The DOI matches its Crossref record; 3 of the 3 references it cites were found in a registry or an index.
HIGS: Host-Induced Gene Silencing in the Obligate Biotrophic Fungal Pathogen Blumeria graminis
Open accessAbstract
Powdery mildew fungi are obligate biotrophic pathogens that only grow on living hosts and cause damage in thousands of plant species. Despite their agronomical importance, little direct functional evidence for genes of pathogenicity and virulence is currently available because mutagenesis and transformation protocols are lacking. Here, we show that the accumulation in barley (Hordeum vulgare) and wheat (Triticum aestivum) of double-stranded or antisense RNA targeting fungal transcripts affects the development of the powdery mildew fungus Blumeria graminis. Proof of concept for host-induced gene silencing was obtained by silencing the effector gene Avra10, which resulted in reduced fungal development in the absence, but not in the presence, of the matching resistance gene Mla10. The fungus could be rescued from the silencing of Avra10 by the transient expression of a synthetic gene that was resistant to RNA interference (RNAi) due to silent point mutations. The results suggest traffic of RNA molecules from host plants into B. graminis and may lead to an RNAi-based crop protection strategy against fungal pathogens.
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Cites
Valadi, H., Ekström, K., Bossios, A., Sjöstrand, M., Lee, J. J., & Lötvall, J. O. (2007) · Nature Cell Biology · doi:10.1038/ncb1596 · Cited by 12,834
Mao, Y.-B., Cai, W.-J., Wang, J.-W., Hong, G.-J., Tao, X.-Y., Wang, L.-J., Huang, Y.-P., & Chen, X.-Y. (2007) · Nature Biotechnology · doi:10.1038/nbt1352 · Cited by 1,358 · cited 1×
“Recently, reduced development of rootknot nematodes as well as Lepidoptera and Coleoptera insects feeding on transgenic plants that carry RNAi constructs against target genes in these pests has been reported (Huang et al., 2006; Baum et al., 2007; Mao et al., 2007).”
BackgroundShen, Q.-H., Saijo, Y., Mauch, S., Biskup, C., Bieri, S., Keller, B., Seki, H., Ülker, B., Somssich, I. E., & Schulze-Lefert, P. (2007) · Science · doi:10.1126/science.1136372 · Cited by 751
Cited by
Genetic Variation and Unintended Risk in the Context of Old and New Breeding Techniques
Singer, S. D., Laurie, J. D., Bilichak, A., Kumar, S., & Singh, J. (2021) · Critical Reviews in Plant Sciences · doi:10.1080/07352689.2021.1883826 · Cited by 77
Metadata
- Authors
- Nowara, D., Gay, A., Lacomme, C., Shaw, J., Ridout, C., Douchkov, D., Hensel, G., Kumlehn, J., & Schweizer, P.
- Venue
- The Plant Cell
- Publisher
- Oxford University Press (OUP)
- Published
- 2010-09-01
- Volume
- 22
- Issue
- 9
- Pages
- 3130-3141
- ISSN
- 1040-4651 (ISSN-L)
- 1532-298X
- OpenAlex
- W2104161044
- Cited by
- 750
- References
- 3
- FWCI
- 28.74
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Nowara, D., Gay, A., Lacomme, C., Shaw, J., Ridout, C., Douchkov, D., Hensel, G., Kumlehn, J., & Schweizer, P. (2010). HIGS: Host-Induced Gene Silencing in the Obligate Biotrophic Fungal Pathogen Blumeria graminis. The Plant Cell. https://doi.org/10.1105/tpc.110.077040
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