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A Chloroplastic UDP-Glucose Pyrophosphorylase from Arabidopsis Is the Committed Enzyme for the First Step of Sulfolipid Biosynthesis
Open accessAbstract
Plants synthesize a sulfur-containing lipid, sulfoquinovosyldiacylglycerol, which is one of three nonphosphorus glycerolipids that provide the bulk of the structural lipids in photosynthetic membranes. Here, the identification of a novel gene, UDP-glucose pyrophosphorylase3 (UGP3), required for sulfolipid biosynthesis is described. Transcriptome coexpression analysis demonstrated highly correlated expression of UGP3 with known genes for sulfolipid biosynthesis in Arabidopsis thaliana. Liquid chromatography–mass spectrometry analysis of leaf lipids in two Arabidopsis ugp3 mutants revealed that no sulfolipid was accumulated in these mutants, indicating the participation of UGP3 in sulfolipid biosynthesis. From the deduced amino acid sequence, UGP3 was presumed to be a UDP-glucose pyrophosphorylase (UGPase) involved in the generation of UDP-glucose, serving as the precursor of the polar head of sulfolipid. Recombinant UGP3 was able to catalyze the formation of UDP-glucose from glucose-1-phosphate and UTP. A transient assay using fluorescence fusion proteins and UGPase activity in isolated chloroplasts indicated chloroplastic localization of UGP3. The transcription level of UGP3 was increased by phosphate starvation. A comparative genomics study on UGP3 homologs across different plant species suggested the structural and functional conservation of the proteins and, thus, a committing role for UGP3 in sulfolipid synthesis.
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A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION
Bligh, E. G., & Dyer, W. J. (1959) · Canadian Journal of Biochemistry and Physiology · doi:10.1139/o59-099 · Cited by 54,751
Decoding genes with coexpression networks and metabolomics – ‘majority report by precogs’
Saito, K., Hirai, M. Y., & Yonekura-Sakakibara, K. (2008) · Trends in Plant Science · doi:10.1016/j.tplants.2007.10.006 · Cited by 327
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Wang, Y., Tang, H., DeBarry, J. D., Tan, X., Li, J., Wang, X., Lee, T.-H., Jin, H., Marler, B., Guo, H., et al. (2012) · Nucleic Acids Research · doi:10.1093/nar/gkr1293 · Cited by 7,093
Takahashi, H., Kopriva, S., Giordano, M., Saito, K., & Hell, R. (2011) · Annual Review of Plant Biology · doi:10.1146/annurev-arplant-042110-103921 · Cited by 893
Self-citationPGDD: a database of gene and genome duplication in plants
Lee, T.-H., Tang, H., Wang, X., & Paterson, A. H. (2012) · Nucleic Acids Research · doi:10.1093/nar/gks1104 · Cited by 593
Li-Beisson, Y., Shorrosh, B., Beisson, F., Andersson, M. X., Arondel, V., Bates, P. D., Baud, S., Bird, D., DeBono, A., Durrett, T. P., et al. (2010) · The Arabidopsis Book · doi:10.1199/tab.0133 · Cited by 367
Salem, M. A., Perez de Souza, L., Serag, A., Fernie, A. R., Farag, M. A., Ezzat, S. M., & Alseekh, S. (2020) · Metabolites · doi:10.3390/metabo10010037 · Cited by 361
Metadata
- Authors
- Okazaki, Y., Shimojima, M., Sawada, Y., Toyooka, K., Narisawa, T., Mochida, K., Tanaka, H., Matsuda, F., Hirai, A., Hirai, M. Y., Ohta, H., & Saito, K.
- Venue
- The Plant Cell
- Publisher
- Oxford University Press (OUP)
- Published
- 2009-03-01
- Volume
- 21
- Issue
- 3
- Pages
- 892-909
- ISSN
- 1040-4651 (ISSN-L)
- 1532-298X
- OpenAlex
- W2112058589
- Cited by
- 210
- References
- 2
- FWCI
- 8.66
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Okazaki, Y., Shimojima, M., Sawada, Y., Toyooka, K., Narisawa, T., Mochida, K., Tanaka, H., Matsuda, F., Hirai, A., Hirai, M. Y., Ohta, H., & Saito, K. (2009). A Chloroplastic UDP-Glucose Pyrophosphorylase from Arabidopsis Is the Committed Enzyme for the First Step of Sulfolipid Biosynthesis. The Plant Cell. https://doi.org/10.1105/tpc.108.063925
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