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Barley Aleurone Cells Contain Two Types of Vacuoles: Characterization of Lytic Organelles by Use of Fluorescent Probes
Open accessAbstract
Light microscopy was used to study the structure and function of vacuoles in living protoplasts of barley (Hordeum vulgare cv Himalaya) aleurone. Light microscopy showed that aleurone protoplasts contain two distinct types of vacuole: the protein storage vacuole and a lysosome-like organelle, which we have called the secondary vacuole. Fluorescence microscopy using pH-sensitive fluorescent probes and a fluorogenic substrate for cysteine proteases showed that both protein storage vacuoles and secondary vacuoles are acidic, lytic organelles. Ratio imaging showed that the pH of secondary vacuoles was lower in aleurone protoplasts incubated in gibberellic acid than in those incubated in abscisic acid. Uptake of fluorescent probes into intact, isolated protein storage vacuoles and secondary vacuoles required ATP and occurred via at least two types of vanadate-sensitive, ATP-dependent tonoplast transporters. One transporter catalyzed the accumulation of glutathione-conjugated probes, and another transported probes not conjugated to glutathione.
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Cited by
Yoshimoto, K., Hanaoka, H., Sato, S., Kato, T., Tabata, S., Noda, T., & Ohsumi, Y. (2004) · The Plant Cell · doi:10.1105/tpc.104.025395 · Cited by 603
Bassil, E., Tajima, H., Liang, Y.-C., Ohto, M.-A., Ushijima, K., Nakano, R., Esumi, T., Coku, A., Belmonte, M., & Blumwald, E. (2011) · The Plant Cell · doi:10.1105/tpc.111.089581 · Cited by 470
Plant proteases, protein degradation, and oxidative stress: role of peroxisomes
Palma, J. M., Sandalio, L. M., Javier Corpas, F., Romero-Puertas, M. C., McCarthy, I., & del Río, L. A. (2002) · Plant Physiology and Biochemistry · doi:10.1016/s0981-9428(02)01404-3 · Cited by 447
E NDOSPERM D EVELOPMENT: Cellularization and Cell Fate Specification
Olsen, O.-A. (2001) · Annual Review of Plant Physiology and Plant Molecular Biology · doi:10.1146/annurev.arplant.52.1.233 · Cited by 384
T ONOPLAST T RANSPORTERS: Organization and Function
Maeshima, M. (2001) · Annual Review of Plant Physiology and Plant Molecular Biology · doi:10.1146/annurev.arplant.52.1.469 · Cited by 287
Metadata
- Authors
- Swanson, S. J., Bethke, P. C., & Jones, R. L.
- Venue
- The Plant Cell
- Publisher
- Oxford University Press (OUP)
- Published
- 1998-05
- Volume
- 10
- Issue
- 5
- Pages
- 685-698
- ISSN
- 1040-4651 (ISSN-L)
- 1532-298X
- OpenAlex
- W1972321507
- Cited by
- 173
- References
- 0
- FWCI
- 6.20
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Swanson, S. J., Bethke, P. C., & Jones, R. L. (1998). Barley Aleurone Cells Contain Two Types of Vacuoles: Characterization of Lytic Organelles by Use of Fluorescent Probes. The Plant Cell. https://doi.org/10.1105/tpc.10.5.685
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