BRI1 is a critical component of a plasma-membrane receptor for plant steroids
Abstract
Most multicellular organisms use steroids as signalling molecules for physiological and developmental regulation. Two different modes of steroid action have been described in animal systems: the well-studied gene regulation response mediated by nuclear receptors, and the rapid non-genomic responses mediated by proposed membrane-bound receptors. Plant genomes do not seem to encode members of the nuclear receptor superfamily. However, a transmembrane receptor kinase, brassinosteroid-insensitive1 (BRI1), has been implicated in brassinosteroid responses. Here we show that BRI1 functions as a receptor of brassinolide, the most active brassinosteroid. The number of brassinolide-binding sites and the degree of response to brassinolide depend on the level of BRI1 protein. The brassinolide-binding activity co-immunoprecipitates with BRI1, and requires a functional BRI1 extracellular domain. Moreover, treatment of Arabidopsis seedlings with brassinolide induces autophosphorylation of BRI1, which, together with our binding studies, shows that BRI1 is a receptor kinase that transduces steroid signals across the plasma membrane.
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Animal steroid hormones act through the nuclear receptor family of transcription factors, while plant brassinosteroids signal through a cell surface receptor kinase-mediated signal transduction pathway.
“While animal steroid hormones act through nuclear receptor family of transcription factors, plant brassinosteroids signal through a cell surface receptor kinase-mediated signal transduction pathway (8, 9).”
From Anabolic effect of plant brassinosteroid (2011) · cites this paper as [8]
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- Authors
- Wang, Z.-Y., Seto, H., Fujioka, S., Yoshida, S., & Chory, J.
- Affiliations
- RIKEN (JP)
- Salk Institute for Biological Studies (US)
- Corresponding author
- Chory, Joanne
- Venue
- Nature
- Published
- 2001-03-15
- Volume
- 410
- Issue
- 6826
- Pages
- 380-383
- ISSN
- 0028-0836 (ISSN-L)
- 1476-4687
- OpenAlex
- W1994207647
- Cited by
- 846
- References
- 25
- Citations by year
- 2026: 17 · 2025: 31 · 2024: 33 · 2023: 32 · 2022: 40 · 2021: 30 · 2020: 30 · 2019: 25 · 2018: 30 · 2017: 44 · 2016: 29 · 2015: 26 · 2014: 27 · 2013: 40 · 2012: 26
- FWCI
- 66.86
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Nature (publishedVersion)
- PubMed (publishedVersion)
- RePEc: Research Papers in Economics (submittedVersion)
- Funders
- National Science Foundation
- Howard Hughes Medical Institute
- U.S. Department of Agriculture
- National Alliance for Research on Schizophrenia and Depression
- Topics
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Molecular Biology Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Plant biochemistry and biosynthesis
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Plant Reproductive Biology
- Keywords
- BRI1, brassinosteroid signaling, brassinolide, plasma membrane receptors, receptor kinases, autophosphorylation, Arabidopsis thaliana
- MeSH terms
- Cholestanols, Enzyme Activation, Ligands, Mutation, Phosphorylation, Phytosterols, Protein Kinases, Receptors, Steroid, Steroids, Heterocyclic, Signal Transduction, Genes, Plant, Arabidopsis, Protein Structure, Tertiary, Arabidopsis Proteins, Brassinosteroids
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