The DOI matches its Crossref record; 81 of the 81 references it cites were found in a registry or an index.
Protein Degradation Rate in Arabidopsis thaliana Leaf Growth and Development
Open accessAbstract
We applied 15N labeling approaches to leaves of the Arabidopsis thaliana rosette to characterize their protein degradation rate and understand its determinants. The progressive labeling of new peptides with 15N and measuring the decrease in the abundance of >60,000 existing peptides over time allowed us to define the degradation rate of 1228 proteins in vivo. We show that Arabidopsis protein half-lives vary from several hours to several months based on the exponential constant of the decay rate for each protein. This rate was calculated from the relative isotope abundance of each peptide and the fold change in protein abundance during growth. Protein complex membership and specific protein domains were found to be strong predictors of degradation rate, while N-end amino acid, hydrophobicity, or aggregation propensity of proteins were not. We discovered rapidly degrading subunits in a variety of protein complexes in plastids and identified the set of plant proteins whose degradation rate changed in different leaves of the rosette and correlated with leaf growth rate. From this information, we have calculated the protein turnover energy costs in different leaves and their key determinants within the proteome.
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Cites
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Cited by
The nitrogen cost of photosynthesis
Evans, J. R., & Clarke, V. C. (2019) · Journal of Experimental Botany · doi:10.1093/jxb/ery366 · Cited by 438
Chloroplast Translation: Structural and Functional Organization, Operational Control, and Regulation
Zoschke, R., & Bock, R. (2018) · The Plant Cell · doi:10.1105/tpc.18.00016 · Cited by 285
Heinemann, B., Künzler, P., Eubel, H., Braun, H.-P., & Hildebrandt, T. M. (2020) · Plant Physiology · doi:10.1093/plphys/kiaa050 · Cited by 87
Metadata
- Authors
- Li, L., Nelson, C. J., Trösch, J., Castleden, I., Huang, S., & Millar, A. H.
- Venue
- The Plant Cell
- Publisher
- Oxford University Press (OUP)
- Published
- 2017-01-30
- Volume
- 29
- Issue
- 2
- Pages
- 207-228
- ISSN
- 1040-4651 (ISSN-L)
- 1532-298X
- OpenAlex
- W2582331638
- Cited by
- 258
- References
- 81
- FWCI
- 10.39
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Li, L., Nelson, C. J., Trösch, J., Castleden, I., Huang, S., & Millar, A. H. (2017). Protein Degradation Rate in Arabidopsis thaliana Leaf Growth and Development. The Plant Cell. https://doi.org/10.1105/tpc.16.00768
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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