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Dynamic Analyses of the Expression of the HISTONE::YFP Fusion Protein in Arabidopsis Show That Syncytial Endosperm Is Divided in Mitotic Domains
Open accessAbstract
During early seed development, nuclear divisions in the endosperm are not followed by cell division, leading to the development of a syncytium. The simple organization of the Arabidopsis endosperm provides a model in which to study the regulation of the cell cycle in relation to development. To monitor nuclear divisions, we constructed a HISTONE 2B::YELLOW FLUORESCENT PROTEIN gene fusion (H2B::YFP). To validate its use as a vital marker for chromatin in plants, H2B::YFP was expressed constitutively in Arabidopsis. This enabled the observation of mitoses in living root meristems. H2B::YFP was expressed specifically in Arabidopsis syncytial endosperm by using GAL4 transactivation. Monitoring mitotic activity in living syncytial endosperm showed that the syncytium was organized into three domains in which nuclei divide simultaneously with a specific time course. Each mitotic domain has a distinct spatiotemporal pattern of mitotic CYCLIN B1;1 accumulation. The polar spatial organization of the three mitotic domains suggests interactions between developmental mechanisms and the regulation of the cell cycle.
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Cited by
The RETINOBLASTOMA-RELATED Gene Regulates Stem Cell Maintenance in Arabidopsis Roots
Wildwater, M., Campilho, A., Perez-Perez, J. M., Heidstra, R., Blilou, I., Korthout, H., Chatterjee, J., Mariconti, L., Gruissem, W., & Scheres, B. (2005) · Cell · doi:10.1016/j.cell.2005.09.042 · Cited by 395
Reddy, G. V., Heisler, M. G., Ehrhardt, D. W., & Meyerowitz, E. M. (2004) · Development · doi:10.1242/dev.01261 · Cited by 339
Zhang, X., De Marcos Lousa, C., Schutte-Lensink, N., Ofman, R., Wanders, R. J., Baldwin, S. A., Baker, A., Kemp, S., & Theodoulou, F. L. (2011) · Biochemical Journal · doi:10.1042/bj20110249 · Cited by 43
Metadata
- Authors
- Boisnard-Lorig, C., Colon-Carmona, A., Bauch, M., Hodge, S., Doerner, P., Bancharel, E., Dumas, C., Haseloff, J., & Berger, F.
- Venue
- The Plant Cell
- Publisher
- Oxford University Press (OUP)
- Published
- 2001-03
- Volume
- 13
- Issue
- 3
- Pages
- 495-509
- ISSN
- 1040-4651 (ISSN-L)
- 1532-298X
- OpenAlex
- W2127481448
- Cited by
- 369
- References
- 0
- FWCI
- 26.49
- Type
- journal article
- Language
- en
- Open access
- bronze
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- CoChat. (2026). Citation check: Dynamic Analyses of the Expression of the HISTONE::YFP Fusion Protein in Arabidopsis Show That Syncytial Endosperm Is Divided in Mitotic Domains. https://app.cochat.ai/papers/10.1105/tpc.13.3.495
- The paper
- Boisnard-Lorig, C., Colon-Carmona, A., Bauch, M., Hodge, S., Doerner, P., Bancharel, E., Dumas, C., Haseloff, J., & Berger, F. (2001). Dynamic Analyses of the Expression of the HISTONE::YFP Fusion Protein in Arabidopsis Show That Syncytial Endosperm Is Divided in Mitotic Domains. The Plant Cell. https://doi.org/10.1105/tpc.13.3.495
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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