Measurement of Dielectric Properties of Microalgae with Different Lipid Content Using Electrorotation and Negative Dielectrophoresis Cell Trap
Abstract
This paper presents a precise measurement of dielectric properties of cells, especially microalgae having different intracellular lipid content by using a combination of electrorotation (ROT) and negative dielectrophoresis (nDEP) force-based cell trapping method. To achieve this, eight electrode pairs were built within a microchannel, sharing the same geometrical center. A single microalgal cell was trapped and levitated in the center by nDEP force generated from four electrode pairs, while simultaneously ROT torque was generated from the other four pairs to rotate the trapped cell. This method allows conducting ROT measurement at up to ~100 MHz on a single trapped cell. As a result, both the cytoplasm conductivity and permittivity that depends on intracellular lipid content differences could be accurately analyzed based on the rotation speed of the cell.
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Investigation of the dielectric properties of micro-algae using microsystem technology
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- Authors
- Han, S.-I., Huang, C., & Han, A.
- Affiliations
- Texas A&M University (US)
- Venue
- 2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems & Eurosensors XXXIII (TRANSDUCERS & EUROSENSORS XXXIII)
- Publisher
- IEEE
- Published
- 2019-06
- Pages
- 960-963
- ISSN
- 2167-0021 (ISSN-L)
- OpenAlex
- W2969358145
- Cited by
- 9
- References
- 10
- Citations by year
- 2023: 2 · 2022: 3 · 2021: 2 · 2020: 1
- FWCI
- 3.11
- Type
- conference paper
- Language
- en
- Open access
- closed
- Licence
- Locations
- International Conference on Solid-State Sensors, Actuators and Microsystems (publishedVersion)
- Funders
- National Science Foundation
- Army Research Laboratory
- Topics
- Physical Sciences › Engineering › Biomedical Engineering › Microfluidic and Bio-sensing Technologies
- Physical Sciences › Engineering › Electrical and Electronic Engineering › Microwave and Dielectric Measurement Techniques
- Physical Sciences › Physics and Astronomy › Atomic and Molecular Physics, and Optics › Digital Holography and Microscopy
- Keywords
- microalgae, dielectric properties, electrorotation, negative dielectrophoresis, cell trapping, intracellular lipid content, cytoplasm conductivity, dielectric constant, single-cell measurement, cell rotation
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