Structural basis of pheromone binding to mouse major urinary protein (MUP‐I)
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The mouse major urinary proteins are pheromone‐binding proteins that function as carriers of volatile effectors of mouse physiology and behavior. Crystal structures of recombinant mouse major urinary protein‐I (MUP‐I) complexed with the synthetic pheromones, 2‐sec‐butyl‐4,5‐dihydrothiazole and 6‐hydroxy‐6‐methyl‐3‐heptanone, have been determined at high resolution. The purification of MUP‐I from mouse liver and a high‐resolution structure of the natural isolate are also reported. These results show the binding of 6‐hydroxy‐6‐methyl‐3‐heptanone to MUP‐I, unambiguously define ligand orientations for two pheromones within the MUP‐I binding site, and suggest how different chemical classes of pheromones can be accommodated within the MUP‐I β‐barrel.
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MUP-I's ability to bind different lipophilic ligands derives from limited conformational flexibility and unoccupied space in the hydrophobic β-barrel interior.
“In this case, the ability of MUP-I to bind different lipophilic ligands derives from a limited extent of conformational flexibility and unoccupied space within the hydrophobic interior of the β-barrel.413”
From Protein Ensembles: How Does Nature Harness Thermodynamic Fluctuations for Life? The Diverse Functional Roles of Conformational Ensembles in the Cell (2016) · cites this paper as [413]
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- Authors
- Timm, D. E., Baker, L. J., Mueller, H., Zidek, L., & Novotny, M. V.
- Affiliations
- Indiana University Indianapolis (US)
- Corresponding author
- Timm, David E.
- Venue
- Protein Science
- Publisher
- Wiley
- Published
- 2001-05
- Volume
- 10
- Issue
- 5
- Pages
- 997-1004
- ISSN
- 0961-8368 (ISSN-L)
- 1469-896X
- PMC
- PMC2374202
- OpenAlex
- W2016271327
- Cited by
- 109
- References
- 32
- Citations by year
- 2026: 2 · 2025: 1 · 2024: 4 · 2023: 5 · 2022: 3 · 2021: 2 · 2020: 4 · 2019: 5 · 2018: 2 · 2017: 8 · 2016: 8 · 2015: 5 · 2014: 4 · 2013: 6 · 2012: 1
- FWCI
- 2.83
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Protein Science (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health (5r01dc002418-07, 1r01dk054738-01a1)
- National Institute on Deafness and Other Communication Disorders (DC 02418)
- National Institute of General Medical Sciences (GM 55055, R01GM055055, R29GM055055)
- National Institute of Diabetes and Digestive and Kidney Diseases (DK-54738)
- Topics
- Life Sciences › Neuroscience › Sensory Systems › Olfactory and Sensory Function Studies
- Physical Sciences › Materials Science › Materials Chemistry › Enzyme Structure and Function
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Interaction Studies and Fluorescence Analysis
- Keywords
- pheromone binding, pheromone-binding proteins, ligand binding sites, beta-barrel proteins, protein-ligand complex structure, mouse liver, protein purification
- MeSH terms
- Animals, Binding Sites, Hydrogen Bonding, Liver, Models, Molecular, Pheromones, Protein Binding, Proteins, Structure-Activity Relationship, Protein Structure, Secondary, Crystallography, X-Ray, Mice
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