A wide-angle X-ray fibre diffraction method for quantifying collagen orientation across large tissue areas: application to the human eyeball coat
Open accessAbstract
A quantitative map of collagen fibril orientation across the human eyeball coat, including both the cornea and the sclera, has been obtained using a combination of synchrotron wide-angle X-ray scattering (WAXS) and three-dimensional point mapping. A macromolecular crystallography beamline, in a custom-modified fibre diffraction setup, was used to record the 1.6 nm intermolecular equatorial reflection from fibrillar collagen at 0.5 mm spatial resolution across a flat-mounted human eyeball coat. Fibril orientation, derived as an average measure of the tissue thickness, was quantified by extraction of the azimuthal distribution of WAXS scatter intensity. Vector plots of preferential fibre orientation were remapped onto an idealized eyeball surface using a custom-built numerical algorithm, to obtain a three-dimensional representation of the collagen fibril architecture.
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X-ray scattering has shown that collagen lamellae in the human cornea have preferred orientations: inferior-superior and nasal-temporal in the central cornea and circumferential at the limbus.
“X-ray scattering has shown that the lamellae have preferred orientations in the human cornea: inferior-superior and nasal-temporal in the central cornea and circumferential at the limbus.”
From Structural control of corneal transparency, refractive power and dynamics (2025) · cites this paper as [12]
Throughout the optical zone, there is a preference for lamellae to run in the inferior-superior and temporal-nasal directions.
“Throughout the optical zone, there is a preference for lamellae to run in the inferior-superior and temporal-nasal directions.”
From Structural control of corneal transparency, refractive power and dynamics (2025) · cites this paper as [12]
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Cites
X-Ray Scattering Used to Map the Preferred Collagen Orientation in the Human Cornea and Limbus
Aghamohammadzadeh, H., Newton, R. H., & Meek, K. M. (2004) · Structure · doi:10.1016/j.str.2004.01.002 · Cited by 233 · see citing works
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Research works printed but not linked: 3
- Coudrillier, B., Boote, C., Quigley, H. A. & Nguyen, T. D. (2012). Biomech. Model Mechanobiol. doi:10.1007/s10237-012-0455-y. · DOI
- Maurice, D. M. (1988). The Cornea: Transactions of the World Congress on the Cornea III, edited by H. D. Cavanagh, pp. 187-192. New York: Raven Press.
- Meek, K. M. (2008). Collagen: Structure and Mechanics, edited by P. Fratzl, pp. 359-396. New York: Springer.
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Structural control of corneal transparency, refractive power and dynamics
Meek, K. M., Knupp, C., Lewis, P. N., Morgan, S. R., & Hayes, S. (2025) · Eye · doi:10.1038/s41433-024-02969-7 · Cited by 45 · see citing works
- Authors
- Pijanka, J. K., Abass, A., Sorensen, T., Elsheikh, A., & Boote, C.
- Affiliations
- Cardiff University (GB)
- University of Liverpool (GB)
- Moorfields Eye Hospital NHS Foundation Trust (GB)
- Venue
- Journal of Applied Crystallography
- Published
- 2013-09-18
- Volume
- 46
- Issue
- 5
- Pages
- 1481-1489
- ISSN
- 0021-8898 (ISSN-L)
- OpenAlex
- W2144605396
- Cited by
- 38
- References
- 1
- Citations by year
- 2026: 2 · 2025: 2 · 2024: 1 · 2023: 2 · 2022: 3 · 2021: 3 · 2020: 2 · 2019: 5 · 2018: 9 · 2017: 4 · 2015: 3 · 2014: 2
- FWCI
- 0.64
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Journal of Applied Crystallography (publishedVersion, cc-by)
- ORCA Online Research @Cardiff (Cardiff University) (submittedVersion)
- Funders
- National Institute for Health and Care Research
- Moorfields Eye Hospital NHS Foundation Trust
- Topics
- Physical Sciences › Materials Science › Biomaterials › Collagen: Extraction and Characterization
- Physical Sciences › Physics and Astronomy › Radiation › Advanced X-ray Imaging Techniques
- Physical Sciences › Materials Science › Materials Chemistry › X-ray Diffraction in Crystallography
- Keywords
- collagen fibril orientation, wide-angle X-ray scattering, fiber diffraction, cornea, sclera, fibrillar collagen, azimuthal distribution, human eye
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