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Cited by 292Insect-Plant Interactions and ControlGenetic Mapping and Diversity in Plants and AnimalsInsect Resistance and Genetics

Natural Variation in Maize Aphid Resistance Is Associated with 2,4-Dihydroxy-7-Methoxy-1,4-Benzoxazin-3-One Glucoside Methyltransferase Activity

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Meihls, Lisa N., Handrick, Vinzenz, Glauser, Gaetan, Barbier, Hugues, Kaur, Harleen, Haribal, Meena M., Lipka, Alexander E., Gershenzon, Jonathan +4 more·2013·The Plant Cell
DOI 10.1105/tpc.113.112409PMC PMC3723630
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Abstract

Plants differ greatly in their susceptibility to insect herbivory, suggesting both local adaptation and resistance tradeoffs. We used maize (Zea mays) recombinant inbred lines to map a quantitative trait locus (QTL) for the maize leaf aphid (Rhopalosiphum maidis) susceptibility to maize Chromosome 1. Phytochemical analysis revealed that the same locus was also associated with high levels of 2-hydroxy-4,7-dimethoxy-1,4-benzoxazin-3-one glucoside (HDMBOA-Glc) and low levels of 2,4-dihydroxy-7-methoxy-1,4-benzoxazin-3-one glucoside (DIMBOA-Glc). In vitro enzyme assays with candidate genes from the region of the QTL identified three O-methyltransferases (Bx10a-c) that convert DIMBOA-Glc to HDMBOA-Glc. Variation in HDMBOA-Glc production was attributed to a natural CACTA family transposon insertion that inactivates Bx10c in maize lines with low HDMBOA-Glc accumulation. When tested with a population of 26 diverse maize inbred lines, R. maidis produced more progeny on those with high HDMBOA-Glc and low DIMBOA-Glc. Although HDMBOA-Glc was more toxic to R. maidis than DIMBOA-Glc in vitro, BX10c activity and the resulting decline of DIMBOA-Glc upon methylation to HDMBOA-Glc were associated with reduced callose deposition as an aphid defense response in vivo. Thus, a natural transposon insertion appears to mediate an ecologically relevant trade-off between the direct toxicity and defense-inducing properties of maize benzoxazinoids.

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  • Benzoxazinoid-deficient bx1 maize mutants are defective in aphid- and chitosan-induced callose deposition, and callose induction is rescued by addition of DIMBOA or DIMBOA-Glc.

    “Benzoxazinoid-deficient bx1 maize (Zea mays) mutants are defective in aphid-and chitosan-induced callose deposition, and callose induction is rescued by the addition of DIMBOA or DIMBOA-Glc (Ahmad et al., 2011;Meihls et al., 2013).”

    From Plant Secondary Metabolites as Defenses, Regulators, and Primary Metabolites: The Blurred Functional Trichotomy (2020) · cites this paper as Meihls et al., 2013

    The Introduction and Discussion (page numbers unavailable) report reduced callose accumulation in a bx1 igl1 double mutant and callose induction after DIMBOA infiltration. The Results also associate low DIMBOA-Glc with reduced aphid-induced callose. Our copy does not establish defective chitosan-induced callose deposition or rescue by adding DIMBOA-Glc, and its mutant finding concerns a double mutant rather than bx1 alone. (LLM, conf=0.88)

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Authors
Meihls, L. N., Handrick, V., Glauser, G., Barbier, H., Kaur, H., Haribal, M. M., Lipka, A. E., Gershenzon, J., Buckler, E. S., Erb, M., Köllner, T. G., & Jander, G.
Affiliations
  • Boyce Thompson Institute (US)
  • Max Planck Society (DE)
  • Institute of Biology, University of Neuchâtel, 2009 Neuchatel, Switzerland
  • Agricultural Research Service (US)
ORCID
Venue
The Plant Cell
Publisher
Oxford University Press (OUP)
Published
2013-06-01
Volume
25
Issue
6
Pages
2341-2355
ISSN
  • 1040-4651 (ISSN-L)
  • 1532-298X
DOI
10.1105/tpc.113.112409
PMC
PMC3723630
OpenAlex
W2134990135
Cited by
292
References
0
Citations by year
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FWCI
15.71
Type
journal article
Language
en
Open access
Licence
academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model (vor)
Locations
  • The Plant Cell (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
  • BORIS (University Library Bern) (submittedVersion)
  • Max Planck Digital Library (submittedVersion)
  • MPG.PuRe (Max Planck Society) (submittedVersion)
Topics
  • Life Sciences › Agricultural and Biological Sciences › Insect Science › Insect-Plant Interactions and Control
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Genetic Mapping and Diversity in Plants and Animals
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Insect Resistance and Genetics
Keywords
Rhopalosiphum maidis, benzoxazinoids, O-methyltransferase, quantitative trait loci, callose deposition, plant-insect interactions, defense trade-offs, recombinant inbred lines
MeSH terms
Amino Acid Sequence, Animals, Aphids, Chromosome Mapping, Zea mays, DNA Transposable Elements, Glucosides, Host-Parasite Interactions, Isoenzymes, Methyltransferases, Molecular Sequence Data, Phylogeny, Plant Diseases, Plant Proteins, Mutagenesis, Insertional, Sequence Homology, Amino Acid, Chromosomes, Plant, Quantitative Trait Loci, Benzoxazines, Disease Resistance

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