The DOI matches its Crossref record; 52 of the 52 references it cites were found in a registry or an index.
Lotus japonicus CASTOR and POLLUX Are Ion Channels Essential for Perinuclear Calcium Spiking in Legume Root Endosymbiosis
Open accessAbstract
The mechanism underlying perinuclear calcium spiking induced during legume root endosymbioses is largely unknown. Lotus japonicus symbiosis-defective castor and pollux mutants are impaired in perinuclear calcium spiking. Homology modeling suggested that the related proteins CASTOR and POLLUX might be ion channels. Here, we show that CASTOR and POLLUX form two independent homocomplexes in planta. CASTOR reconstituted in planar lipid bilayers exhibited ion channel activity, and the channel characteristics were altered in a symbiosis-defective mutant carrying an amino acid replacement close to the selectivity filter. Permeability ratio determination and competition experiments reveled a weak preference of CASTOR for cations such as potassium over anions. POLLUX has an identical selectivity filter region and complemented a potassium transport–deficient yeast mutant, suggesting that POLLUX is also a potassium-permeable channel. Immunogold labeling localized the endogenous CASTOR protein to the nuclear envelope of Lotus root cells. Our data are consistent with a role of CASTOR and POLLUX in modulating the nuclear envelope membrane potential. They could either trigger the opening of calcium release channels or compensate the charge release during the calcium efflux as counter ion channels.
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The Language of Calcium Signaling
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Invasion by Invitation: Rhizobial Infection in Legumes
Murray, J. D. (2011) · Molecular Plant-Microbe Interactions® · doi:10.1094/mpmi-08-10-0181 · Cited by 245
Roberts, N. J., Morieri, G., Kalsi, G., Rose, A., Stiller, J., Edwards, A., Xie, F., Gresshoff, P. M., Oldroyd, G. E. D., Downie, J. A., et al. (2012) · Plant Physiology · doi:10.1104/pp.112.206110 · Cited by 49
Metadata
- Authors
- Charpentier, M., Bredemeier, R., Wanner, G., Takeda, N., Schleiff, E., & Parniske, M.
- Venue
- The Plant Cell
- Publisher
- Oxford University Press (OUP)
- Published
- 2008-12-01
- Volume
- 20
- Issue
- 12
- Pages
- 3467-3479
- ISSN
- 1040-4651 (ISSN-L)
- 1532-298X
- OpenAlex
- W2168428619
- Cited by
- 257
- References
- 52
- FWCI
- 5.58
- Type
- journal article
- Language
- en
- Open access
- green
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- CoChat. (2026). Citation check: Lotus japonicus CASTOR and POLLUX Are Ion Channels Essential for Perinuclear Calcium Spiking in Legume Root Endosymbiosis. https://app.cochat.ai/papers/10.1105/tpc.108.063255
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- Charpentier, M., Bredemeier, R., Wanner, G., Takeda, N., Schleiff, E., & Parniske, M. (2009). Lotus japonicus CASTOR and POLLUX Are Ion Channels Essential for Perinuclear Calcium Spiking in Legume Root Endosymbiosis. The Plant Cell. https://doi.org/10.1105/tpc.108.063255
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