The DOI matches its Crossref record; 44 of the 44 references it cites were found in a registry or an index.
Downregulation of Caffeic Acid 3-O-Methyltransferase and Caffeoyl CoA 3-O-Methyltransferase in Transgenic Alfalfa: Impacts on Lignin Structure and Implications for the Biosynthesis of G and S Lignin
Open accessAbstract
Transgenic alfalfa plants were generated harboring caffeic acid 3-O-methyltransferase (COMT) and caffeoyl CoA 3-O-methyltransferase (CCOMT) cDNA sequences under control of the bean phenylalanine ammonia-lyase PAL2 promoter. Strong downregulation of COMT resulted in decreased lignin content, a reduction in total guaiacyl (G) lignin units, a near total loss of syringyl (S) units in monomeric and dimeric lignin degradation products, and appearance of low levels of 5-hydroxy guaiacyl units and a novel dimer. No soluble monolignol precursors accumulated. In contrast, strong downregulation of CCOMT led to reduced lignin levels, a reduction in G units without reduction in S units, and increases in beta-5 linked dimers of G units. Accumulation of soluble caffeic acid beta-d-glucoside occurred only in CCOMT downregulated plants. The results suggest that CCOMT does not significantly contribute to the 3-O-methylation step in S lignin biosynthesis in alfalfa and that there is redundancy with respect to the 3-O-methylation reaction of G lignin biosynthesis. COMT is unlikely to catalyze the in vivo methylation of caffeic acid during lignin biosynthesis.
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Structure and function of enzymes involved in the biosynthesis of phenylpropanoids
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Metadata
- Authors
- Guo, D., Chen, F., Inoue, K., Blount, J. W., & Dixon, R. A.
- Venue
- The Plant Cell
- Publisher
- Oxford University Press (OUP)
- Published
- 2001-01
- Volume
- 13
- Issue
- 1
- Pages
- 73-88
- ISSN
- 1040-4651 (ISSN-L)
- 1532-298X
- OpenAlex
- W2117880514
- Cited by
- 512
- References
- 44
- FWCI
- 5.97
- Type
- journal article
- Language
- en
- Open access
- green
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- Guo, D., Chen, F., Inoue, K., Blount, J. W., & Dixon, R. A. (2001). Downregulation of Caffeic Acid 3-O-Methyltransferase and Caffeoyl CoA 3-O-Methyltransferase in Transgenic Alfalfa: Impacts on Lignin Structure and Implications for the Biosynthesis of G and S Lignin. The Plant Cell. https://doi.org/10.1105/tpc.13.1.73
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