Cited by 109Olfactory and Sensory Function StudiesEnzyme Structure and FunctionProtein Interaction Studies and Fluorescence Analysis

Structural basis of pheromone binding to mouse major urinary protein (MUP‐I)

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Timm, David E., Baker, L.J., Mueller, Heather, Zidek, Lukas, Novotny, Milos V.·2001·Protein Science

Abstract

The mouse major urinary proteins are pheromone‐binding proteins that function as carriers of volatile effectors of mouse physiology and behavior. Crystal structures of recombinant mouse major urinary protein‐I (MUP‐I) complexed with the synthetic pheromones, 2‐sec‐butyl‐4,5‐dihydrothiazole and 6‐hydroxy‐6‐methyl‐3‐heptanone, have been determined at high resolution. The purification of MUP‐I from mouse liver and a high‐resolution structure of the natural isolate are also reported. These results show the binding of 6‐hydroxy‐6‐methyl‐3‐heptanone to MUP‐I, unambiguously define ligand orientations for two pheromones within the MUP‐I binding site, and suggest how different chemical classes of pheromones can be accommodated within the MUP‐I β‐barrel.

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Authors
Timm, D. E., Baker, L. J., Mueller, H., Zidek, L., & Novotny, M. V.
Affiliations
Indiana University Indianapolis (US)
Corresponding author
Timm, David E.
Venue
Protein Science
Publisher
Wiley
Published
2001-05
Volume
10
Issue
5
Pages
997-1004
ISSN
  • 0961-8368 (ISSN-L)
  • 1469-896X
OpenAlex
W2016271327
Cited by
109
References
32
Citations by year
2026: 2 · 2025: 1 · 2024: 4 · 2023: 5 · 2022: 3 · 2021: 2 · 2020: 4 · 2019: 5 · 2018: 2 · 2017: 8 · 2016: 8 · 2015: 5 · 2014: 4 · 2013: 6 · 2012: 1
FWCI
2.83
Type
journal article
Language
en
Open access
Locations
  • Protein Science (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • National Institutes of Health (5r01dc002418-07, 1r01dk054738-01a1)
  • National Institute on Deafness and Other Communication Disorders (DC 02418)
  • National Institute of General Medical Sciences (GM 55055, R01GM055055, R29GM055055)
  • National Institute of Diabetes and Digestive and Kidney Diseases (DK-54738)
Topics
  • Life Sciences › Neuroscience › Sensory Systems › Olfactory and Sensory Function Studies
  • Physical Sciences › Materials Science › Materials Chemistry › Enzyme Structure and Function
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Interaction Studies and Fluorescence Analysis
Keywords
pheromone binding, pheromone-binding proteins, ligand binding sites, beta-barrel proteins, protein-ligand complex structure, mouse liver, protein purification
MeSH terms
Animals, Binding Sites, Hydrogen Bonding, Liver, Models, Molecular, Pheromones, Protein Binding, Proteins, Structure-Activity Relationship, Protein Structure, Secondary, Crystallography, X-Ray, Mice

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