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Antisense suppression of 4-coumarate:coenzyme A ligase activity in Arabidopsis leads to altered lignin subunit composition.
Open accessAbstract
The phenylpropanoid enzyme 4-coumarate:coenzyme A ligase (4CL) is considered necessary to activate the hydroxycinnamic acids for the biosynthesis of the coniferyl and sinapyl alcohols subsequently polymerized into lignin. To clarify the role played by 4CL in the biosynthesis of the guaiacyl (G) and syringyl (S) units characteristic of angiosperm lignin, we generated 4CL antisense Arabidopsis lines having as low as 8% residual 4CL activity. The plants had decreases in thioglycolic acid-extractable lignin correlating with decreases in 4CL activity. Nitrobenzene oxidation of cell walls from bolting stems revealed a significant decrease in G units in 4CL-suppressed plants; however, levels of S lignin units were unchanged in even the most severely 4CL-suppressed plants. These effects led to a large decrease in the G/S ratio in these plants. Our results suggest that an uncharacterized metabolic route to sinapyl alcohol, which is independent of 4CL, may exist in Arabidopsis. They also demonstrate that repression of 4CL activity may provide an avenue to manipulate angiosperm lignin subunit composition in a predictable manner.
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Cited by
Boerjan, W., Ralph, J., & Baucher, M. (2003) · Annual Review of Plant Biology · doi:10.1146/annurev.arplant.54.031902.134938 · Cited by 4,504
The Phenylpropanoid Pathway in Arabidopsis
Fraser, C. M., & Chapple, C. (2011) · The Arabidopsis Book · doi:10.1199/tab.0152 · Cited by 860
Self-citationLignins and lignification: Selected issues
Boudet, A.-M. (2000) · Plant Physiology and Biochemistry · doi:10.1016/s0981-9428(00)00166-2 · Cited by 377
First report of CRISPR/cas9 mediated dna-free editing of 4cl and rve7 genes in chickpea protoplasts
Badhan, S., Ball, A. S., & Mantri, N. (2021) · International Journal of Molecular Sciences · doi:10.3390/ijms22010396 · Cited by 193
Firon, N., LaBonte, D., Villordon, A., Kfir, Y., Solis, J., Lapis, E., Perlman, T., Doron-Faigenboim, A., Hetzroni, A., Althan, L., et al. (2013) · BMC Genomics · doi:10.1186/1471-2164-14-460 · Cited by 182
Metadata
- Authors
- Lee, D., Meyer, K., Chapple, C., & Douglas, C. J.
- Venue
- The Plant Cell
- Publisher
- Oxford University Press (OUP)
- Published
- 1997-11
- Volume
- 9
- Issue
- 11
- Pages
- 1985-1998
- ISSN
- 1040-4651 (ISSN-L)
- 1532-298X
- OpenAlex
- W2118316134
- Cited by
- 257
- References
- 0
- FWCI
- 11.96
- Type
- journal article
- Language
- en
- Open access
- green
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- CoChat. (2026). Citation check: Antisense suppression of 4-coumarate:coenzyme A ligase activity in Arabidopsis leads to altered lignin subunit composition. https://app.cochat.ai/papers/10.1105/tpc.9.11.1985
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- Lee, D., Meyer, K., Chapple, C., & Douglas, C. J. (1997). Antisense suppression of 4-coumarate:coenzyme A ligase activity in Arabidopsis leads to altered lignin subunit composition. The Plant Cell. https://doi.org/10.1105/tpc.9.11.1985
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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