these is the result of a T-DNA insertion. Plant DNA flanking the insertion site was cloned and used as a probe to isolate the entire DWF4 gene. Sequence analysis revealed that DWF4 encodes a cytochrome P450 monooxygenase with 43% identity to the putative Arabidopsis steroid hydroxylating enzyme CONSTITUTIVE PHOTOMORPHOGENESIS AND DWARFISM. Sequence analysis of two other mutant alleles revealed deletions or a premature stop codon, confirming that DWF4 had been cloned. This sequence similarity suggests that DWF4 functions in specific hydroxylation steps during BR biosynthesis. In fact, feeding studies utilizing BR intermediates showed that only 22alpha-hydroxylated BRs rescued the dwf4 phenotype, confirming that DWF4 acts as a 22alpha-hydroxylase.
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Citations
Cites
Identification of the Retinoic Acid-inducible All-trans-retinoic Acid 4-Hydroxylase
White, J. A., Guo, Y.-D., Baetz, K., Beckett-Jones, B., Bonasoro, J., Hsu, K. E., Dilworth, F. J., Jones, G., & Petkovich, M. (1996) · Journal of Biological Chemistry · doi:10.1074/jbc.271.47.29922 · Cited by 382
Cited by
Li, J., Wen, J., Lease, K. A., Doke, J. T., Tax, F. E., & Walker, J. C. (2002) · Cell · doi:10.1016/s0092-8674(02)00812-7 · Cited by 1,446
BRI1 is a critical component of a plasma-membrane receptor for plant steroids
Wang, Z.-Y., Seto, H., Fujioka, S., Yoshida, S., & Chory, J. (2001) · Nature · doi:10.1038/35066597 · Cited by 846
Self-citationSakamoto, T., Morinaka, Y., Ohnishi, T., Sunohara, H., Fujioka, S., Ueguchi-Tanaka, M., Mizutani, M., Sakata, K., Takatsuto, S., Yoshida, S., et al. (2006) · Nature Biotechnology · doi:10.1038/nbt1173 · Cited by 745
Self-citationTanabe, S., Ashikari, M., Fujioka, S., Takatsuto, S., Yoshida, S., Yano, M., Yoshimura, A., Kitano, H., Matsuoka, M., Fujisawa, Y., et al. (2005) · The Plant Cell · doi:10.1105/tpc.104.024950 · Cited by 555
Self-citationThe Arabidopsis DELAYED DEHISCENCE1 Gene Encodes an Enzyme in the Jasmonic Acid Synthesis Pathway
Sanders, P. M., Lee, P. Y., Biesgen, C., Boone, J. D., Beals, T. P., Weiler, E. W., & Goldberg, R. B. (2000) · The Plant Cell · doi:10.1105/tpc.12.7.1041 · Cited by 526
Metadata
- Authors
- Choe, S., Dilkes, B. P., Fujioka, S., Takatsuto, S., Sakurai, A., & Feldmann, K. A.
- Venue
- The Plant Cell
- Publisher
- Oxford University Press (OUP)
- Published
- 1998-02
- Volume
- 10
- Issue
- 2
- Pages
- 231-243
- ISSN
- 1040-4651 (ISSN-L)
- 1532-298X
- OpenAlex
- W2148121686
- Cited by
- 483
- References
- 1
- FWCI
- 8.10
- Type
- journal article
- Language
- en
- Open access
- green
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